Activity of invertase in immobilized cells of carrot

Micieta, K. . Prirodovedecka Fakulta; Stano, J. . Farmaceuticka Fakulta; Blanarikova, V.; Havranek, E.; Andriamainty, F.; Birjukova, N.; Ignatova, S.; Safarik, I.

Activity of invertase in immobilized cells of carrot

1999

Cell suspension cultures of carrot (Daucus carota L. cv. Kalisto) were permeabilized with Tween 20, Tween 80, 30% ethanol, 50% ethanol, and hexadecyltrimethylammonium bromide and immobilized with glutaraldehyde. Invertase of cell suspension culture and of immobilized cells showed its activity optimum at pH 4.5 and temperature 53 deg C. Enzyme hydrolysis was linear within 4 h, reaching 66% conversion. The immobilized cells are characterised by a high invertase activity and showed convenient mechanical properties

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AGROVOC Keywords

culture techniques daucus carota enzimas inmovilizadas fructofuranosidasa fructofuranosidase immobilisation immobilization immobilized enzymes technique de culture

Bibliographic information

Published in

Bulletin Potravinarskeho Vyskumu (Slovak Republic) | Bulletin of Food Research

Volume 38 Issue 3 ISSN 0231-9950

Pagination

pp. 153-161

Other Subjects

Inmovilizacion; Enzyme immobilisee; Tecnicas de cultivo

Language

Slovak

Note

Summaries (En, Sk)

3 tables, 1 ill.; 30 ref.

Translated Title

Aktivita invertazy v imobilizovanych bunkach mrkvy

Type

Summary

In AGRIS since: 1999-08-15

Format: AGRIS AP

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Activity of invertase in immobilized cells of carrot

1999

AGROVOC Keywords

Bibliographic information