The identification of breed-specific and individual-specific RAPD markers in sheep
1999
Erceg, S. ("Vinca" Institute of Nuclear Sciences, Belgrade (Yugoslavia)) | Petrovic, M.P. | Trenkovska, S. | Alavantic, D.
The Random Amplified Polymorphic DNA (RAPD) method utilizes low stringency PCR amplification with single primer of arbitrary sequence to amplify strain-specific arrays of anonymous DNA fragments. In this study, the RAPD assay was applied to identify polymorphic markers in three sheep breeds (Pirotska Pramenka -P, Il de France - F, and Wuertenberg - W) and their crosses. Using 12 different primers we obtained 70 RAPD markers. Thirty-eight of them are present in all breeds, thirteen are individual-specific, and nineteen are polymorphic for different sheep breeds. These markers could be useful for further research according molecular parameters in sheep selection area.
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