Metabolite inhibitory to aflatoxigenic fungi produced by Cladosporium fulvum Cooke
1994
Divina, R.D. | Mabesa, R.C. | Garcia, V.V. | Nakajima, H. | Hamasaki, T. (Central Luzon State Univ., Munoz, Nueva Ecija (Philippines))
An inhibitory metabolite against aflatoxigenic fungi was isolated and purified from the culture broth of Cladosporium fulvum. The culture filtrate was acidified to pH 3.5-4.5, added with activated charcoal (2% w/v) for 24 hours and then filtered. Charcoal was extracted with acetone or methanol and the extract was washed with n-hexane and passed through a column of Sephadex LH-20. Final purification was achieved by high performance liquid chromatography (HPLC) which produced a residue of 6.2 mg/l culture broth. The active compound was partially characterized based on its ultraviolet (UV), infrared (IR), proton NMR (H-NMR) and M5 spectra. The results showed that the metabolite inhibited the growth of Aspergillus parasiticus, A. flavus and Alternaria kikuchiana
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