Reactivity of adenosine triphosphatase adsorbed on nitrocellulose

Momic, T.; Banjac, A.; Horvat, A. . Laboratorija za molekularnu biologiju

Reactivity of adenosine triphosphatase adsorbed on nitrocellulose

2001

Momic, T. | Banjac, A. | Horvat, A. (Institut za nuklearna istrazivanja Vinca, Beograd (Yugoslavia). Laboratorija za molekularnu biologiju)

Possibilities of application of transmembrane enzymes as a biological component of a biosensor for water quality control and detection of toxical substances were performed. Synaptic plasma membranes (SPMs) were adsorbed on nitrocellulose filters. The adsorption of SPMs was followed by determination of the transmembrane enzyme Na,K-ATPase. The optimal conditions for SPM adsorption on nitrocellulose filters were determined: 25 microgram per nitrocellulose filter disc during 1 hour of incubation, on - 20 deg C. The ATPase activity of adsorbed SPM showed, that almost 30% of enzymic activity was detected on nitrocellulose filters in mentioned conditions. This results showed that adsorption of SPM on solid support enhancing enzymatic stability and enable its industrial and analytical application.

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AGROVOC Keywords

adsorption atpasa atpase biosensores biosensors calidad del agua enzimas inmovilizadas immobilized enzymes membrana membrane membranes water quality

Bibliographic information

Publisher

Jugoslovensko drustvo za zastitu voda

Pagination

p. 351-354

Other Subjects

Biodetecteur; Adsorcion; Qualite de l'eau; Enzyme immobilisee

Language

Serbian

Note

Summaries (En, Sr)

1 graph; 13 ref.

Translated Title

Aktivnost adenozin trifosfataza adsorbovanih na nitrocelulozi

Type

Summary; Conference

Source

"Water pollution control 2001": the 30th annual conference of Yugoslav Water Pollution Control Society. Conference proceedings, Djukic, A. (Ed.).- Beograd (Yugoslavia): Jugoslovensko drustvo za zastitu voda, 2001. 417 p.

Conference

30. konferencija o aktuelnim problemima zastite voda Zastita voda 2001, Arandjelovac (Yugoslavia), 12-15 Jun 2001

In AGRIS since: 2002-08-15

Format: AGRIS AP

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