Reactivity of adenosine triphosphatase adsorbed on nitrocellulose

Momic, T.; Banjac, A.; Horvat, A. . Laboratorija za molekularnu biologiju

Reactivity of adenosine triphosphatase adsorbed on nitrocellulose

2001

Momic, T. | Banjac, A. | Horvat, A. (Institut za nuklearna istrazivanja Vinca, Beograd (Yugoslavia). Laboratorija za molekularnu biologiju)

Possibilities of application of transmembrane enzymes as a biological component of a biosensor for water quality control and detection of toxical substances were performed. Synaptic plasma membranes (SPMs) were adsorbed on nitrocellulose filters. The adsorption of SPMs was followed by determination of the transmembrane enzyme Na,K-ATPase. The optimal conditions for SPM adsorption on nitrocellulose filters were determined: 25 microgram per nitrocellulose filter disc during 1 hour of incubation, on - 20 deg C. The ATPase activity of adsorbed SPM showed, that almost 30% of enzymic activity was detected on nitrocellulose filters in mentioned conditions. This results showed that adsorption of SPM on solid support enhancing enzymatic stability and enable its industrial and analytical application.

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Ключевые слова АГРОВОК

adsorption atpasa atpase biosensores biosensors calidad del agua enzimas inmovilizadas immobilized enzymes membrana membrane membranes water quality

Библиографическая информация

Издатель

Jugoslovensko drustvo za zastitu voda

Нумерация страниц

p. 351-354

Другие темы

Biodetecteur; Adsorcion; Qualite de l'eau; Enzyme immobilisee

Язык

сербский

Примечание

Summaries (En, Sr)

1 graph; 13 ref.

Переведенный заголовок

Aktivnost adenozin trifosfataza adsorbovanih na nitrocelulozi

Тип

Summary; Conference

Источник

"Water pollution control 2001": the 30th annual conference of Yugoslav Water Pollution Control Society. Conference proceedings, Djukic, A. (Ed.).- Beograd (Yugoslavia): Jugoslovensko drustvo za zastitu voda, 2001. 417 p.

Конференция

30. konferencija o aktuelnim problemima zastite voda Zastita voda 2001, Arandjelovac (Yugoslavia), 12-15 Jun 2001

В АГРИСе с: 2002-08-15

Формат: AGRIS AP

Поставщик данных

Эту запись предоставил Unassigned data from Yugoslavia

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