Genetic Diversity among Waxy Corn Inbred Lines Revealed by CACTA-TD Markers
2004
Keeping, Andrew (University of Toronto, Toronto, Ontario, Canada) | Lee, J.K. (Kangwon National University, Chunchon, Republic of Korea) | Park, J.Y. (Kangwon Agricultural Research and Extension Services, Hongcheon, Republic of Korea) | Kwon, S.J. (Kangwon National University, Chunchon, Republic of Korea) | Kim, N.S. (Kangwon National University, Chunchon, Republic of Korea), E-mail: [email protected] | Park, K.J. (Kangwon Agricultural Research and Extension Services, Hongcheon, Republic of Korea) | Min, H.K. (Kangwon Agricultural Research and Extension Services, Hongcheon, Republic of Korea)
CACTA is a class 2 transposon superfamily found exclusively in plants. In this study, we have applied CACTA-TD technique to study the genetic diversity among 90 waxy corn inbred lines. While CACTA-TD fragments ranged approximately from 100 to 650 bp in size, the fragments in the range of 100-500 bp showed high profiles of polymorphisms. Analysis of the 90 inbred lines with 5 CACTA-TD primer combinations detected a total of 204 fragments, of which 165 (80.9%) were polymorphic among the inbreds.
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