Somatic embryogenesis and organogenesis in Philippine improved sweet potato (Ipomoea batatas L.( Lam) varieties
2005
Carpena, C.L.(Philippines Univ. Los Baños, College, Laguna (Philippines). Dept. of Agronomy. Dacumos, C.N.)
English. ten NSIC recommended varieties of sweet potato (UPL Sp-1, UPL Sp-2, UPL Sp-3, UPL Sp-5, PSB Sp-14, PSB Sp-17, PSB Sp-18, PSB Sp-21, NSIC Sp-23 and NSIC Sp-24) were evaluated for somatic embryogenesis and/or oragnogenesis using meristem, leaf, petiole and nodal cuttings as explants. Culture media used were MS and LS with varying concentrations of 2,4-D or 4-FA for callus induction and with different amounts of BAP, GA3 or ABA for plant regeneration. Meristem cultures of UPL Sp-5 in MS medium produced a 1:3 ratio of embryogenic to organogenic calli while meristem cultures of PSB Sp-17, NSIC Sp-23 and NSIC Sp-24 produced only organic calli. Regeneration frequencies from these varieties ranged from 0.35% (PSB Sp-17) to 25% (UPL Sp-5) of the calli produced. leaf cultures of five varieties (UPL Sp-2, UPL Sp-3, UPL Sp-5, PSB Sp-17 and NSIC Sp-23) in LS medium produced only organogenic calli ranging from 16% (UPL Sp-3) to 90% (UPL Sp-5) while leaf cult ures of NSIC Sp-23 in the same medium produced up to 30% embryogenic calli. No regenerants were, however, obtained from any of the calli from these leaf cultures.
Show more [+] Less [-]English. Scientific Conference of the federation of Crop Science Societies of the Philippines Lapasan, Cagayan de Oro City (Philippines) 2-6 May 2005
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