[Production of a polyfunctional diagnosticum to detect infectious agents]
2008
Zharnikova, I.V. | Zharnikova, T.V. | Belik, E.V. | Gryadskikh, T.A. | Samarina, I.V. | Kochiaryan, A.S., Stavropol State Agrarian Univ. (Russian Federation) | Brykalov, A.V. | Golovkina, E.M.
A technology of producing a suspension fluorescent enzymatic diagnosticum was developed to detect infectious agents antigens in a suspension agglutination test (SAT), immunofluorescence test (IFT) and EIA. A characteristic of this polyfunctional diagnosticum is given and its efficacy is shown compared to the conventional diagnosticums: the test system for EIA, fluorescing immunoglobulins (IG) for IFT and suspension diagnosticum for HNT. The diagnosticum production technology is based on binding immunoglobulin ligand (class G IG) with aluminium silicate (a medium) characterized by high reactivity of surface centers. As a dye fluorescein isothiocyanate was used. Testing tularemia diagnosticum (TD) and leptospirosis diagnosticum (LD) compared to erythrocytic TD in HNT, suspension (dye – auramin) in CFT, tularemia diagnostic fluorescent IG in IFT and immunoperoxidase conjugate in EIA showed high activity and specificity of the polyfunctional diagnosticum allowing to make an express diagnosis in CFT in 1-5 min (in IFT – 40 min, in EIA – 3 h). In EIA the results similar to the conventional diagnosticum were obtained, but the shelf life of the diagnosticum increased twice (2 years, not 1 year). An indefeasible advantage of the polyfuctional diagnosticum is a possibility to run simultaneously 3 kinds of express analyses (CFT, IFT and EIA) having the comparative sensibility when using only one ampoule of the preparation in field conditions and extreme epizootic and epidemic situations.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Central Scientific Agricultural Library