Detection of ceratocystis fimbriata from fig tree and soil by the loop-mediated isothermal amplification (LAMP) method
2009
Fukuta, S., Aichi-ken. Agricultural Research Center, Nagakute (Japan) | Miyake, N. | Mashita, N. | Yamada, M.
Loop-mediated isothermal amplification (LAMP) to detect the causal agent of Ceratocystis canker, Ceratocystis fimbriata was developed. Specific primers were designed by targeting the ITS region. The detection sensitivity of LAMP was 0.1 pg of C. fimbriata DNA. With LAMP methods, C. fimbriata could be detected from fig tree and soil samples that were prepared from fig fields in Aichi prefecture.
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