Establishment of somatic cell clones in Thesium chinense Turcz and its in vitro rooting technique
2008
Jiang Qin | Dong Ling | Ning Zhiyuan | Chen Jingxian | Liao Huajun
[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz. [Method] With stem fragments of wild Thesium chinense Turcz as explants, different culture media were designed to conduct induction culture, strengthening plantlet culture and in vitro rooting. [Result] The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA, 0.01 mg/L NAA and 0.3 mg/L 2,4-D; in addition, 60 mg/kg ABT was suitable for rooting, by which the percentage of rooted plantlets reached 76.6%. [Conclusion] This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate, providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.
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