Establishment of somatic cell clones in Thesium chinense Turcz and its in vitro rooting technique
2008
Jiang Qin | Dong Ling | Ning Zhiyuan | Chen Jingxian | Liao Huajun
[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz. [Method] With stem fragments of wild Thesium chinense Turcz as explants, different culture media were designed to conduct induction culture, strengthening plantlet culture and in vitro rooting. [Result] The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA, 0.01 mg/L NAA and 0.3 mg/L 2,4-D; in addition, 60 mg/kg ABT was suitable for rooting, by which the percentage of rooted plantlets reached 76.6%. [Conclusion] This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate, providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Institute of Agricultural Information, Chinese Academy of Agricultural Sciences
Découvrez la collection de ce fournisseur de données dans AGRIS