Study on HPLC specific chromatogram of Capsicum chinense Jacq. in Hainan Province | 海南黄灯笼辣椒HPLC特征图谱研究_
2011
Li Guowei, Hainan Standard Biological Technology Co., Ltd., Haikou (China) | Ji Minghui, Hainan Normal University,Zhengzhou (China) | Guo Feiyan, Hainan Normal University,Zhengzhou (China)
Chinese. ［目的］建立黄灯笼辣椒的高效液相色谱特征图谱，为科学评价和有效控制海南黄灯笼辣椒提供新方法。［方法］收集6个产地2个季节的黄灯笼辣椒，采用高效液相色谱测定其特征图谱。色谱条件：十八烷基硅烷键合硅胶为填充剂，检测波长281 nm，以甲醇-水-磷酸（70.0∶30.0∶0.1）为流动相，流动速度1.0 ml/min。［结果］初步建立了黄灯笼辣椒的高效液相色谱特征图谱。［结论］高效液相色谱特征图谱重现性好，可作为黄灯笼辣椒的一项质控指标。_
Show more [+] Less [-]English. ［Objective］The aim was to establish HPLC specific chromatogram for the scientific evaluation and effective identification of Hainan Capsicum chinense Jacq.［Method］These Hainan Capsicum chinense Jacq were collected in two seasons in six regions respectively. And their fingerprints were examined by high-performance liquid chromatography. Chromatography conditions: HPLC was performed on C18 column with methanol-water-phosphoric acid (70∶30∶0.1) as mobile phase at flow rate of 1.0 ml/min. The detection wavelength was 281 nm.［Result］The HPLC specific chromatogram of Hainan _Capsicum chinense_ Jacq. was established preliminarily.［Conclusion］HPLC specific chromatogram method was repeatable and could be used in quality assessment of Hainan _Capsicum chinense_ Jacq._
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