Study of genetic diversity in maize (Zea may L.) inbred lines of Ningxia by SSR markers | 宁夏常用玉米自交系的SSR遗传分析_____
2011
Li Xin, Crop Institution of Ningxia Academy of Agricultural and Forestry Science, Yongning (China) | She Kuijun, Crop Institution of Ningxia Academy of Agricultural and Forestry Science, Yongning (China)
Chinese. ［目的］对宁夏常用的82份玉米（Zea may L.）自交系进行遗传分析，划分自交系类群，以提高玉米育种效率。［方法］利用SSR标记中可用于遗传多样性分析的20对SSR引物对材料进行分析。［结果］82份自交系中共检测出335个等位基因，其中具有多态性的为108个，每对引物检测出3～10个多态性位点，平均为5.4个。SSR引物的多态性信息量（PIC）介于0.523 3~0.858 9,平均为0.7123。［结论］ 82份自交系划分为5个类群，分类结果基本与系谱来源一致。_
Show more [+] Less [-]English. ［Objective］ In order to classify maize inbred genetic group and increase the breeding efficiency, the genetic variation of 82 maize inbred lines in Ningxia was analyzed. ［Method］ One types of molecular marker, simple sequence repeats (SSR) was assayed to determine the genetic diversity of these materials. ［Result］ 20 core-primers could test 335 allele sites in sample of 82 inbred lines, among them 108 were polymorphic. The average number of allele per SSR locus was 5.4 with arranging from 3 to 10. The average of polymorphic information content (PIC) was 0.712 3 with arranging from 0.523 3 to 0.858 9. ［Conclusion］ The inbred lines are classified into 5 groups and the results agree with the pedigree of the inbred line._
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