Zastosowanie metody HPLC do oznaczania zmian zawartosci glikopiranozydow podczas dojrzewania nasion bobiku.
1996
Nestorowicz J. | Pierzynowska Kornik G. | Zadernowski R.
Uridine was applied as an internal standard for the determination of glucopiranosides in faba bean seeds by the HPLC method. The relative UV response factors of vicine or convicine to uridine were determined. The changes in the content of vicine and convicine during ripening of bean seeds were followed. It was observed that with increase in the seed dry matter from 0.18 to 0.78 g/kg, to dry matter based content vicine decreased from 26.225 to 0.243 g/kg, and that of convicine from 9.01 to 0.094 g/kg.
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