Crystallization and preliminary X-ray diffraction studies of bacterial alginate lyase [from a soil bacterium Flavobacterium sp.].

Mikami B.; Hisano T.; Yamashita T.; Inose T.; Takagi M.; Sakaguchi K.; Kimura A.; Imanaka T.; Murata K.

Crystallization and preliminary X-ray diffraction studies of bacterial alginate lyase [from a soil bacterium Flavobacterium sp.].

1994

Alginate lyase (EC 4.2.2.3) from a soil bacterium (Flavobacterium sp.), which was overexpressed in Bacillus subtilis, has been crystallized by the vapor-diffusion method. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystal was resistant to X-ray radiation damage and diffracted to about 2.2A resolution. The diffraction pattern indicated that the crystal belongs to the orthorhombic system, space group P21-21-21 with unit-cell dimensions of a=82.7, b=142.1 and c=80.9 A. It is supposed that the asymmetric unit consists of two alginate lyase molecules.

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AGROVOC Keywords

alginate alginates alginatos bacillus subtilis cristallisation crystallization flavobacterium gene expression liasas lyase lyases optical properties rayon x rayos x x rays

Bibliographic information

Published in

Journal of Fermentation and Bioengineering

Volume 77 ISSN 0922-338X

Pagination

v.691-692(6)

Other Subjects

Expresion genica; Propriete optique; Propiedades opticas; Cristalizacion; Expression des genes

Language

English

Note

2 fig.; 19 ref. Summary (En).

Type

Journal Article; Journal Part; Summary

Source

Journal-of-Fermentation-and-Bioengineering (Japan). (Jun 1994). v. 77(6) p. 691-692.

Corporate Author

Kyoto Univ., Uji (Japan). Research Inst. for Food Science

Estonian Scientific Research Institute of Forestry and Nature Conservation, Tartu (Estonia).

In AGRIS since: 2012-11-15

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Crystallization and preliminary X-ray diffraction studies of bacterial alginate lyase [from a soil bacterium Flavobacterium sp.].

1994

AGROVOC Keywords

Bibliographic information