Crystallization and preliminary X-ray diffraction studies of bacterial alginate lyase [from a soil bacterium Flavobacterium sp.].
1994
Mikami B. | Hisano T. | Yamashita T. | Inose T. | Takagi M. | Sakaguchi K. | Kimura A. | Imanaka T. | Murata K.
Alginate lyase (EC 4.2.2.3) from a soil bacterium (Flavobacterium sp.), which was overexpressed in Bacillus subtilis, has been crystallized by the vapor-diffusion method. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystal was resistant to X-ray radiation damage and diffracted to about 2.2A resolution. The diffraction pattern indicated that the crystal belongs to the orthorhombic system, space group P21-21-21 with unit-cell dimensions of a=82.7, b=142.1 and c=80.9 A. It is supposed that the asymmetric unit consists of two alginate lyase molecules.
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