Chlamydospore Production Conditions of Gliocladium virens and It＇s Control Effect on Penicillium digitatum | 绿色粘帚霉厚垣孢子产生条件及其对柑橘绿霉病的防治效果研究
2013
Li Yong, Sichuan Agricultural University, Wenjiang(Sichuan),College of Agriculture | Wen Chengding, Sichuan Agricultural University, Wenjiang(Sichuan),College of Agriculture
Chinese. 【目的】以绿色粘帚霉菌株UV一44为对象，研究不同培养基、pH、培养时间和温度对厚垣孢子产量影响及其对柑橘绿霉病的室内防效。【方法】采用摇瓶震荡培养菌株，血球计数板计数厚垣孢子数；室内贮藏时浸果后单果独立包装，以清水和施宝克为对照，研究厚垣孢子悬液、分生孢子悬液和发酵原液对柑橘绿霉病的相对防效。【结果】Uv_44厚垣孢子产量最高的培养基、pH、培养时间和温度分别是燕麦片液体培养基、pH10、13d和30℃，该条件下产生厚垣孢子浓度为1．52×1011个／L；室内贮藏30d、60d和90d后，厚垣孢子相对防效随时间增加，分生孢子悬液和发酵原液低则逐渐降低。【结论】绿色粘帚霉厚垣孢子具有重要的生防价值。
Show more [+] Less [-]English. [Objective] The Gliocladium virens strain UV-44 was used as the object of study. Our aim was to investigate the effects of different culture mediums, pH, incubation time and tempera- ture on chlamydospore production and it＇s control effects on Penicilliurn digitatum. [Method] The strain was cultivated by shaking flask and the number of chlamydospore was calculated by he- mocytometer. The oranges were individually wrapped after dipping in solutions. The relative con- trol effects of chlamydospore suspension, conidia suspension and fermentation liquid on P. digita- turn were measured compared to controls （water and Prochloraz）. [Results] Under the conditions （oatmeal, pH 10, 13 d and 30 ~C）, the concentration of UV-44 chlamydospore was 1.52 X 10it A/ L, which value was the largest. The relative control effect increased with time after 30 d, 60 d and 90 d. However, the concentration of chlamydospore in conidial suspension and fermentation liquid were decreased gradually. [Conclusion] Chlamydospore of G. virens is valuable for biocontrol.
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