Effect of electromagnetic field on ultra high frequency of low power on the biosynthesis of biologically active substances in cell cultures of Silybum marianum L.
2015
Kopach,O.V. | Pushkina,N.V. | Kuzovkova,A.A. | Karpovich,V.A.
Russian. Исследовано воздействие электромагнитного излучения сверхвьгсокочастотного диапазона волн низкого уровня мощности (ЭМП СВЧ) на биосинтез биологически активных веществ в клеточных культурах расторопши пятнистой (Silybum marianum L.) белоцветковой расы. Установлен стимулирующий эффект вторичных метаболитов (флавоноидов и оксикоричных кислот) на биосинтез в каллусных клетках и определен возраст каллусов, при котором клетки более отзывчивы к воздействию ЭМП СВЧ низкой мощности.
Show more [+] Less [-]English. Medicinal plants appear a natural source of valuable biologically active substances, used in medicine, food and cosmetic industry. In vitro technology allow receiving plant biologically active substances out of cell and tissue culture irrespective from climatic and soil conditions all-year long saving natural resources undamaged. Electromagnetic field can be used for physical stimulation of biosynthesis in vitro culture increasing optimal cell growth and high content of secondary metabolites in biomass. The main goal of the research appears evaluation of the changes in metabolism of callus cultures of Silybum marianum upon the analysis of flavonoids and hydroxy-cinnamic acid content in cells after electromagnetic treatment. The stimulatory effect of ultra high frequency of low power electromagnetic field on the biosynthesis of secondary metabolites by the callus cultures of white flowers race Silybum marianum L. has been detected. The response level of the Silybum marianum L. callus cells has been noticed to be depended on the cell dedifferentiation degree.
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