Anti-Inflammatory Effect of Ethanolic Extract from Polyopes affinis through Suppression of NF-κB and MAPK Activation in LPS-Stimulated RAW 264.7 Cells
2017
Kim, M.J., Pukyong National University, Busan, Republic of Korea | Kim, K.B.W.R., Pukyong National University, Busan, Republic of Korea | Park, S.H., Pukyong National University, Busan, Republic of Korea | Park, S.Y., Pukyong National University, Busan, Republic of Korea | Choi, H.D., Pukyong National University, Busan, Republic of Korea | Choi, J.S., Kyungnam College of Information and Technology, Busan, Republic of Korea | Jang, M.R., Ministry of Food and Drug Safety, Cheongju, Republic of Korea | Im, M.H., Daegu University, Gyeongsan, Republic of Korea | Ahn, D.H., Pukyong National University, Busan, Republic of Korea
In this study, the anti-inflammatory effect of Polyopes affinis ethanol extract (PAEE) was investigated using LPS-stimulated RAW 264.7 cells and a croton oil-induced ICR mice model. Treatment with PAEE significantly reduced production of nitric oxide (NO) and pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor (TNF)-alpha, and IL-1beta] in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. PAEE treatment also reduced expression of inducible NO synthase, cyclooxygenase-2, nuclear factor-kB, and mitogen-activated protein kinases in LPS-stimulated RAW 264.7 cells. In the croton oil-induced ear edema test, application of PAEE (10~250 mg/kg body weight) reduced ear edema in a dose-dependent manner, and PAEE treatment at 50 mg/kg body weight showed similar inhibitory effects compared with prednisolone (10 mg/kg body weight). Histological analysis revealed reduced dermal thickness and lower number of infiltrated mast cells. These results suggest that PAEE might be used as a promising anti-inflammatory agent for inhibition of LPS-induced inflammation and ear edema formation.
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