Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber (Cucumis sativus L.)

Plader, W.; Hoshi, Y.; Malepszy, S. . Dep. of Plant Genetics

Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber (Cucumis sativus L.)

1998

Plader, W. | Hoshi, Y. | Malepszy, S. (Warsaw Agricultural University (Poland). Dep. of Plant Genetics)

Each of the seven chromosomes in cucumber was identified using sequential staining with Chromomycin A3 and 4-6-diamidi-no-2-phenylindole as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-meta-phase chromosome complements

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AGROVOC Keywords

adn chromosome chromosomes cromosomas cucumis sativus dna fluorescence fluorescencia haploidia haploidie haploidy inbred lines laboratory experimentation meiose meiosis methode methods mitose mitosis nucleotide sequence

Bibliographic information

Published in

Journal of Applied Genetics (Poland)

Volume 39 Issue 3 ISSN 1234-1983

Pagination

pp. 249-258

Other Subjects

Lignee consanguine; Secuencia nucleotidica; Metodos; Lineas consanguineas; Sequence nucleotidique; Experimentacion en laboratorio; Experimentation en laboratoire

Language

English

Note

Summary (En)

5 fig., 1 table; 19 ref.

Type

Summary

In AGRIS since: 1999-08-15

Format: AGRIS AP

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Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber (Cucumis sativus L.)

1998

AGROVOC Keywords

Bibliographic information