Sol-gel-based enzymatic assays and immunoassays for residue analysis [pesticides - herbicides - nitroaromatic pollutants]

Altstein, M.; Aharonson, N.; Segev, G.; Ben-Aziz, O.; Bronshtein, A. . Inst. of Plant Protection; Avnir, D.; Turniansky, A. . Inst. of Chemistry

Sol-gel-based enzymatic assays and immunoassays for residue analysis [pesticides - herbicides - nitroaromatic pollutants]

2000

English. A novel technology based on the entrapment of biomolecules in a ceramic SiO2 sol-gel matrix has been developed. The technology was used to entrap enzymes and antibodies for use as pesticide and pollutant sensors and immunochromatographic materials. Three systems were studied: (i) sol-gel-entrapped esterases for monitoring organophosphate (OP) and carbamate (CB) pesticides; (ii) sol-gel-entrapped anti-atrazine monoclonal antibodies for immuno-affinity purification of s-triazine herbicides present as contaminants in soil and water; and (iii) sol-gel-entrapped anti-dinitrophenyl (DNP) polyclonal antiserum for clean up and concentration of nitroaromatic pollutants from agricultural and environmental samples. The characteristics of the sol-gel-entrapped enzymes and antibodies are presented

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Italian. E' stata messa a punto una nuova tecnologia basata sull'intrappolamento di biomolecole su una matrice silicea sol-gel. La tecnologia e' stata utilizzata per intrappolare enzimi ed anticorpi utilizzabili come pesticidi, come sensori di inquinamento e come materiali per immunocromatografia. Sono stati studiati i seguenti 3 sistemi di intrappolamento sol-gel: (i) esterasi per rivelazione di organofosfati (OP), carbammati (CB) e pesticidi; (ii) anticorpi monoclonali anti-atrazina intrappolati in sol-gel per purificazione mediante immuno-affinita' di erbicidi a base di s-triazina, presenti come contaminanti nei suoli e nelle acque; (iii) antisieri policlonali di anti-dinitrofenili (DNP) per pulizia e concentrazione di contaminanti nitroaromatici da campioni agricoli ed ambientali. Sono infine proposte le caratteristiche degli enzimi e degli anticorpi intrappolati in sol-gel

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AGROVOC Keywords

antibodies anticorpi anticorps anticuerpos atrazina atrazine biologia molecolare biologia molecular carbamate carbamates carbamatos carbammati chemical contamination chromatographie chromatography contamination chimique contaminazione chimica control de calidad cromatografia environmental impact enzimas enzimi enzyme enzymes immunologia immunological techniques immunologie immunology impact sur l'environnement impacto ambiental impatto ambientale molecular biology persistance des pesticides persistencia de los plaguicidas pesticide pesticide persistence pesticides plaguicidas residues residui residuos technique immunologique tecniche immunologiche

Bibliographic information

Published in

Italian Journal of Food Science (Italy)

Volume 12 Issue 2 ISSN 1120-1770

Pagination

pp. 191-206

Other Subjects

Persistenza dei pesticidi; Residu; Biologie moleculaire; Controle de qualite; Quality controls; Contaminacion quimica; Pesticidi; Tecnicas inmunologicas; Controlli di qualita'; Inmunologia

Language

English

Note

Summaries (En, It)

6 tables; 4 graphs; 50 ref.

Translated Title

Analisi di residui mediante test enzimatici ed immunologici basati su intrappolamento sol-gel [antiparassitari - erbicidi - inquinanti nitroaromatici]

Type

Summary; Bibliography

In AGRIS since: 2001-08-15

Format: AGRIS AP

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Sol-gel-based enzymatic assays and immunoassays for residue analysis [pesticides - herbicides - nitroaromatic pollutants]

2000

AGROVOC Keywords

Bibliographic information