Sol-gel-based enzymatic assays and immunoassays for residue analysis [pesticides - herbicides - nitroaromatic pollutants]
2000
Altstein, M. | Aharonson, N. | Segev, G. | Ben-Aziz, O. | Bronshtein, A. (Volcani Center, Bet Dagan (Israel). Inst. of Plant Protection) | Avnir, D. | Turniansky, A. (Hebrew Univ., Jerusalem (Israel). Inst. of Chemistry)
Английский. A novel technology based on the entrapment of biomolecules in a ceramic SiO2 sol-gel matrix has been developed. The technology was used to entrap enzymes and antibodies for use as pesticide and pollutant sensors and immunochromatographic materials. Three systems were studied: (i) sol-gel-entrapped esterases for monitoring organophosphate (OP) and carbamate (CB) pesticides; (ii) sol-gel-entrapped anti-atrazine monoclonal antibodies for immuno-affinity purification of s-triazine herbicides present as contaminants in soil and water; and (iii) sol-gel-entrapped anti-dinitrophenyl (DNP) polyclonal antiserum for clean up and concentration of nitroaromatic pollutants from agricultural and environmental samples. The characteristics of the sol-gel-entrapped enzymes and antibodies are presented
Показать больше [+] Меньше [-]итальянский. E' stata messa a punto una nuova tecnologia basata sull'intrappolamento di biomolecole su una matrice silicea sol-gel. La tecnologia e' stata utilizzata per intrappolare enzimi ed anticorpi utilizzabili come pesticidi, come sensori di inquinamento e come materiali per immunocromatografia. Sono stati studiati i seguenti 3 sistemi di intrappolamento sol-gel: (i) esterasi per rivelazione di organofosfati (OP), carbammati (CB) e pesticidi; (ii) anticorpi monoclonali anti-atrazina intrappolati in sol-gel per purificazione mediante immuno-affinita' di erbicidi a base di s-triazina, presenti come contaminanti nei suoli e nelle acque; (iii) antisieri policlonali di anti-dinitrofenili (DNP) per pulizia e concentrazione di contaminanti nitroaromatici da campioni agricoli ed ambientali. Sono infine proposte le caratteristiche degli enzimi e degli anticorpi intrappolati in sol-gel
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