High molecular weight glutenin subunits composition of Syrian grown bread wheat and its relationships with gluten strength [Triticum aestivum L.]
1999
MirAli, N. | Arabi, M.I.E. | Al-Safadi, B. (Atomic Energy Commission of Syria, Damascus. Dept. of Agriculture)
English. The high molecular weight composition of glutenin subunits (HMW-GS) of 99 Syrian grown bread wheat genotypes was studied using 10% polyacrylamid gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). Twelve different HMW-GS were detected with 4 or 5 subunits present in any genotype. The results indicated that these genotypes contain different allelic frequencies to those reported by other workers at all three loci. Glu-D1 was the most important locus with 5+10 having the highest value, followed by Glu-B1 with 13+16 and 17+18 subunits as the most important alleles. Glu-Al alleles, however, had, in general, no influence on SDS sedimentation values. The relationships between the different alleles and the impact of each locus on gluten strength as determined by SDS sedimentation values are discussed
Show more [+] Less [-]Italian. [E' stata studiata la composizione delle subunita' di glutenine ad alto peso molecolare (HMW-GS) di 99 genotipi di frumento tenero coltivati in Siria, utilizzando l'elettroforesi in gel di poliacrilamide al 10% in presenza di sodio dodecil solfato (SDS-PAGE). Sono state individuate dodici diverse HMW-GS con 4 o 5 subunita' in ogni genotipo. I risultati indicavano che questi genotipi contengono frequenze alleliche diverse rispetto a quelle riportate da altri ricercatori ai tre loci. Glu-D1 era il locus piu' importante, con il valore piu' elevato di 5+10, seguito da Glu-B1 con le subunita' 13+16 e 17+18 come alleli piu' importanti. Sono discussi i rapporti fra i diversi alleli e l'impatto di ogni locus sulla forza del glutine determinata mediante i valori di sedimentazione SDS]
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