Cloning and characterization of a genomic probe for malignant catarrhal fever virus

Shih, L.M.; Irving, J.M.; Zee, Y.C.; Pritchett, R.F.

Cloning and characterization of a genomic probe for malignant catarrhal fever virus

1988

Shih, L.M. | Irving, J.M. | Zee, Y.C. | Pritchett, R.F.

A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.

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AGROVOC Keywords

analysis animals cattle cattle cloning cloning dna dna probes genes genetics herpesviridae microbiology nucleic acid hybridization sheep viruses

Bibliographic information

Published in

American journal of veterinary research

Volume 49 Issue 10 Pagination 1665 - 1668 ISSN 0002-9645

Publisher

Elsevier B.V.

Other Subjects

Malignant catarrh; Molecular; Characterization; Malignant catarrhal fever; Probes (equipment); Viral; Isolation & purification; Blotting; Southern; Dna restriction enzymes; Viral

Language

English

Type

Text; Journal Article

In AGRIS since: 2024-02-27

Format: MODS

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