Cloning and characterization of a genomic probe for malignant catarrhal fever virus

Shih, L.M.; Irving, J.M.; Zee, Y.C.; Pritchett, R.F.

Cloning and characterization of a genomic probe for malignant catarrhal fever virus

1988

Shih, L.M. | Irving, J.M. | Zee, Y.C. | Pritchett, R.F.

A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.

Показать больше [+]

Ключевые слова АГРОВОК

analysis animals cattle cattle cloning cloning dna dna probes genes genetics herpesviridae microbiology nucleic acid hybridization sheep viruses

Библиографическая информация

Опубликовано в

American journal of veterinary research

Том 49 Выпуск 10 Нумерация страниц 1665 - 1668 ISSN 0002-9645

Издатель

Elsevier B.V.

Другие темы

Malignant catarrh; Molecular; Characterization; Malignant catarrhal fever; Probes (equipment); Viral; Isolation & purification; Blotting; Southern; Dna restriction enzymes; Viral

Язык

Английский

Тип

Text; Journal Article

В АГРИСе с: 2024-02-27

Формат: MODS

Поставщик данных

Эту запись предоставил National Agricultural Library

Откройте коллекцию этого поставщика данных в AGRIS

Посмотрите в Google Scholar

If you notice any incorrect information relating to this record, please contact us at agris@fao.org agris@fao.org