Oligonucleotide probes detect splicing variants in situ in Drosophila embryos

Artero, R.D.; Akam, M.; Perez-Alonso, M.

Oligonucleotide probes detect splicing variants in situ in Drosophila embryos

1992

Artero, R.D. | Akam, M. | Perez-Alonso, M.

We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and IIa are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.

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AGROVOC Keywords

animals digoxigenin dna probes drosophila drosophila melanogaster embryogenesis genes messenger rna methods nucleic acid hybridization transcription

Bibliographic information

Published in

Nucleic acids research

Volume 20 Issue 21 Pagination 5687 - 5690 ISSN 0305-1048

Publisher

Kluwer Academic Publishers

Other Subjects

Alternative splicing; Antisense dna; Transcription (genetics); Ubx gene; Alternative splicing; Genetic; Rna editing; Embryo (animal); In situ hybridization; Antisense; Oligonucleotides; Transcripts; Oligonucleotide probes; Base sequence; Antisense oligonucleotides; Molecular sequence data

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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Oligonucleotide probes detect splicing variants in situ in Drosophila embryos

1992

AGROVOC Keywords

Bibliographic information