Oligonucleotide probes detect splicing variants in situ in Drosophila embryos
1992
Artero, R.D. | Akam, M. | Perez-Alonso, M.
We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and IIa are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.
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书目信息
Nucleic acids research
卷
20
期
21
页码
5687
- 5690
ISSN
0305-1048
出版者
Kluwer Academic Publishers
其它主题
Antisense dna; Ubx gene; Base sequence; In situ hybridization; Embryo (animal); Antisense; Oligonucleotides; Rna editing; Oligonucleotide probes; Transcripts; Transcription (genetics); Alternative splicing; Alternative splicing; Antisense oligonucleotides; Molecular sequence data; Genetic
语言
英语
类型
Journal Article; Text
2024-02-28
MODS