Oligonucleotide probes detect splicing variants in situ in Drosophila embryos
1992
Artero, R.D. | Akam, M. | Perez-Alonso, M.
We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and IIa are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.
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书目信息
出版者
Kluwer Academic Publishers
其它主题
Alternative splicing; Antisense dna; Transcription (genetics); Ubx gene; Alternative splicing; Genetic; Rna editing; Embryo (animal); In situ hybridization; Antisense; Oligonucleotides; Transcripts; Oligonucleotide probes; Base sequence; Antisense oligonucleotides; Molecular sequence data
语言
英语
类型
Journal Article; Text
2024-02-28
MODS