Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range
2022
Ryoya Nakagawa | Soh Ishiguro | Sae Okazaki | Hideto Mori | Mamoru Tanaka | Hiroyuki Aburatani | Nozomu Yachie | Hiroshi Nishimasu | Osamu Nureki
SpCas9 is a versatile genome-editing tool, but it is large and cannot be packaged efficiently in a AAV vector, limiting its application. The reported engineered Campylobacter jejuni Cas9 variant exhibits enhanced cleavage activity and a broader targeting range, expanding the CRISPR-Cas toolbox for therapeutic genome engineering.
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