Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range

Ryoya Nakagawa; Soh Ishiguro; Sae Okazaki; Hideto Mori; Mamoru Tanaka; Hiroyuki Aburatani; Nozomu Yachie; Hiroshi Nishimasu; Osamu Nureki

Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range

2022

SpCas9 is a versatile genome-editing tool, but it is large and cannot be packaged efficiently in a AAV vector, limiting its application. The reported engineered Campylobacter jejuni Cas9 variant exhibits enhanced cleavage activity and a broader targeting range, expanding the CRISPR-Cas toolbox for therapeutic genome engineering.

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