EU-OSTID: A collection of transposon insertional mutants for functional genomics in rice
2005
Enckevort, L. (Ellen) J. G van | Droc, Gaëtan | Piffanelli, Pietro | Greco, Raffaella | Gagneur, Cyril | Weber, Christele | González, Víctor M. | Cabot, Pere | Fornara, Fabio | Berri, Stefano | Miro, Berta | Lan, Ping | Rafel, Marta | Capell, Teresa | Puigdomènech, Pere | Ouwerkerk, Pieter B.F. | Meijer, Annemarie H. | Pe’, Enrico | Colombo, Lucia | Christou, Paul | Guiderdoni, Emmanuel | Pereira, Andy | European Commission
A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.
Show more [+] Less [-]We acknowledge the support of the EU 5th framework project QLG2-CT-2001-01453 CerealGene Tags.
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