EU-OSTID: A collection of transposon insertional mutants for functional genomics in rice

Enckevort, L.  J. G van; Droc, Gaëtan; Piffanelli, Pietro; Greco, Raffaella; Gagneur, Cyril; Weber, Christele; González, Víctor M.; Cabot, Pere; Fornara, Fabio; Berri, Stefano; Miro, Berta; Lan, Ping; Rafel, Marta; Capell, Teresa; Puigdomènech, Pere; Ouwerkerk, Pieter B.F.; Meijer, Annemarie H.; Pe’, Enrico; Colombo, Lucia; Christou, Paul; Guiderdoni, Emmanuel; Pereira, Andy; European Commission

EU-OSTID: A collection of transposon insertional mutants for functional genomics in rice

2005

A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.

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We acknowledge the support of the EU 5th framework project QLG2-CT-2001-01453 CerealGene Tags.

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Peer reviewed

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