Membrane fractionation of hydrolysates of the water-soluble protein from tuna fish meal obtained by subcritical water and enzymatic treatments. Comparison of physical and chemical properties
2025
Barea Gómez, Pedro | Illera Gigante, Alba Ester | Candela Gil, Helena | Melgosa Gómez, Rodrigo | Benito Moreno, José Manuel | Beltrán Calvo, Sagrario | Sanz Díez, Mª Teresa
Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO2 (subW-CO2) and Alcalase® treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO2 hydrolysates with polyamide membranes (800–600 Da and 300–150 Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase® hydrolysates were fractionated by ultrafiltration (UF, 10 kDa) followed by NF (1–1.1 kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A relatively wide molecular weight distribution was observed in all streams, with high antioxidant and iron-chelating capacities in the retentate fractions.
Show more [+] Less [-]This work was supported by the Agencia Estatal de Investigación [Grant numbers PID2022-136385OB-I00, PDC2022-133443-I00] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [Grant number BU027P23]. P. Barea pre-doctoral contract was funded by JCyL and the European Social Fund (ESF) [ORDEN EDU/1868/2022]. A. E. Illera postdoctoral contract was funded by BU027P23. H. Candela-Gil contract was funded by PDC2022-133443-I00. R. Melgosa contract was funded by a Beatriz Galindo Research Fellowship [BG20/00182].
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