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Phenoloxidase activity of micromycetes strains isolated from the rhizosphere of vegetable leguminous crops
2019
Yu. N. Kurkina
Relevance. Microscopic fungi exhibit the greatest exoenzymatic activity, and xylotrophs are capable of possessing a complex of oxidase and hydrolase enzymes to destroy lignin.Methods. Among the soil micromycetes isolated from the rhizosphere of annual leguminous crops, a search was made for strains that are promising from the point of view of effective microorganism technology, in order to create optimal conditions for increasing soil fertility and crop yields. The detection of total phenol oxidase activity was carried out according to the method of Bavendamm, cultivating the strains on microbiological agar with the addition of an aromatic lignin derivative (0.06% tannin) and the growth factor was calculated. The cellulase activity of the strains was judged by the degree of hydrolysis of the filter paper. Alternaria alternata, Bipolaris, Botrytis fabae, Cunninghamella echinulata, Curvularia lunata, Macrophoma phaseolicola, Trichoderma koningii, Trichoderma viride and Ulocladium botrytis strains were found to cleave lignin and cellulose.Results. When comparing the phenoloxidase activity of the strains, it was proposed to take into account the growth factor, expressed as the ratio of the diameter of the colony on the substrate with the addition of tannin to the diameter of the colony without tannin. Thus, perspective strains of T. koningii and U. botrytis strains with respect to the complex activity of enzymes were identified. The strains C. lunata, A. alternata and B. australiensis showed high cellulase activity. The strain of non-pathogenic soil fungus C. echinulata has the ability to degrade lignin
Show more [+] Less [-]MAIN DIRECTIONS AND RESULTS OF VEGETABLE BREEDING AND SEED PRODUCTION OF VEGETABLE CROPS OF LEGUMES IN VNIISSOK
2015
V. F. Pivovarov | E. P. Pronina
The paper demonstrates the basic achievements in breeding and seed production of vegetable legumes in All,Russian Research Institute of vegetable breeding and seed production and the directions of the development of advanced varieties, which are very important at this stage.
Show more [+] Less [-]VARIABILITY OF THE BIOCHEMICAL COMPOSITION OF GREEN^PODDED OF COMMON BEAN
2015
T. K. Gorovaya | O. Yu. Sayko
The results of study of 21 collection samples for variability of the biochemical composition of green beans are presented. The best selected samples characterized by stable content of total sugars, dry matter, and diet fiber are recommended for use in breeding program for development of new improved varieties of vegetable beans.
Show more [+] Less [-]THE INITIAL MATERIAL FOR GRAIN LEGUMES BREEDING IN THE COLLECTION OF VIR
2015
M. A. Vishnjakova | S. V. Bulintsev | M. O. Burlyaeva | T. V. Buravtseva | G. P. Egorova | E. V. Semenova | I. V. Seferova
In the VIR collection there is a rich initial material, which can contribute to the intensification of breeding of leguminous of vegetable directions of our country, as well as an increase in speciesdiversity of cultures in this group.
Show more [+] Less [-]BEAN CULTURE IN CHERNOZEM ZONE OF RUSSIA
2015
I. T. Balashova | E. P. Pronina | S. M. Sirota | D. K. Gordeev
Beans (Vicia faba L.) is the one of the ancient crops which have been cultivated and used for food. The historical note about bean utilization in ancient world and in Russia, and the information aboutcenters of origin, food value of seeds are presented in this review. Botanical characteristics of three bean varieties of VNIISSOK breeding are described.
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