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Influence of varietal characteristics the cabbage in the quality of the products used for pickling after a long storage period
2019
V. I. Tereshonok | M. Yu. Markarova | N. E. Posokina | L. L. Bondareva | S. M. Nadezhkin
Relevance. Fermentation of cabbage is a common way of processing, which allows to preserve and strengthen its useful qualities. To date, breeders have proposed a significant number of varieties and hybrids of cabbage, each of which has its own individual biochemical characteristics. Traditional sauerkraut is carried out after harvest. Long storage can affect the change in the content of organic acids and sugars in cabbage.Methods. The aim of the work was to study the parameters of the fermentation process of eight varieties and hybrids of cabbage by selection in the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center by the intensity of sugar reduction, accumulation of organic acids, organoleptic parameters and activity of lactic acid microorganisms. Fermentation was carried out after a 5-month period of storage of heads.Results. The results showed that the storage period did not affect the fermentation result of most of the studied varieties and hybrids of cabbage. There are varietal differences in the initial content of mono-and disaccharides, organic acids, which affected the fermentation process. The increased content of malic acid in the variety population Podarok, F1 Severyanka contributed to the slowdown of the first stage of fermentation. Varietal features of the Parus population contributed to the slowdown of the first and second stages of fermentation, which was manifested in a decrease in the rate of destruction of sugars. Variety Zymovka 1474 in the process of processing by fermentation accumulates a significant amount of succinic acid, which can characterize its special difference among the studied varieties and hybrids. The best organoleptic characteristics were distinguished by hybrids of fermented cabbage F1 Likova, F1 Natali, F1 Mechta and F1 Snezhinka. The results of the study suggest that the processing of cabbage by fermentation should take into account its individual varietal biochemical features. This will not only preserve the valuable nutritional properties of the cabbage itself, but also strengthen them by optimizing the natural process of lactic acid fermentation. The result will be the best organoleptic effect achieved by an optimal combination of lactic and citric acids and mono-sugars.
Show more [+] Less [-]Dry fermented vegetable products of long-term storage and their integration into the diet of astronauts
2024
E. V. Yanchenko | K. A. Zykin | N. E. Kaukhcheshvili | A. A. Gryzunov
Relevance. Fermentation makes it possible to improve the properties of the final product, increase its nutritional value and organoleptic characteristics. It has been scientifically proven that the use of fermented foods (with high nutritional and biological value) on a regular basis has a positive effect on human health, increases immunity, improves the functioning of the gastrointestinal tract. Dry fermented products with a long shelf life can be recommended as part of the diet in space. The aim is to creation of a dry base for fermented long-term storage beverages based on cabbage and table carrots, applicable in the diet of space nutrition. Objects and methods of research. The objects of the study were the domestic hybrid of white cabbage F1 Severyanka and the domestic variety of carrot table Margosha, and the dry fermented products obtained from them. Results. It was found that, according to biochemical and organoleptic quality indicators, the domestic hybrid of white cabbage F1 Severyanka and the domestic carrot variety Margosha are suitable for various types of processing, including fermentation and manufacture of dried fermented products. The comparison of the number of microorganisms in fermented cabbage and cabbage dehydrated by LTVD (low temperature vacuum drying) and VFD (vacuum freeze drying methods was carried out. The survival rate of microorganisms was 1.62 times higher in LTVD. The ways of accelerating the fermentation process of table carrots are considered. The expediency of using starter cultures obtained on the basis of the fermented product has been confirmed. It has been found that using starter cultures in the form of a fermented product dried by the LTVD method accelerates fermentation and allows you to reach the desired pH level 1.33 times faster than the base one and does not affect the final organoleptic characteristics.
Show more [+] Less [-]METHOD OF DETERMINING THE DYNAMICS OF INTERACTION LACTIC ACID BACTERIA IN THEIR JOINT CULTIVATION
2018
V. V. Kondratenko | N. E. Posokina | O. Yu. Lyalina | A. Yu. Gracheva | Zh. A. Semenova | V. I. Tereshonok
The processes of fermentation, accompanied by the growth and development of several species of microorganisms, are often accompanied by a change of some species of microorganisms for anothers , such a complex "behavior" of microorganisms during fermentation needs to be be deeply researched. The previously used approaches of evaluation of the type of interaction of particulate monocultures in consortiums make it possible to obtain only qualitative results (synergistic, antagonistic, additive interaction) generally, without taking into the consideration the change of this interaction during the cultivation. The purpose of the research was the development of a new approach of the quantitative determination of the parameter of the interaction of microorganisms of one species with another species expressed as a continuous function determined throughout the all fermentation period. In the process of the work a two-component consortium of lactic acid microorganisms Lactobacillus brevis and Lactobacillus plantarum was selected, cultivated in two different model media as part of a study of directed fermentation of vegetable products. The work the cultivation of monocultures (included in the studied consortium) was also carried out. The performed work the original approach was developed, the approach is based on a comparison of the growth rate of the microorganisms biomass of the consortium with the calculated additive dynamics. This approach is convenient to use to identify a complex regularity of changes of the types of interaction of microorganisms in the consortium in the form of a continuous function determined during the entire cultivation period. From the point of view of the synergies of the species of microorganisms entering the studied consortium in the active phase of fermentation, the "Slava" variety turned to be more profitable than the "Parus".
Show more [+] Less [-]Influence of carbohydrate correction of raw materials on the growth of lactic acid microorganisms in the process of directed fermentation of vegetables
2020
J. A. Semenova | N. E. Posokina | V. I. Tereshonok
Relevance. The use of certain single or mixed cultures of lactic acid microorganisms and the mandatory control of the fermentation process of white cabbage are of great importance to ensure that the product is of stable quality without the need for any chemical preservatives or harsh processing conditions. Carbohydrates contained in raw materials play an important role in this process, since carbohydrates are the main source of maintaining the viability of lactic acid microorganisms. By adjusting the carbohydrate component of raw materials at different stages of fermentation, it is possible to maintain a high titer of microorganisms, while obtaining not only a high-quality product, but also giving it, along with prebiotic (a source of fiber) and probiotic properties. Materials and methods. The purpose of this research work was to ensure the direction of the biotechnological process in the direction of stabilization of the concentration of functional microflora (starting systems of lactic acid microorganisms), under the influence of introduced carbohydrate nutrients. In this work, we studied two fermentation systems of microorganisms that are fundamental microorganisms in the fermentation of cabbage, namely, systems consisting of a pre-fermentation culture of Leuconostoc mesenteroides and one of the cultures of lactobacilli: Lactobacillus plantarum or Lactobacillus brevis. The study used the usual microbiological method for determining the number of microorganisms by seeding in an agarized culture medium, as well as processing experimental data. Results. As a result, we found that the introduction of a carbohydrate Supplement allows us to stabilize the dynamics of changes in the concentration of the studied starting systems at the same initial level throughout the main stage of fermentation.
Show more [+] Less [-]Comparative evaluation of several Dynamics of accumulation of lactic and acetic acids in the process of directed fermentation of white cabbage of the Parus variety
2020
V. V. Kondratenko | N. E. Posokina
Relevance and methodology. Today, white cabbage is the most popular type of vegetable raw materials used for fermentation not only in Russia, but also in other countries. To obtain a variety of flavors and shades of flavor, various vegetables (carrots, sweet peppers), fruit (apples, cranberries) and spicy-aromatic additives (cumin, dill seed, etc.) are added to the cabbage during fermentation. During fermentation, the product contains not only the initial components of the raw material (vitamin C, macro-and micronutrients), but also significantly increases the number of lactic acid microorganisms – the main "participants" in the process.One of the main products of the metabolism of lactic acid microorganisms is lactic acid. At a concentration of 0.5%, it begins to inhibit the development of many microorganisms. When reaching a concentration of 1-2% microbiological enzymatic hydrolysis, as a rule, ceases. The accumulation of acetic acid allows us to judge the intensity of the heterofermentative stage of fermentation using strains of lactic acid microorganisms.Results. The L. plantarum + L. casei consortium has shown a synergistic interaction in the accumulation of lactic acid, since the amount of acid produced by the consortium significantly exceeds the amount of acid produced by each strain separately. This point is also noted for the accumulation of acetic acid. In the L. brevis + L. casei consortium, the opposite situation is observed: when using cultures separately, the accumulation of lactic acid occurs to a greater extent than when using two cultures simultaneously. There are no obvious differences between monocultures and the consortium in terms of acetic acid accumulation. In the case of the L. plantarum + L. brevis consortium, we observe a synergistic interaction, with the dominant role in this consortium played by L. plantarum, and the contribution of L. brevis to the accumulation of lactic acid is insignificant. At the same time, the total resulting accumulation of acetic acid is significantly less than the result of each strain separately, which may indicate a negative mutual effect of the participants in the process. Acid degradation of the polysaccharide matrix of cell walls does not occur during the entire fermentation process.
Show more [+] Less [-]TEROIDES THE STUDY OF THE EFFECT OF HIGH AND LOW STARTING TITRE IN THE MODEL ENVIRONMENTS TO CHANGES IN ACTIVE AND TITRATABLE ACIDITY DURING THE CULTIVATION OF WHITE CABBAGE VARIETIES PARUS LACTIC ACID BACTERIA L. LACTIS AND L. MESENTEROIDES
2018
V. V. Kondratenko | O. Yu. Lyalina | N. E. Posokina | E. S. Shishlova | A. I. Zakharova | V. I. Tereshonok
The aim of the research was to study the dynamics of active and titratable acidity with a low and high initial titer, while cultivating white head cabbage of the variety ‘Parus’ with lactic acid bacteria Leuconostoc lactis VKPM V-12150 and Leuconostoc mesenteroides subsp. mesenteroides VKPM V-8818 in the basic model medium (BMS) and the modified model medium (MMC) during fermentation . In this experiment, two important questions were considered: the creation of favorable conditions for the growth of lactic acid microorganisms and the suppression of undesirable pathogenic microflora, which can eventually lead to product spoilage. One of the key questions is to scale the results of studies related to the fermentation of plant objects by microorganisms on initially non-sterile raw materials that is the requirement to create as possible fast the conditions that restrain the reproduction of Cl. botulinum, preventing toxin accumulation in the product. Therefore, an important factor is the observance for the necessary boundary condition where for international standards, it reaches pH ≤ 4.6, and for Russian standards it reaches pH ≤ 4.2. Hence, an analysis of the experimental data showed that these boundary conditions were reached after the expiration of essentially different time intervals during the cultivation of microorganisms with different starting titers. As a result, it was shown with which lactic acid microorganisms the optimal parameters for the dynamics of active and titratable acidity were obtained, when the boundary condition reached pH 4.2. Since this value determines more "stringent" requirements for product safety. As a result of studies it was found that optimal parameters for the dynamics of active and titratable acidity when a limit pH value of 4.2 is obtained in the cultivation of lactic acid bacteria L. mesenteroides in the MMC, revealing that the value of the starting titer ranging from 3*103 to 3*105 CFU/g.
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