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Characterisation of the QM gene of Trypanosoma brucei Full text
2002
Lillico, Simon G. | Mottram, J.C. | Murphy, N.B. | Welburn, S.C.
The QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of Trypanosoma brucei and determined its sequence. Alignment with QM sequences from Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster and Homo sapiens revealed greater than 60% identity. Southern blot analysis revealed multiple copies of QM within the trypanosome genome. An epitope tag was inserted into the C-terminus of the T. brucei QM and the protein expressed under inducible control in procyclic form trypanosomes. Immune fluorescence microscopy revealed co-localisation with the GPI:protein transamidase component, GPI8, a distribution indicative of ribosome association with the rough endoplasmic reticulum.
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1991
Drent W.J. | Gottschal J.C.
A phylogenetic comparison of the 16S rRNA sequence of the fish pathogen, Renibacterium salmoninarum, to Gram-positive bacteria.
1991
Gutenberger S.K. | Giovannoni S.J. | Field K.G. | Fryer J.L. | Rohovec J.S.
Activity of different 'killer' yeasts on strains of yeast species undesirable in the food industry.
1991
Palpacelli V. | Ciani M. | Rosini G.
Synthetic oligodeoxyribonucleotide probes to detect verocytotoxin-producing Escherichia coli in diseased pigs.
1989
Meyer T. | Bitzan M. | Sandkamp O. | Karch H.
Pseudomonas tolaasii and tolaasin: comparison of symptom induction on a wide range of Agaricus bisporus strains.
1996
Moquet F. | Mamoun M. | Olivier J.M.
Pulsed-field gel electrophoresis for sub-specific differentiation of Serpulina pilosicoli (formerly 'Anguillina coli').
1996
Atyeo R.F. | Oxberry S.L. | Hampson D.J.
Insertion sequence analysis of protoplast fused strains of Lactococcus lactis ssp. cremoris.
1993
Ward L.J.H. | Brown J.C.S. | Davey G.P.
The nucleotide sequence of the gene encoding the A88ab protein subunit of porcine enterotoxigenic Escherichia coli.
1981
Gaastra W. | Mooi F.R. | Stuitje A.R. | Graaf F.K. de
Degradation of gallic acid by a Pseudomonas species.
1980
Trevors J.T. | Basaraba J.