The heat shock protein (HSP) family is one of the proteins universally accumulated under heat stress condition. Four Egyptian maize inbred lines (Zea mays L.) were studied to identify heat shock proteins under heat stress at 45°C as protein markers for detecting the thermo-tolerance inbreds. The protein fingerprints of four inbred lines were performed by grain total-soluble protein electcophoretic analysis. The protein electrophoretic analysis showed 18 bands in a distinct pattern of K1 and K7 inbred lines, while 17 bands were present in G342 and Rg59 inbred lines as another distinct pattern indicating different genotypes. The high temperature effect on four maize inbred lines exposed to 45°C for 2 and 4 hours at 14-days old seedlings besides control (25°C) was studied. Several protein bands varied between low and intermediate molecular weights were induced after exposing to heat stress at 45°C. Four bands of heat shock proteins with molecular weights of 82, 22, 17 and 10 kDa appeared in the inbred line K1 after exposing to 45°C for 2 and 4 hours which may be indication of thermo-tolerance. Four and seven bands were enhanced after exposing to high temperature for 4 hours at 45°C which appeared more concentrated in the patterns of K7 and G342 inbred lines, respectively.

A collection of 12 varieties of broad bean (Vicia faba L.) was studied. This was curried out to see the variation of phenolic compounds (tannins) and proteins of the seeds of Vicia faba L.   This study gave the following results - At  the  biochemical level; according to our results one observed a high content in proteins within the species of Vicia faba L.   - The electrophoresis of total proteins confirmed the richness of proteins of Vicia faba L [albumins (67 kDa), Globulins, Vicilline (50 kDa)]. - Finally the phytochemical analysis (phenolic compounds) allowed us to confirm the richness of the colored seeds in phenolic compounds (condensed tannins) "949 T (183±9.66), Aquadulce (132.19±1.53)". The effect of time (the factor of the environment) inflates on the coloring of the seeds (oxidation) and on the polymerization of tannins.