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SELENIUM INDUCES ANTIOXIDANT DEFENSIVE ENZYMES AND PROMOTES TOLERANCE AGAINST SALINITY STRESS IN CUCUMBER SEEDLINGS (Cucumis sativus) Full text
2010
Se, Selenium; ROS, Reactive oxygen species; APX, Ascorbate peroxidase; CAT, Catalase; PAL, Phenylalanine ammonia-lyase; EDTA, Ethylenediamine tetra acetic acid; MDA, Malondialdehyde; POD, Peroxidase; PVP, Polyvinylpyrrolidone; SOD, Superoxide dismutase; TBA, Thiobarbituric acid; TCA, Trichloroacetic acid; NBT, Nitroblue tetrazolium; P5CR, ∆1-Pyrroline-5-carboxylate reductase; P5CS, ∆1-Pyrroline-5-carboxylate synthetase. OAT, Ornithine-δ-aminotransferase; P5CDH, ∆1-Pyrroline-5-carboxylate dehydrogenase and PDH, Proline dehydrogenase; Poly acrylamide gel electrophoresis (PAGE) ABSTRACT Selenium (Se) is not an essential nutrient for higher plants. Although, it is able to induce stress tolerance in many plant species. The present study was carried out to investigate the ability of Se to promote the tolerance of cucumber seedlings to salinity stress and to identify some biochemical parameter associated with Se–induced resistance in cucumber seedlings. Plants were irrigated with 2000 ppm NaCl after treatment with 1ppm Se for two weeks and samples collected after 7 days from started salt-treatment. The biochemical changes due to treatment with Se and /or NaCl were monitored by determination of lipid peroxidation (LPO), proline, phenolic compounds and percentage of electrolyte leakage (EL) from plant tissues. Moreover, the activities of peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD) and phenylalanine ammonialyase (PAL) were determined. The treatment of cucumber seedling with salinity (2000 ppm NaCl) in the presence or absence of selenium caused a remarkable elevation in free proline concentration and the level of phenolic compounds. Salinity stress caused a high level of lipid peroxidation which led to increase the percentage of electrolyte leakage from plant tissues. Se treatment caused a significant reduction in electrolyte leakage and malondialdehyde concentration of cucumber seedlings. The specific activities of antioxidant enzymes (POD, SOD, and APX) and PAL were significantly increased by the treatment with NaCl. Also, Se caused a significant elevation in the specific activities of POD, CAT, SOD, APX and PAL in cucumber seedlings grown under salt stress condition. On the other hand, Se caused a significant decrease in SOD activity in cucumber seedlings grown in non-salty condition. The isoenzymes pattern of POD demonstrated that peroxidase was up-regulated by Se either in control or NaCl-treated seedlings. The results concluded that Se induce six POD isoenzymes in cucumber seedlings. Se reduces and overcomes the oxidative injury caused by salinity stress. Moreover, the protective effect of Se against salinity stress may be carried out through a mechanism included activation of PAL which is responsible for phenolic compounds biosynthesis. The accumulation of these phenolic compounds in Se-treated seedling leads to a high activities of antioxidant defensive enzymes and increase the tolerance level towards salinity stress.
Show more [+] Less [-]DESIGNING AN INTEGRATED COMPUTER PROGRAM FOR VEGETABLE INSECT, NON INSECT PESTS AND DISEASES AFFECTING VEGETABLE CROPS Full text
2010
Abdulrahman Aldawood | Ibrahim Alshahwan | Abdullah Al-Sadon | Abdulrahman Al-Azba | Omar Abdalla | Alshamy Adam | Ali Almasrahy
The major vegetable crop production in the Kingdom of Saudi Arabia require easily produced sources of related information. However, there is a lack of such studies that lead to the use of com-puter programs or geographical information sys-tems in developing an electronic source for these extension, research, and scientific data. Farmers and agricultural engineers need a comprehensive database regarding insect pests, non insects pests, and diseases infecting vegetable crops in Saudi Arabia in Arabic language. Therefore, this project has been executed to design a complete database of such information. Included in this software are colored photographs, which will help users to get acquainted with methods of manage-ment of these pests and diseases. A data sheet has been designed to collect field information for each vege-table crop, including its pests and dis-eases, through field trips for many farms in all the 13 agricultural regions of Saudi Arabia. This field information has been compiled with what has been published in related scientific references. Upon completion of this program, confirmatory trips for five regions had been made in order to meet with farmers, agricultural engineers, and extension agents who are in agricultural branches of the Min-istry of Agriculture in these visited regions. The objectives of these trips were to get feedback about this program. Finally, an elaborate imple-mentation guide was prepared under the title "De-signing an integrated computer program for vege-table cultivation in The Kingdom of Saudi Arabia." Also, an attached manual, regarding the compo-nents and the operation of this program, was made. All of these materials have been distributed to farmers and interested people in the agricultural sector
Show more [+] Less [-]IMPROVEMENT PROPERTIES OF WHEAT FLOUR USING MICROWAVE Full text
2010
Ayman Soliman
IMPROVEMENT PROPERTIES OF WHEAT FLOUR USING MICROWAVE Full text
2010
Ayman Soliman
In view of balady bread in Egypt is considered as backbone in diets, accordingly, in this study wheat flour (82%) extraction treated by microwave at 2,4 and 6 minutes, that target to modify starch wheat flour by heating. The rheological properties were studied by using farinograph and extensograph apparatus. Falling number and amylose percent were determined. Balady bread samples were sensory evaluated and staling rate was evaluated. The wheat flour, crust and crumb layers of balady bread were examined by scanning electron microscope (SEM). The results of farinograph indicated that, water absorption arrival time, dough development time and dough stability increased while dough weakening decreased in wheat flour treated by microwave compared with wheat flour untreated (control). The results of extensograph revealed that, dough extensibility, resistance to extension and dough energy increased while proportional number decreased in wheat flour treated by microwave compared with control. Falling number decreased while amylose percent increased when microwave treatment increased from 2 to 6 minutes. Sensory evaluation showed slight decrease in values of both color crust and color crumb while, values of both taste and flavor slightly increased but not significantly different, meanwhile, values of crumb distribution significantly increased in balady bread samples prepared from wheat flour treated by microwave compared with balady bread prepared from untreated wheat flour (control). Wheat flour treated by microwave led to delay staling of balady bread and imilorated freshness of balady bread. The examination by scanning electron microscope (SEM) showed that average size of starch granules increased from 26.10 mm in untreated wheat flour (control) to 29.37 mm in treated wheat flour by microwave at 6 minutes.
Show more [+] Less [-]Improvement properties of wheat flour using microwave
2010
Soliman, A.E.M.
\{ In view of balady bread in Egypt is considered as backbone in diets, accordingly, in this study wheat flour (82%) extraction treated by microwave at 2,4 and 6 minutes, that target to modify starch wheat flour by heating. The rheological properties were studied by using farinograph and extensograph apparatus. Falling number and amylose percent were determined. Balady bread samples were sensory evaluated and staling rate was evaluated. The wheat flour, crust and crumb layers of balady bread were examined by scanning electron micro scope (SEM). The results of farinograph indicated that, water absorption arrival time, dough development time and dough stability increased while dough weakening decreased in wheat flour treated by microwave compared with wheat flour untreated (control). The results of extensograph revealed that, dough extensibility, resistance to extension and dough energy increased while proportional number decreased in wheat flour treated by microwave compared with control. Falling number decreased while amylose percent increased when microwave treatment increased from 2 to 6 minutes. Sensory evaluation showed slight decrease in values of both color crust and color crumb while, values of both taste and flavor slightly increased but not significantly different, meanwhile, values of crumb distribution significantly increased in balady bread samples prepared from wheat flour treated by microwave compared with balady bread prepared from untreated wheat flour (control). Wheat flour treated by microwave led to delay staling of balady bread and imilorated freshness of balady bread. The examination by scanning electron microscope (SEM) showed that average size of starch granules increased from 26.10 µm in untreated wheat flour (control) to 29.37 µm in treated wheat flour by microwave at 6 minutes.
Show more [+] Less [-]FORMULATION OF LAYERS RATIONS WITH SPREADSHEET SOFTWARE EXCEL Full text
2010
Khuleel Rafh Mohammed Taher
FORMULATION OF LAYERS RATIONS WITH SPREADSHEET SOFTWARE EXCEL Full text
2010
Khuleel Rafh Mohammed Taher
The aim of this study was to use spreadsheet software (Excel) to solve linear programming models in formulating least cost rations of egg layers. The ingredients used are corn, wheat, soybean meal, barley, wheat bran, protein concentrate, limestone, salt, dicalcuim phosphate, vitamin and mineral premix, vegetable oil. Results showed that the starter optimum ration for layers from (0 – 6) weeks, consist of these ingredients (9.40, 26.91, 16.38, 38.71, 4.51, 0.95, 1.09, 0.25, 1.18, 0.25, 0.33)% respectively and from (6-12) week of (0, 73.43, 8.72, 0, 14.37, 0, 0.99, 0.25, 1.39, 0.25, 0.57) % respectively, from (12-18) week (0, 78.36, 6.17, 0, 12.02, 0, 1.44, 0.25, 0.87, 0.25, 0.61)% respectively, from (18 week - start of production) (0, 75.55, 13.85, 0, 3.81, 0, 4.25, 0.25, 1.16, 0.25, 0.86 )% respectively , and during productive period from (0, 66.93, 10.62, 0,0, 9.09,9.01, 0.25, 3.82)% respectively, and the cost of these ration was 160.6, 130.70, 123.60, 145.22, 195.95) Iraqi Dinar/kg respectively. Results also show sensitivity report of allowable increase and decrease in ingredient cost and in constraint values and the shadow price.
Show more [+] Less [-]Formulation of layers rations with spreadsheet software excel
2010
Khuleel, R.M.T.
The aim of this study was to use spreadsheet software (Excel) to solve linear programming models in formulating least cost rations of egg layers. The Ingredients used are corn, wheat, soybean meal, barley, wheat bran, protein concentrate, limestone, salt, dicalcuim phosphate, vitamin and mineral premix, vegetable oil. Results showed that the starter optimum ration for layers from (0 - 6) weeks, consist of these Ingredients (9.40, 26.91, 16.38, 38.71,' 4.51, 0.95, 1.09, 0.25, 1.18, 0.25, (Received April 18, 2009) (Accepted September 28, 2009) 0.33)% respectively and from (6-12) week of (0, 73.43, 8.72, 0, 14.37, 0, 0.99, 0.25, 1.39, 0.25, 0.57) % respectively, from (12-18) week (0, 78.36, 6.17, 0, 12.02, 0, 1.44, 0.25, 0.87, 0.25, 0.61)% respectively, from (18 week - start of production) (0, 15.55, 13.85, 0, 3.81, 0, 4.25, 0.25, 1.16, 0.25, 0.86 )% respectively, and during productive period from (0, 66.93, 10.62, 0,0, 9.09,9.01, 0.25, 3.82)% respectively, and the cost of these ration was 160.6, 130.70, 123.60, 145.22, 195.95) Iraqi Dinar/kg respectively. Results also show sensitivity report of allowable increase and decrease in ingredient cost and in constraint values and the shadow price.
Show more [+] Less [-]EFFECT OF HEAT TREATMENT ON THE NUTRITIVE VALUE AND RESIDUES OF SOME SYNTHETIC PESTICIDES IN FRESH BOLTI FISH Full text
2010
Khaled Al-Joumaa
Fresh Bolti fish (Tilpia nilotica) collected randomly from 9 different markets in Ismailia Governorate for evaluation the effect of grilled fish by the method used in grillrooms and houses on the concentration of pesticide residues found and the nutritive value. Results revealed that decrease in the estimated parameters i.e. moisture, crude protein, fat and ash by grilling, this decrease were 6.07, 2.63, 4.07 and 1.56%, respectively. On the contrary, carbohydrates behaved another behavior that there was an obvious increase ranged from 1.98% to 2.92%. Fresh and grilled fish were analyzed to detect 12 organochlorine (OC) and 7 synthetic pyrethroid (SP) pesticides with a mean level on a lipid basis. Gas Liquid Chromatography equipped with Electron Capture Detector GC-ECD was used to detect the contamination in the samples. The results showed that p,p'-DDE isomer was dominated over the other isomers in all analyzed fish samples, followed by a-isomer of hexachlorocyclohexane. The concentrations of OC residues were higher than SP pesticides in all fish muscles. Also, the fresh fish muscle recorded higher concentrations of the evaluated pesticides than the grilled one.
Show more [+] Less [-]WHEAT YIELD AND ANTIOXIDANT ENZYMES RELATIONSHIP UNDER DIFFERENT SOIL WATER CONTENT Full text
2010
M Abdel-Kader | Nemat Noureldin | M f | Luka Bechini
WHEAT YIELD AND ANTIOXIDANT ENZYMES RELATIONSHIP UNDER DIFFERENT SOIL WATER CONTENT Full text
2010
M Abdel-Kader | Nemat Noureldin | M f | Luka Bechini
The objectives of this study were to examine the relationship between wheat yield cultivars (Sakha 93 and Giza 168) and antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and phenylalanine ammonia-lyase (PAL) activities under different soil water content i.e. low, moderate and high content by irrigation at 80% ETo (1280m³/fed.), by 100% ETo (1600m³/fed.) and120% ETo (1920m³/fed.), respectively. Two lysimeter experiments were carried out in two successive seasons i.e. 2006/2007 and 2007/2008 to estimate wheat yield and one experiment in plastic bags carried out of the greenhouse to determine the antioxidant enzymes of 28 days wheat plant under three different soil water content i.e. providing wheat seedlings with aforementioned treatment. Exposing wheat cultivars i.e. Giza 186 or Sakha 93 to water stress by irrigation at 80% ETo (1280 m³/fad.) exerted an increase in CAT, SOD, POD, APX and PAL over those irrigated at 100% ETo (1600 m³/fad.) or at 120% ETo (1920 m³/fad.), but the previous enzyme activities in the tissue of Saka 93 surpassed of that of Giza 168 cultivar. spike weight and weight of 100 grain/plant of Sakha 93 cultivar overcome that of Giza 168 at low soil moisture content (80% ETo), whereas opposite results were obtained at high and moderate water content (120% and 100% ETo) in the first growing season (2006/2007) and spike weight/plant in second growing season (2007/2008). There was a proportional relationship between increasing soil moisture content and grain yield/plant but the difference between moderate water content 100% ETo (1600 m³/fed.) and high water content 120% ETo (1920 m³/fed.) was not significant. Providing 28 days wheat plant with low water content, decreased wheat yield/plant and its attributes comparing with moderate water content for the two studied cultivars. The current study indicates that Sakha 93 cultivar was the most tolerant cultivar compared with Giza 168.
Show more [+] Less [-]Wheat yield and antioxidant enzymes relationship under different soil water content
2010
Abdel-Kader, M.A. | Nour El-Din, N.A. | Fawzy, M.H. | Bechini, L.
The objectives of this study were to examine the relationship between wheat yield cultivars (Sakha 93 and Giza 168) and antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and phenylalanine ammonia-lyase (PAL) activities under different soil water content i.e. low, moderate and high content by Irrination at 806/6 ETo (1280m'/fed.), by, 100% ETo (1600m'/fed.) and120% ETo (1920m'/fed.), respectively. Two lysimeter experiments were carried out in two successive seasons i.e. 2006/2007 and 2007/2008 to estimate wheat yield and one experiment in plastic bags carried out of the greenhouse to determine the antioxidant enzymes of 28 days wheat plant under three different soil water content i.e. providIng wheat seedlings with aforementioned treatment. Exposing wheat cuitivars i.e. Giza 186 or Sakha 93 to water stress by Irrigation at 80% ETo (1280 m3/fad.) exerted an increase in CAT, SOD, POD, APX and PAL over those irrigated at 100% ETo (1600 m'/fad.) or at 120% ETo (1920 m'/fad.), but the previous enzyme activities in the tissue of Saka.93 surpassed of that of Giza 168 cuitivar. spike weight and weight of 100 grain/plant of Sakha 93 cuitivar overcome that of Giza 168 at low soil moisture content (80% ETo), whereas opposite results were obtained at high and moderate water content (120% and 100% ETo) in the first growing season (2006/2007) and spike weight/plant in second growing season (2007/2008). There was a proportional relationship between increasing soil moisture content and grain yield/plant but the difference between moderate water content 100% ETo (1600 m'/fed.) and high water content 120% ETo (1920 m3/fed.) was not significant. Providing 28 days wheat plant with low water content, decreased wheat yield/plant and its attributes comparing with moderate water content for the two studied cuitivars. The current study indicates that Sakha 93 cultivar was the most tolerant cuitivar compared with Giza 168.
Show more [+] Less [-]Effect of harvesting dates on the yield and quality of some flax genotypes Full text
2010
El-Kady, E. | Salama, A.S. | Abd-El-Fattah, A.A.
Effect of harvesting dates on the yield and quality of some flax genotypes
2010
El-Kady, E. | Salama, A.S. | Abd-El-Fattah, A.A.
Two field experiments were conducted during the two successive seasons 2007/2008 and 2008/2009 at Sakha Agric. Res. Station, Kafr ElSheikh Governorate to study the effect of four harvesting dates i.e. 135, 145, 155 and 165 days after sowing on yield and its quality of six flax genotypes namely, Sakhal, Sakha 2, Sakha 3 and Giza 9 varieties and two new promising strains (strain 16 and strain 22). This study was laid out in a split-plot design, with four replications, the main plots were allocated to the six flax genotypes, whereas, the sub-plots contained the harvesting dates. The obtained results could be summarized as follow: Sakha 3 and Giza 9 varieties gave the highest means for technical length, fiber yield/plant as well as per fed., fiber length, fiber percentage, fiber strength, fiber fineness, germination % and crude protein %, whereas strain 22 (yellow seed) surpassed the other flax genotypes which produced the highest values for No. of seeds/plants, seed yield/fed., oil content and oil yield/fed. The harvest date of 155 days after sowing showed significant increases in stem diameter, straw yield/plant as well as per fed. fiber yield and its quality and crude protein % compared to the other three dates (135, 145 and 165 days after sowing). However, delayed harvesting date to 165 days after sowing recorded significant increase in technical length, upper branching zone length, seed yield and its related characters, germination %, seedling vigour, seed index, volume of 1000 seed,'oil content and oil yield/fed. The interactions among the studied factors had significant effects on straw yield/fed., No. of capsules/plants, fiber strength and germination % in the first season of study, while the characters of the upper branching zone length, seed yield/fed., oil yield/fed., fiber length and oil content in both seasons.
Show more [+] Less [-]EFFECT OF HARVESTING DATES ON THE YIELD AND QUALITY OF SOME FLAX GENOTYPES Full text
2010
Eman El-Kady | Samia Salama | A abd El-Fatah
ABSTRACT Two field experiments were conducted during the two successive seasons 2007/2008 and 2008/2009 at Sakha Agric. Res. Station, Kafr El-Sheikh Governorate to study the effect of four harvesting dates i.e. 135, 145, 155 and 165 days after sowing on yield and its quality of six flax genotypes namely, Sakha1, Sakha 2, Sakha 3 and Giza 9 varieties and two new promising strains (strain 16 and strain 22). This study was laid out in a split-plot design, with four replications, the main plots were allocated to the six flax genotypes, whereas, the sub-plots contained the harvesting dates. The obtained results could be summarized as follow: Sakha 3 and Giza 9 varieties gave the highest means for technical length, fiber yield/plant as well as per fed., fiber length, fiber percentage, fiber strength, fiber fineness, germination % and crude protein %, whereas strain 22 (yellow seed) surpassed the other flax genotypes which produced the highest values for No. of seeds/plants, seed yield/fed., oil content and oil yield/fed. The harvest date of 155 days after sowing showed significant increases in stem diameter, straw yield/plant as well as per fed. fiber yield and its quality and crude protein % compared to the other three dates (135, 145 and 165 days after sowing). However, delayed harvesting date to 165 days after sowing recorded significant increase in technical length, upper branching zone length, seed yield and its related characters, germination %, seedling vigour, seed index, volume of 1000-seed, oil content and oil yield/fed. The interactions among the studied factors had significant effects on straw yield/fed., No. of capsules/plants, fiber strength and germination % in the first season of study, while the characters of the upper branching zone length, seed yield/fed., oil yield/fed., fiber length and oil content in both seasons.
Show more [+] Less [-]Effect of treatment on the nutritive value and residues of some synthetic pesticides in fresh bolti fish
2010
Ahmed, N.S. | El-Saad, El.O.
Fresh Bolti -fish (Tilpia nilotica) collected randomly from 9 different markets in Ismailia Governorate for evaluation the effect of grilled fish by the method used in grillrooms and houses on the concentration of pesticide residues found and the nutritive value. Results revealed that decrease in the estimated parameters i.e. moisture, crude protein, fat and ash by grilling, this decrease were 6.07, 2.63, 4.07 and 1.56%, respectively. On the contrary, carbohydrates behaved another behavior that there was an obvious increase ranged from 1.98% to 2.92%. Fresh and grilled fish were analyzed to detect 12 organochlorine (OC) and 7 synthetic pyrethroid (SP) pesticides with a mean level on a lipid basis. Gas Liquid Chromatography equipped with Electron Capture Detector GC-ECD was used to detect the contamination in the samples. The results showed that p,p'-DDE isomer was dominated over the other isomers in all analyzed fish samples, followed by q-isomer of hexachlorocyclohexane. The concentrations of OC residues were higher than SP pesticides in all fish muscles. Also, the fresh fish muscle recorded higher concentrations of the evaluated pesticides than the grilled one.
Show more [+] Less [-]