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Characterization of Tritrichomonas foetus antigens, using bovine antiserum.
1986
Hall M.R. | Huang J.C. | Ota R. | Redelman D. | Hanks D. | Taylor R.E.L.
Modified indirect fluorescent antibody test for the serodiagnosis of Anaplasma marginale infections in cattle.
1985
Montenegro James S. | James M.A. | Ristic M.
Comparison of soluble antigens Leptospira interrogans serovars by SDS-PAGE, crossed immunoelectrophoresis and immunoblotting.
1992
Baik Y.O. | Mah J.S.
Avidin-biotin complex for immunohistochemical diagnosis of Aujeszky's disease and hog cholera.
1990
Kim S.B. | Sur J.H. | Moon U.G.
Immunohistochemical identification of Newcastle disease virus with indirect immunoperoxidase technique.
1990
Nho W.G. | Sur J.H. | Kim S.B.
Histopathological observations and virus detection by in situ hybridizatio in wild rats intranasally infected with Aujeszky's disease virus isolated in Korea
1999
Song, G.S. (Yuhan Research Center, Kunpo (Korea Republic).) | Moon, O.K. (Ministry of Agriculture Forestry, Anyang (Korea Republic). National Veterinary Research and Quarantine Service) | Jeong, C.G. | Kim, S.B. (Gyeongsang National University, Chinju (Korea Republic). Institute of Animal Medicien, College of Veterinary Medicine)
The present study was carried out to investigate the pathogenicity and pathogenesis of wild rats(Rattus norvegicus), trapped in nature, intranasally infected Aujeszky's disease virus(ADV/NYJ-1-87) by histopathology, immunohistochemistry and in situ hybridization(ISH). Fifteen rats inoculated intranasally were roughened haircoat, anorexia, listlessness, and depression second day after inoculation, and three rats died in 66-72 hours. Eight rats showed severe pruritus at the fact that was accompanied by frequent face-washing movements of the forelegs, and then became violent and spasmodic for and hour or until they died. Four rats slowly recovered after showing mild clinical signs of the disease. Microscopic lesions in infected rats were characterized by meningitis, perivascular round cell infiltration, focal gliosis, and neuronal degeneration and necrosis. And intranuclear inclusion bodies were frequently detected in the cerebral cortex and medulla. Positive reaction to ADV by immunohistochemistry and ISH were detected in the following areas:trigemimal ganglion, brain, tonsil, nasal mucosa, spleen, lung and liver. The result has suggested that ADV intranasally infected in wild rats is followed by replication in epithelial cells of nasal mucosa and tonsil, then invade local lymph nodes by way of the lymphatics. It is also believed that the virus invades bipolar olfactory cells and trigerminal ganglion, and then spread into central nervous system.
Show more [+] Less [-]Production and partial purification of Staphylococcus aureus alpha toxin
1999
Park, H.M. | Oh, T.H. | Han, H.R. (Seoul National University, Suwon (Korea Republic). Department of Veterinary Internal Medicine, College of Veterinary Medicine)
Alpha toxin of S aureus has cytolytic activity respectively. This antigen has been received the most attention since it is a major virulence factor in pathogenesis of staphylococcal mastitis. Thus, alpha toxin has been focused as potential candidate ofvaccine tominimize mastitis in cows. the purpose of this study was to develop a simple, efficient production and purification methods of sufficient amount of alpha toxin antigen from S aureus. Alpha toxin production measured by hemolytic activity was the highest at 18 hrs postinoculation in yeast extract culture medium supplemented with thiamine, nicotinic acid and casamino acid. Alpha toxin was purifed by ammonium sulfate precipitation (65%) and ultrafiltration. Molecular weight of the toxin was 33 kDa in the analysis with SDS-PAGE. Conclusionally, when alpha toxin was included in the vaccine, the optimal harvest time of alpha toxin was at 18 hrs after inoculation in yeast extract medium supplemented with thiamine and nicotinic acid.
Show more [+] Less [-]Sequence analysis of the variable VP2 gene of infectious bursal disease viruses isolated in Korea
1999
Kwon, H.M. | Kim, D.K. (Kangwon National University, Chuncheon (Korea Republic). Department of Veterinary Medicine) | Seong, H.W. (National Veterinary Research and Quarantine Service, Anyang (Korea Republic).)
A 474-base pair segment covering the hypervaible region of VP2 gene from six Korean infectious bursal disease virus(K-IBDV) isolates(K1, K2, SH/92, 225, 269, 310) and one attenuated IBDV(DAE) were amplified using RT-PCR, sequenced, and compared with published sequences for IBDV. K-IBDV isolates(K1, K2, SH/92, 225, 269) and foreigh very virulent(vv) IBDV strains had 94.93-100% amino cid sequence similarity. K-IBDV isolate 310 and other K-IBDV isolates had 84.31%-86.07% amino acid sequence similarity. Attenuated strain(DAE), like other attenuated strain, has substitution at positions 279(D to N) and 284(A to T) as well as in the serine-rich heptapeptide region. Five K-IBDV isolates except 310 isolate share unique amino acid residues at positions 222(A), 256(I), 294(I) which are not present in other standard and attenuated strains. At the two hydrophilic region, K-IBDV isolates except 310 isolate had identical amino cids comparing with Belgium vv IVDV 894VB but had four amino acid substitutions comparing with Chinese vv IBDV F9502. The SWSASGS heptapeptide is conserved in all KIBDV isolates. The sequence of K-IBDV isolate 310 was markedly different from other IBDV strains, evolving from a separate lineage than the others. By phylogenetic analysis, Five K-IBDV isolates except 310 isolate were categorized in one group with foreign vv IBDV isolates but K-IBDV isolate 310 was categorized ina separate group which was differentiated form other compared IBDV strains
Show more [+] Less [-]Drug susceptibility of bacteria and M pachydermatis isolated from canine external ear canals
1999
Kim, K.H. | Choi, W.P. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Yeo, S.G. (Gyeongsang National University, Chinju (Korea Republic). College of Vetrinary Medicine)
The present work was conducted to investigate the drug susceptibility of microorganisms isolated form canine external ear canals. Antifungal susceptibility test of M pachydermatis(17 strains) was performed by agar dilution method, using 11 antifungal drugs including amphotericin B(A), nystatin(N), pimaricin(P), griseofulvin(G), gifonazole(B), clotrimazole(C), miconazole(M), econazole(E), ketoconazole(K), tolnaftate(T), 5-fluorocytosine(F). All isolates were highly sensitive to K, M, T(geometric mean MIC;GM MIC_0.16 micro gram/ml). Antibacterial susceptibility test against 119 isolates of bacteria was performed by agar dilution method, using 9 antibacterial drugs including erythromycin(ET), chloramphenicol(CP), gentamycin(G), vancomycin(V), ampicillin(AP), amoxacillin(AX), chlortetracycline(CT), ciprofloxacin(CF), enrofloxacine(EF). All isolates of Staphylococcus spp(101 strains) were highly sensitive to EF, CF, G(GM MIC 0.33~1.47 micro gram/ml). In other gram positive cocci(4 strains), they were highly sensitive to EF, CF, V(GM MIC 1~4.76 micro gram/ml) and CT(GM MIC 1 UFL unit/ml). In gram positive rods(13 strains), they were highly sensitive to EF, CF, G(GM MIC_0.19~1 micro gram/ml). In Pseudomonas aeruginosa(1 strain), it was highly sensitive to AX, EF, ET, CF(GM MIC 0.06~1 micro gram/ml) and CT(GM MIC 1 UFL unit/ml). All isolates weren't sensitive to AP(GM MIC 16~32 micro gram/ml).
Show more [+] Less [-]Proteome analysis: Salmoenlla enteritidis antigen proteins
Park, M.R.;Shin, Y.S.;Han, D.Y.;Kim, Y.H.;Jung, T.S.;Lee, H.J.;Kim, J.S.;Kim, G.S.(Gyeongsang National University, Jinju, Republic of Korea)E-mail:gonskim@nongae.gsnu.ac.kr | Lee, E.G.(Sungkyunkwan University, Suwon, Republic of Korea) | Kim, E.H.(Jinju International University, Jinju, Republic of Korea)
The common pathogen Salmonella enteirtidis (S. enteritidis) is the major cause of foodborne disease. Protein identification by peptide mass fingerprinting using the matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analysis unambiguously identity the spots from 2-dimensional electrophoresis (2-DE) gel. In this report, we examined protein components from patterns of S. enteritidis proteins. In addition, antigens that are recognized by sera can be identified by immunoblotting.
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