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Proteome analysis: Salmoenlla enteritidis antigen proteins
Park, M.R.;Shin, Y.S.;Han, D.Y.;Kim, Y.H.;Jung, T.S.;Lee, H.J.;Kim, J.S.;Kim, G.S.(Gyeongsang National University, Jinju, Republic of Korea)E-mail:gonskim@nongae.gsnu.ac.kr | Lee, E.G.(Sungkyunkwan University, Suwon, Republic of Korea) | Kim, E.H.(Jinju International University, Jinju, Republic of Korea)
The common pathogen Salmonella enteirtidis (S. enteritidis) is the major cause of foodborne disease. Protein identification by peptide mass fingerprinting using the matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analysis unambiguously identity the spots from 2-dimensional electrophoresis (2-DE) gel. In this report, we examined protein components from patterns of S. enteritidis proteins. In addition, antigens that are recognized by sera can be identified by immunoblotting.
Show more [+] Less [-]Isolation and characterization of Brachyspira pilosicoli in Korean dogs
Kim, T.J.;Lee, J.I.(Chonnam National University, Gwangju, Republic of Korea)E-mail:jaeil@chonnam.ac.kr
This study was performed to report the outbreaks of canine intestinal spirochetosis and to characterize the canine isolates. Three canine isolates were weakly beta-hemolytic and had sharp end shape with 5 flagella. Isolates didn't produce indole but fermented fructose. In API-ZYM∨_ study, isolates were alpha-glucosidase negative and alpha-galactosidase positive, which is the typical characteristics of B. pilosicoli. In multilocus enzyme electrophoresis(MEE) study, isolates were divided into 2 electrophoretic types.
Show more [+] Less [-]Analysis of gamma-ray-induced DNA damage in human, mouse and rat peripheral blood lymphocytes using single-cell gel electrophoresis
Oh, H.;Jung, U.H.;Park, H.R.;Jo, S.K.(Korea Atomic Energy Research Institute, Daejeon, Republic of Korea)E-mail:skjo@kaeri.re.kr | Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)
The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of ∨60Co γ-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p less than 0.01) and TL (p less than 0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs.
Show more [+] Less [-]Virulence-associated plasmids of Salmonella spp, isolated from animals in Korea
1992
Choi, W.P. | Jung, S.C. (Kyungpook National University, Taegu (Korea Republic). Department of Veterinary Medicine)
Studies on hemoglobin type in Cheju native horse
1991
Hyun, H.S. | Kim, W.K. | Han, B.K. (Chonnam National Univ., Kwangju (Korea Republic). Coll. of Veterinary Medicine)
Studies on the electrophoretic analysis of serum proteins in dogs infected with Babesia gibsoni
1991
Ihn, D.C. | Lee, J.M. | Chae, J.S. | Cha, H.S. (Chonbuk National Univ., Chonju (Korea Republic). Coll. of Veterinary Medicine)
Biological characteristics of Escherichia coli isolated from pigeons
1990
Seo, D.K. | Choi, W.P. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Park, N.C. (Kyungpook Animal Health Laboratory, Taegu (Korea Republic))
Monoclonal antibodies against structural proteins of bovine viral diarrhea virus
1992
Kweon, C.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Inst.) | Chun, Z.Y. (Univ. of California Davis, California (USA). Dept. of Veterinary Microbiology and Immunology) | Woo, H.J. (Harvard Medical School, Boston (USA). Lab. of Cancer Biology)
Electrophoretic analysis of the major proteins of race horse erythrocyte membrane: Their relation to fast erythrocyte sedimentation rate.
1991
Bahk, Y.W. (Kwangju Health Junior Coll., Kwangju (Korea Republic). Dept. of Clinical Pathology) | Lee, S.Y. (Korea Horse Affairs Association, Kwachon (Korea Republic). Equine Health Research)
