Jeotgal (salted seafood) has been one of major fermented foods in Korea for long time. Although there are many studies about Jeotgal in various aspects of food, its immunological importance on hosts has not been elucidated yet. In this study, we investigated if several bacteria isolated from Jeotgal may modulate the function of dendritic cells (DCs), powerful antigen-presenting cells equipped with special immunological capabilities. 4 Jeotgal bacteria were selected as representatives and used for experiments. To treat viable DCs, those bacteria were killed at 60¡� for 30 min. The viability of DCs treated with Jeotgal bacteria was verified and two isolates significantly induced high production of interleukin-12, a representative cell-mediated cytokine of DCs. Surface activation and maturation markers (MHC class II, CD40, CD86) of DCs were analyzed by flow cytometer. In addition, the treated DCs showed significantly high lymphocyte stimulatory capability compared to control DCs based on allogeneic mixed lymphocyte reactions. These observations suggest that Jeotgal isolates can function as immunostimulating bacteria in hosts, like Lactobacillus. Taken together, these experimental evidences may broaden the use of Jeotgal isolates in immunological fields in addition to as a fermented food.

Survivability of Listeria monocytogenes in clay loam soil was examined at 30° C for a period of 35 days. The microorganism inoculated in soil steadily declined from 3.5 × 10 6 g -1 to 3.9 × 10 4 g -1 (P = 0.05) on 35 th days. Destruction of normal soil microflora by heat treatment resulted in marginal rise in 3.3 × 10 6 g -1 of L. monocytogenes to 4.2 × 10 6 g -1 during the first 7 days, followed by steady decline to 5.6 × 10 4 g -1 on 35 th days. Heat treated soil inoculated with lower concentration of L. monocytogenes (3.6 × 10 4 g -1 ) supported sharp rise in its population to 4.8 × 10 6 g -1 (P=0.05) during the first weak which gradually declined, but remained higher (6.1 × 10 4 g -1 ) than the initial load even on 35 th days. In non-sterile soil, the rise was less appreciable but L. monocytogenes maintained its levels (10 4 g -1 ) during the experimental period. Survivability of the microorganisms was also observed in the soil with added chicken manure, cattle manure or sheep/goat manure.

Investigation of antimicrobial activity of liquid extractions from the following medicinal plants and their mixtures against S. aureus, B. subtilis, E. coli, P. aeruginosa, and P. vulgaris was realized in the conditions of the Republic of Belarus: peat moss (Sphagnum); greater plantain (Plantago major); plumepoppy (Macleaya); common comfrey (Symphytum officinale); speckled alder (Alnus incana). Research results showed that the best results for septic wounds treatment were obtained apter application of mixture of plumepoppy and greater plantain as it showed the acute antimicrobial, anti-inflammatory, and wound-healthing effect. Antimicrobial activity of the analyzed infusion formula revealed in quicker (on 3-6 days in comparison with control) wound cleansing from microorganisms, quick lowering of bacterial content up to 10E3 bodies per 1 g of tissue and in provision of wound healing without inclusion of saprogenous microflora. Anti-inflammatory effect was characterized by quick (by 2 days) cancellation of acute inflammatory response, that was revealed in lowering of neutrophilic leukocytes quantity up to 59,8 +/- 0,49 %; quicker normalization hematologic indexes and early reduction of perifocal edema; weakening of hyperthemia and hyperemia; decreasing of wound secretion without purulence; absence of leaks and leak pouch; normalization of microcirculation in wound. Wound-healthing effect was revealed in increasing of fibroblast number from 7,1 +/- 0,4 % up to 29,4 +/- 0,4 % on the 7th day and in activation proliferate and synthetically function

Determination of influence of long acting antihelminthics (Aversictini boluses and boluses with albendazole) and influence of usual antihelminthics (farmatsin, alverm, fenbendavet) on first stomachanimal digestion, as well as on quantity and quality composition of microflora and microfauna in first stomach and large bowel was realized in the conditions of the Republic of Belarus. In course of the experiment there were used 6 groups of calves infested with helminths (Neoascaris vitulorum, Strongylata sp.). Research result showed that different antihelminth preparations to a greater or lesser degree rendered negative influenced on quantity and quality composition of microorganisms in first stomach and large bowel of cattle. Alverm and boluses with albendazole rendered the least influence on microflora and microfauna that could be explained by low toxicity of active substance (albendazole) of the above preparations. Aversictini boluses, fenbendavet, and farmastin changed the composition of microorganisms. It could be explained by high toxicity of preparations containing aversictini which rendered influence not only of gastro-intestinal tract but also on the whole organism. Active ingredient of mentioned above preparations (Aversektin C), being the product of microbial synthesis, rendered the antagonist action on the normal microflora of digestive tract. Negative influence of fenbendavet was caused by the chemical composition of the preparation and neurotoxic action of fenbendazol

Results of study of a pollution level of drinking water around a pig-breeding complex and degree of its distribution in Gorodok district of the Republic of Belarus were presented. For carrying out the monitoring of water objects around the pig-breeding complex there was analysed the drinking water in Palminka and Gorodok localities. Water tests were taken once in three months (September, December, March, June), in the morning. Tests of drinking water used on complexes, were taken once a month. Research results showed that drinking water quality on the pig-breeding complex was considerably lower in comparison with the remoted from it water sources. Closeness of the pig-breeding complex lowered the water quality both on the territory of swine complex and on the territory of neighboring objects. Results of the chemical water analysis, organoleptic estimation, and water correspondence to the sanitary-hygienic standard were presented