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Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat Full text
2018
Różycki, Mirosław | Chmurzyńska, Ewa | Bilska-Zając, Ewa | Karamon, Jacek | Cencek, Tomasz
Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat Full text
2018
Różycki, Mirosław | Chmurzyńska, Ewa | Bilska-Zając, Ewa | Karamon, Jacek | Cencek, Tomasz
Health, religious, and commercial aspects justify the need for meat species identification. The lack of officially approved methods prompts the undertaking of research on validation of isoelectric focusing of proteins (IEF) for official purposes. Samples were prepared from pigs (Sus scrofa ferus domestica), cattle (Bos taurus), and poultry (Gallus gallus domesticus). Meat mixtures were made by blending 50%, 25%, 10%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.2% meat of other species. Samples were examined on ultrathin polyacrylamide gels with pH 3–9 gradient. The results of the study confirmed the stable and reproducible pattern of meat protein bands. The detection limit of raw meat admixtures from pigs, cattle, and poultry mostly ranged from 2% down to 0.2% (0.2% for poultry). However, the IEF method can be used to detect the addition of pig meat to bovine meat in an amount higher than 3%. At the significant mixture level (i.e at least 5% addition of meat of another species) IEF proves itself with 100% specificity, sensitivity, and accuracy. The achieved detection limits provide a basis for recommending the IEF method for routine tests in laboratories detecting the species origin of meat.
Show more [+] Less [-]Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat Full text
2018
Różycki Mirosław | Chmurzyńska Ewa | Bilska-Zając Ewa | Karamon Jacek | Cencek Tomasz
Health, religious, and commercial aspects justify the need for meat species identification. The lack of officially approved methods prompts the undertaking of research on validation of isoelectric focusing of proteins (IEF) for official purposes.
Show more [+] Less [-]Usefulness of an anesthetic mixture of medetomidine, midazolam, and butorphanol in cotton rats (Sigmodon hispidus) Full text
2016
Nakamura, T. ((Japan Food Research Laboratories, Chitose, Hokkaido (Japan). Chitose Laboratory, Section of Biological Safety Research), (Hokkaido University, Sapporo, Hokkaido (Japan). Graduate School of Veterinary Medicine, Department of Biomedical Sciences, Laboratory of Anatomy)) | Ichii, O. | Irie, T. | Hosotani, M. | Dantsuka, A. | Nakamura, S. | Sato, S. | Sotozaki, K. | Kouguchi, H. | Yoshiyasu, T. | Nagasaki, K. | Kon, Y.
Milk Constituents and Microbial Analysis of Mixed Milk Samples in Rural Areas.
2009
Sreedhar, S. | Suresh Babu, D.
The study found out the chemical and microbial quality of various milk samples collected from different sources of milk procured from rural areas of Tirupati. Thefat, SNF and protein percentages of milk samples collected from Dairy Experimental Station were higher than the other milk samples collected from commercial dairies of Sangam, Balaji and Heritage and local vendors. The milk samples collected from local vendors showed lower fat, SNF and protein percentages. No significant difference was observed in the milk samples collected from commercial dairies of Sangam, Balaji and Heritage with regard to fat, SNF and protein percentages. None of the milk samples collected from Dairy Experimental Station were found to be adulterated with water, whereas higher percentage of water was observed in the milk samples of local vendors. The Standard Plate Count of milk from local vendors is higher followed by Dairy Experimental Station. Presence of E.coli organisms in the milk samples collected from local vendors and Dairy Experimental Station indicated the extraneous contamination and unhygienic conditions of milking barn.
Show more [+] Less [-]Anesthetic effect of a mixture of medetomidine, midazolam and butorphanol in chickens with antagonism by atipamezole Full text
2019
Inoue, A. (Fukuyama University, Fukuyama, Hiroshima (Japan). Faculty of Pharmacy and Pharmaceutical Sciences, Department of Pharmacotherapeutics)) | Ochi, T.
