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Antigenetic effects of the eluted proteins from house dust mite (Dermatophagoides pteronyssinus) in dogs infested with sarcoptic mite (Sarcoptes scabiei var. canis)
2005
Kim, T.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, J.W. (Chungbuk National University, Cheongju, Republic of Korea) | Jee, C.H. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
Canine sarcoptic mite (Sarcoptes scabiei var. canis) is ectoparasite which burrow usually in the stratum corneum of the skin of dogs. Antigens from the burrowing mites induce humoral and cellmediated immune responses in the hosts. The effect of antigenecity induced by somatic antigens of house dust mite (Dermatophagoides pteronyssinus) isolated by continuous elution has been evaluated in canine sarcoptic mites infestation. Continuous elution was carried out in 7.5% SDS-PAGE to isolate proteins of common antigens from somatic antigens of house dust mite.
Show more [+] Less [-]Antibiotic resistance pattern of Enterococcus spp. and Staphylococcus aureus isolated from chicken feces
2005
Lee, Y.J. (Kyungpook National University, Daegu, Republic of Korea), E-mail: [email protected] | Kim, A.R. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jung, S.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Song, S.W. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, J.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
This study was carried out to investigate the antibiotic resistance pattern of Enterococcus spp. and Staphylococcus aureus (S. aureus) isolated from chicken feces. All isolates showed high resistance to erythromycin (E) and tetracycline (TE). Of the 63 Enterococcus faecalis (E. faecalis) isolates, 73.0% were resistant to E and 98.4% to TE. Of the 44 Enterococcus faecium (E. faecium) isolates, 50.0% were resistant to E and 95.5% to TE. Of the 52 S. aureus isolates, 57.6% were resistant to E and 96.2% to TE. The prevalence of two and three drugs resistance pattern were 28.6% and 17.5% of E. faecalis, 40.9% and 25.0% of E. faecium and 38.5% and 23.1% of S. aureus, respectively.
Show more [+] Less [-]Nucleotide sequence analysis and expression of NSP4 gene of avian rotavirus
2005
Shin, I.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, W.Y. (Chungang University, Seoul, Republic of Korea) | Lee, S.C. (Chungbuk National University, Cheongju, Republic of Korea) | Kang, S.Y. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene of avian rotavirus (AvRV-1, AvRV-2) was analyzed and expressed using baculovirus expression system. The sequence data indicated that the NSP4 gene of AvRV-1 and AvRV-2 were 727 bases in length, encoded one open reading frame of 169 amino acids beginning at base 41 and terminating at base 550, and had two glycosylation sites. Nucleotide sequences of NSP4 gene of AvRV-1 and AvRV-2 exhibited a high degree of homology (88.1±7.6%) with avian rotaviruses, namely Ty1, Ty3 and PO-13.
Show more [+] Less [-]Peripheral lymphocyte counts in Holstein-Friesian cattle infected with bovine leukemia virus in Korea
2005
Suh, G.H. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea), E-mail: [email protected] | Lee, C.G. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.C. (Seojeong College, Yangju, Republic of Korea) | Hur, T.Y. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Kang, S.J. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Son, D.S. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Ahn, B.S. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Kim, N.C. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Lee, C.Y. (Chonnam National University, Gwangju, Republic of Korea)
Hematologic investigations were made on the blood samples taken from bovine leukemia virus (BLV)-seropositive Holstein-Friesian cattle in Korea, and their absolute lymphocyte count was compared with that of BLV-seronegative cattle. The incidence of persistent lymphocytosis (PL) was also determined. The normal bovine lymphocyte count was established on the basis of studies of 656 blood samples taken three times from 297 seronegative animals aged from 0~6 months to over 5 years at 5~6-month intervals. The data were examined according to 7 age groups of samples placed into their respective age groups.
Show more [+] Less [-]Effects of space allowance on the social behavior of Korean native cattle (Bos taurus coreanae) steers
2005
Han, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Jeon, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, D.J. (Gyeongsang National University, Jinju, Republic of Korea) | Chang, H.H. (Gyeongsang National University, Jinju, Republic of Korea) | Koo, J.M. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, Y.K. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, S. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, E.J. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.C. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.J. (Gyeongsang National University, Jinju, Republic of Korea) | Yeon, S.C. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
This study was carried out to find out how space allowance affect the social behavior of Korean native cattle (Bos taurus coreanae) steers. Twelve Korean native cattle (Bos taurus coreanae) steers were used as subjects, each of which was 30-month-old and observation period was from June to July 2003. Five (T1) and seven (T2) steers were allotted to two pens of 5 m×10 m in a building with slate roof and open sides respectively. They were fed at 09:00 h and 16:00 h, twice a day. The behaviors of steers were recorded from 06:00 h to 17:00 h, using two color CCD cameras (Samsung SDC-411, Korea), one B/W CCD cameras (Samsung SBC-340, Korea), one multiplexer (Samsung SDM-081, Korea) and a time lapse VCR (Samsung SRV-30, Korea).
Show more [+] Less [-]A case of chronic lymphocytic leukemia (CLL) in a Maltese dog
2005
Lee, J.Y. (Konkuk University, Seoul, Republic of Korea) | Hong, E.S. (Konkuk University, Seoul, Republic of Korea) | Kang, B.T. (Konkuk University, Seoul, Republic of Korea) | Jung, D.I. (Konkuk University, Seoul, Republic of Korea) | Park, C. (Konkuk University, Seoul, Republic of Korea) | Park, H.M. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
An 11-year-old, 3.3 kg, male Maltese dog was referred to Veterinary Teaching Hospital of Konkuk University because of diarrhea and severe anemia. Abnormal physical examination findings included left submandibular lymph node enlargement, pale mucous membrane, cataract, and bloody diarrhea. Results of hematologic examination revealed a marked lymphocytosis resulting in leukocytosis and the markedly increased numbers of small, well-differentiated lymphocytes in the peripheral blood. Serum biochemical abnormalities consisted of elevated AST and ALP, hyperphosphatemia, hypoglycemia, and hypoalbuminemia.
Show more [+] Less [-]Hepatotoxicity in treatment of canine dermatophytosis with ketoconazole
2005
Bae, S.S. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, C.H. (Gyeongsangnam-do Livestock Promotion Institute, Jinju, Republic of Korea) | Kim, T.Y. (Ministry of Agriculture and Forestry, Kwacheon, Republic of Korea) | Kang, C.B. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
The purpose of this study is to compare hepatotoxicity of each treatment for dermatophytosis; one is the administration of the ketoconazole only and the other, ketoconazole with diphenyl-dimeththyl-dicarboxylate. Have chosen the range of 14-24 months of healthy dogs divided by two groups (group 1 and group 2) for the experiment of which test proved positive in dermatophytosis diagnosis and showed normal reaction in terms of physical examination, blood chemistry and especially of liver function. Group 1 was administrated ketoconazole orally at 10 mg/kg/day and of same dose of ketoconazole with diphenyl-dimethyl-dicarboxylate for group 2.
Show more [+] Less [-]Effects of bone healing capacity by autologous fibrin glue in experimental bone defect dogs
2005
Lee, J.I. (Chonbuk National University, Jeonju, Republic of Korea) | Song, H.N. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, N.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected]
This study was investigated of the bone healing capacity by autologous fibrin glue in experimental bone defect dogs. The autologous fibrin glue manufactured just before the experiment was mixed with the concentrated fibrinogen from whole blood of the experimental dog and bovine thrombin. The experimental group was constituted with seven dogs. The experimental osteotomy was performed 5 mm length in bilateral region of proximal diaphyseal fibulae. The defected regions of experimental group were filled with the autologous fibrin glue by duploject. The experimental regions had been radiographed biweekly for 16 weeks to observe new bone formation and union.
Show more [+] Less [-]Cadiorespiratory effects of isoflurane-anesthetized dogs with closed chest during spontaneous two-lung and one-lung ventilation
2005
Song, Y.S. (Konkuk University, Seoul, Republic of Korea) | Chang, H.S. (Konkuk University, Seoul, Republic of Korea) | Kim, H.J. (Konkuk University, Seoul, Republic of Korea) | Kim, S.S. (Konkuk University, Seoul, Republic of Korea) | Choi, J.C. (Konkuk University, Seoul, Republic of Korea) | Yang, H.T. (Konkuk University, Seoul, Republic of Korea) | Chung, D.J. (Konkuk University, Seoul, Republic of Korea) | Kim, H.Y. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
One-lung ventilation (OLV) is the isolation and selective ventilation of one lung field. OLV allows the collapse of lung lobes on the side of the thoracic surgical approach to facilitate observation of intrathoracic structures and to achieve lung immobility. OLV be achieved by endotracheal intubation with double lumen tubes or bronchial blockers. In this study, cardiopulmonary consequences of two-lung ventilation (TLV), OLV and Re-TLV (TLV after OLV) were evaluated in 5 dogs. The dogs were anesthetized with mask induction and maintained with isoflurane in oxygen. Tidal volume and respiratory rates were set to maintain end-tidal CO₂ at 40±2 mmHg during instrumentation.
Show more [+] Less [-]Examinations on sedation, anaesthetic and toxic effect of Isoeugenol for olive-flounder (Paralichthys olivaceus)
2005
Shin, G.W. (Gyeongsang National University, Jinju, Republic of Korea) | Oh, M.J. (Yosu Naitonal University, Yosu, Republic of Korea) | Joh, S.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Shin, Y.S. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, Y.R. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, E.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Yang, H.H. (Gyeongsang National University, Jinju, Republic of Korea) | Palaksha, K.J. (Gyeongsang National University, Jinju, Republic of Korea) | Huh, N.E. (Gyeongsang National University, Jinju, Republic of Korea) | Yeon, S.C. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.C. (Gyeongsang National University, Jinju, Republic of Korea) | Jung, T.S. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected] | Kang, S.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Olive flounder (Paralichthys olivaceus) is the major mariculture fish in Korea. The annual aquaculture production of olive flounder in Korea during the period of 2003 was 300,000 ton (2002 Statistics, Ministry of Maritime Affairs and Fisheries, Korea Government). Anesthetics is very necessary in aquaculture to minimize stress and damage during harvesting, grading, transportation, spawning induction and handling to fish. In the present study, isoeugenol as new anesthetic in marine fish, especially olive flounder, was examined to know the efficacy and proper concentration. As a result, olive flounder adult was exhibited sedation at 5 ppm at 10 and 15℃, and 7.5 ppm at 20℃, respectively.
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