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Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine
2018
Weiner Marcin | Żukiewicz-Sobczak Wioletta | Tokarska-Rodak Małgorzata | Plewik Dorota | Pańczuk Anna | Siłuch Marta | Zagórski Jerzy | Sobczak Paweł | Chmielewski Tomasz | Tylewska-Wierzbanowska Stanisława | Shkilna Mariia | Korda Mykhailo | Klisch Ivan | Andreychyn Mykhailo | Pavliuk Mariana
Lyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks.
Show more [+] Less [-]Salmonid alphavirus (SAV)
2018
Deperasińska Izabela | Schulz Patrycja | Siwicki Andrzej K.
Salmonid alphavirus (SAV), genus Alphavirus, family Togaviridae, is a single-stranded RNA virus affecting Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). It is known to be responsible for pancreas disease (PD) and sleeping disease (SD) which are increasing problems, causing high fish mortality and economic losses in the European aquaculture industry. Pancreas disease was first described in Atlantic salmon in Scotland in 1976 and a similar disease caused by the closely related sleeping disease virus was first described in rainbow trout in France. There have also been reports of salmonid alphavirus infections from other European countries, including Ireland, England, Norway, Germany, Italy, and Spain. Salmonid alphaviruses have been classified into six subtypes (SAV1–6). SAV1 and SAV4–6 cause pancreas disease in Atlantic salmon in Ireland or Scotland, SAV2 is the causative agent of sleeping disease in rainbow trout, and SAV3 has been detected in Atlantic salmon in Norway. The aim of this paper was to summarise current knowledge of infections caused by salmonid alphavirus and diagnostic methods including the newest techniques, and to briefly describe prevention from SAV infections by vaccination.
Show more [+] Less [-]Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow
2018
Snarska Anna | Wysocka Dominika | Rytel Liliana | Makowska Krystyna | Gonkowski Sławomir
Introduction: Bisphenol A (BPA) is a substance widely used in industry for the production of polycarbonates and epoxy resins used in packaging and containers for beverages, contact lenses, compact discs (CDs), window panes, and many other elements. This compound belongs to the group of polyphenols and xenoestrogens commonly found in the human environment. What we know about BPA is still insufficient to enable us to protect our health against its adverse effects, and current knowledge of the influence of BPA on erythroblastic cell lines in bone marrow is rather fragmentary. The aim of the experiment was to assess the effect of two doses of BPA (0.05 mg/kg and 0.5 mg/kg b.w. per day) on myeloid haematopoiesis.
Show more [+] Less [-]Amitraz marker residues in honey from honeybee colonies treated with Apiwarol
2018
Pohorecka, Krystyna | Kiljanek, Tomasz | Antczak, Maja | Skubida, Piotr | Semkiw, Piotr | Posyniak, Andrzej
Amitraz is a formamide exhibiting both acaricidal and insecticidal activity and is frequently used by beekeepers to protect honeybee colonies against Varroa destructor mites. The aim of this apiary trial was to evaluate the impact of honeybee colony fumigation with amitraz on the level of contamination of honey stored in combs. Experimental colonies were fumigated four times every four days with one tablet of Apiwarol per treatment. Honey was sampled from combs of brood chambers and combs of supers one day after each amitraz application and from harvested honey. Amitraz marker residues (as a total of amitraz and metabolites containing parts of molecules with properties specific to the 2,4-DMA group, expressed as amitraz) were evaluated in honey. All analysed samples were contaminated with amitraz metabolites. 2,4-DMA and DMPF were the most frequently determined compounds. The average concentration of amitraz marker residue in honey from groups where a smouldering tablet was located directly in beehives was significantly higher than that of residue in honey from groups with indirect smoke generation. No significant effect on the honey contamination deriving from the place where it was exposed to smoke (combs of brood chambers and supers) was noted. Amitraz marker residues exceeded the MRL in 10% of honey samples from combs. Fumigation of beehives with amitraz results in contamination of honey stored in combs.
Show more [+] Less [-]Histamine content in rennet ripening cheeses during storage at different temperatures and times
2018
Madejska, Anna | Michalski, Mirosław | Pawul-Gruba, Marzena | Osek, Jacek
In recent years, there has been a great interest in biogenic amines such histamine, as they are associated with the quality and safety of some kinds of fermented foods. The aim of this study was to evaluate the effect of temperature and storage time on the content of histamine in cheeses. Samples of mould and hard cheeses were examined with RP-HPLC with an organic-aqueous mobile phase containing acidic buffer and chaotropic salt. The samples were stored either at 22 ± 2°C for 42 days (mould and hard cheeses) or at 4 ± 2°C for 112 days (mould cheeses) and 133 days (hard cheeses). The mean total histamine content in cheeses stored at 22°C was higher than the content in those stored at 4°C, with the highest concentrations found in Gorgonzola Piccante cheese (730.47 mg/kg). Histamine concentration in some types of cheeses exceeded the toxic threshold dose, indicating that after long or inadequately cool storage they may not be safe for consumers. To protect cheeses from contamination with histamine-producing bacteria and to safeguard consumers from poisoning, factors conducive to this amine’s formation should be minimised during cheese processing. Suitable temperature and time during storage of cheeses are recommended to avoid the intoxication. Monitoring of this toxin in food is necessary to ensure safety of consumers.
Show more [+] Less [-]In-Situ Transitional Cell Carcinoma of Urinary Bladder in a Cat
2018
ÖZTÜRK, Hazal | Egeden, Evrim | Egeden, Özlem | Gürel, Aydın
Urinary bladder tumors in cats, unlike in dogs, usually appear outside of the trigonal region as localization. These tumors are confused withurinary tract infections associated with hematuria in a clinical sense. Cytological examination and ultrasound (USG) imaging techniques arevery valuable, but histopathological approach is the golden key. In this case neutered male, mixed, 17-year-old cat was brought to the clinicwith non-obstructive hematuria attacks. A superficial hypoechoic mass located at the apex of the urinary bladder was detected in the USG imagingtechnique. No discernible findings were found by cytological examination. After removal of the suspected area, the biopsy specimen waspresented to the pathology department for histopathological examination. Histopathological examination revealed transitional cells coveringentire surface of the mucosal epithelium and showing growth into the lumen, and they were characterized by mild anisocytosis and anisokaryosis.The patient was diagnosed as in-situ non-papillary, non-infiltrating type of transitional cell carcinoma (in-situ carcioma). These tumorsare quite rare and have better prognosis. The condition of the patient was completely resolved without medical treatment in the postoperativeperiod. When this case report was prepared, 6 months after the operation, there was no recurrence in the patient. Contrary to infiltrative urinarybladder tumors, in-situ carcinoma could be completely cured by surgically, therefore the case was found worthy to be presented.
Show more [+] Less [-]A Case of Meningocele in an Akkaraman Lamb
2018
Tanrisever, Murat | Unsaldi, Emine
A meningocele case has been observed on a one-day-old male Akkaraman lamb, brought to the Surgery Clinic, Animal Hospital and University of Fırat. When we examined the animal, there was a fluctuating mass about 15 cm diameter size in the occipital area. We decided to surgical operating to this case of meningocele which is rare and usually does not respond favorably to treatment. Under a general anesthesia firstly liquid was drained out from the mass by using cannula. Then the excess skin piece was removed with the incision in a controlled manner. Approximately 2 mm diameter hole was closed which opened into the medullary canal by using sutures. At the same time, processus spinosus of first cervical vertebrae was seen that the 2 cm longer than normal. After cutting the excess bone part, the head skin was sutured with using 2-0 suture. The patient received parenteral antibiotics for 5 days. After 2 months the operation, it was observed that he continued his life in a very healthy. This case was thought to report because it is a rarely seen type of anomaly in lamb and respond favorably to the operation in this case.
Show more [+] Less [-]The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season
2018
Ata, Ayhan | Enes Inanc, Muhammed | Gungor, Sukru
Bu çalışmanın amacı, sezon dışı dönemde değişik oranlardaki α-tokoferolün koç spermasının dondurulabilirliğine etkisini incelemektir. Çalışmada 4 adet Merinos koçu (2- 3 yaşlarında ve Bademli/Burdur’da özel bir işletmede) kullanıldı. Elektroejakülatör kullanılarak koçlardan alınan ejakulatlardan > %80 motilite ve 1.5 x109 spermatozoa/ml üzerinde yoğunluğu bulunan ejakulatlar birleştirildi. Birleştirilen ejakulat beş eşit gruba ayrılarak 0 mg (kontrol), 100 μM, 200 μM, 400μM or 800 μM α-tokoferol içeren tris ile sulandırıldı, 4 C⁰’de ekibrilasyon yapılarak mini payetlerde donduruldu ve sıvı azotta depo edildi. Payetler daha sonda spermatolojik analizler için 37 C⁰’de 30 sn çözdürüldü. Kontrol grubuna (37.50±7.90; 26.85±3.17) göre sırası ile en yüksek motilite ve membran bütünlüğü 100 μM grupta (47.77±4.40; 34.83±2.64) tespit edildi (p<0.05). Ayrıca gruplar arası anormal sperma muayene sonuçlarında istatistiksel bir farklılık bulunmadı (p>0.05). Sonuç olarak, 100 μM α-tokoferol koç sperma dondurma sulandırıcılarına kontrol grubu ile karşılaştırıldığında sezon dışında motilite ve membran bütünlüğünü iyileştirdiği için eklenmelidir.
Show more [+] Less [-]Sub-Lethal Effects of Heavy Metals Toxicity on Pathological Lesions of Sea Bream
2018
Hedayati, Aliakbar
Histopathological indices have been largely used as biomarkers in the monitoring of fish health status during exposure to heavy metals, both inthe experimental and environmental studies. The aims of the present study was to provide baseline data on the prevalence of histopathologicalliver lesions in marine fishes (case study of liver histopathology at mercury exposure) under experimental mercury exposure and to comparethe sampling areas in terms of the types and prevalence of lesions present, for monitoring purposes. Experimental study was at seawaterre-circulatory tanks. Mercury concentrations were determined using a standard cold vapor atomic absorption. Histopathological analyses weredone in tissue processor and the slides were stained with haematoxylin and counter stained with eosin. There were many liver lesions in botharea include enlarged and lateral nuclei, nuclear degeneration and vacuolation; oncotic, apoptic, focal, massive, centrilobular and periportalnecrosis; atrophy, lipidosis, hydropic and cloudy swelling, oval cell proliferation; bile stagnation, dilation of sinusoid, intracellular edemaand dark granules. In conclusion the present investigation indicated that mercury is a toxic substance in seabream and the sub-lethal mercuryconcentrations tested may cause several changes in the histological indices of the studied fish and we can use these changes as biomarkers ofmercury detection.
Show more [+] Less [-]Occurrence of the Burrowing goby Trypauchen vagina (Bloch and Schneider, 1801) in Southeastern Mediterranean, Turkey
2018
ERGÜDEN, Deniz | KABAKLI, Ferhat | ALAGÖZ ERGÜDEN, Sibel | ALTUN, Ayhan
A single male specimen of the burrowing goby, Trypauchen vagina (Bloch and Schneider, 1801), was caught at a depth of 30 m from the Arsuz coast, Turkey by a trammel net on 17 October 2017. Total length (TL) of the specimen measured as 206 mm. Measurements of the specimen were given and the geographical distribution of the species in the Mediterranean was documented. Although this is the third record of T. vaginafrom the Mediterranean coast of Turkey, the paper is the first report of an adult male specimen of T. vagina from southern coast of Turkey and confirms the extension of the species towards southeastern Mediterranean in the region. The finding of T. vagina in Arsuz coast suggests the habitat expansion of the species. As a result of this study a gap is filled relating to the geographic distribution of this species in the easternMediterranean coast of Turkey.
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