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GENOTYPING OF HOLSTEIN FRIESIAN CROSSBRED CATTLE FOR CVM AND FXI DEFICIENCY LOCI
2024
S.B. Khade | V.D. Pawar | A.Y. Doiphode | M.P. Sawane | U.D. Umrikar
In animal breeding, genetic disorders are one of the most important issues for breeders. The present study was carried out to genotype HF crossbred cattle for FXI deficiency and CVM using PCR and PCR-RFLP using 50 blood samples. Amplicons of 244 bp for FXI gene and the 287 bp for SLC35A3 gene were successfully amplified. Analysis of amplicons of FXI gene revealed single 244 bp fragment which indicates absence of mutant allele. The amplicons of 287 bp of SLC35A3 gene was digested with Pst I RE which revealed two fragments of size 264 bp and 23 bp for normal animals. In the present study, out of fifty HF crossbred animals screened neither carrier nor affected animals were found for FXID and CVM.
Show more [+] Less [-]MACROANATOMY OF HIPPOCAMPUS IN BUFFALOES (BUBALUS BUBALIS)
2024
A. Kumaravel | Geetha Ramesh | S. Rajathi | S. Muthukrishnan | V. Ramakrishnan
The gross studies on the hippocampus were made on 10 specimens each of calves and adults of the Indian buffalo. The hippocampus was a curved elevation, lying immediately ventral to the splenium of the corpus callosum, dorsal to the lateral aspect of the thalamus. It occupied the floor of the inferior horn of the lateral ventricle. The ventricular surface of the hippocampus was covered by a thin layer of white matter, the alveus. Rostrolaterally the alveus joined a thick fibrous band, the hippocampal fimbria which connected the alveus to the fornix. On the ventromedial aspect, a deep groove, the hippocampal sulcus, separated this surface into lateral and medial portions.
Show more [+] Less [-]PARACETAMOL POISONING IN A CAT AND ITS TREATMENT
2024
P. Pothiappan | T. Muthuramalingam | R. Sureshkumar | G. Selvakumar | M. Thangapandiyan | G. D. Rao
A two year old male cat was presented to the Emergency and Critical Care Unit with a history of inappetence after paracetamol 300 mg (Half tablet of 600 mg) administration overnight. On clinical examination the cat had a mild elevation of vital signs. Based on history and clinical signs the case was diagnosed as paracetamol poisoning and treated with activated charcoal a total dose of 3 g)orally and N-acetyl cysteine intravenously at 140 mg/kg along with Ringer’s lactate, 10 ml/kg b.wt. The owner was advised to administer Nacetyl cysteine at 70 mg/kg orally for every 6 hours for five times. After treatment the cat was recovered successfully.
Show more [+] Less [-]PERFORMANCE AND INTESTINAL INTEGRITY OF BROILER CHICKENS BY SUPPLEMENTATION OF YEAST SACCHAROMYCES BOULARDII THROUGH WATER
2024
K. Naga Raja Kumari | T. Susmita
The study was carried out to evaluate the effect of Saccharomyces boulardii (Sb) on performance and intestinal integrity in broilers. A total of 300 day old Vencobb chicks were randomized in 5 groups, each with 6 replications (5x6) 10 birds per replicate. Groups provided with Sb ( 2 × 108 cfu/kg) in 0,0.02,0.04,0.08 and 0.16 mg/L in D1, D2, D3, D4, D5 respectively through water up to 42 days of age with adlibitum isonitrogenous and isocaloric ration. The results revealed, a significant (p<0.05) improvement in body weight gain, better FCR with increased concentration of Sb in water (D5). No significant variation was observed in feed intake with the level of Sb in water. Intestinal villus height, cryptal depth increased in groups with increased Sb. The results suggest that supplementation of Sb at 0.16mg/L through drinking water improved the performance of the broilers by increasing the absorption capacity in the gut.
Show more [+] Less [-]PATTERN OF SHEEP MIGRATION IN SOUTHERN TAMIL NADU
2024
A. Singaravadivelan | N. Kumaravelu | T. Sivakumar | S.M.K. Karthickeyan
A study was conducted to analyse the migratory sheep production system in southern agro-climatic zone of Tamil Nadu. A total of 100 migratory sheep farmers were randomly selected for the study and divided as small (< 175), medium (175- 350) and large (> 350) flocks based on the average flock size for comparison. The migratory tracts were identified based on the results of the enquiry from the sheep farmers, animal husbandry department personnel and by direct identification. A total number of 78 migratory tracts comprising 28 major and 50 minor tracts were identified in the study area. The mean radial distance (km) of migration in major tracts was 317.00±6.21 and minor tract was 148.40±6.21.
Show more [+] Less [-]INFLUENCE OF SPROUTED GREEN GRAM, SELENIUM AND VITAMIN-E AND THEIR COMBINATIONS ON REPRODUCTIVE PERFORMANCE OF WHITE LEGHORN MALES
2024
R. Karthika | M. Moorthy | S.C. Edwin | K. Mani | S.Panneerselvam
An experiment was conducted to study the influence of sprouted green gram, selenium and vitamin E and their combinations on reproductive performance like semen quality and fertility for a period of five weeks with forty two adult White Leghorn cocks and seventy White Leghorn hens which were divided into seven treatment groups containing six cocks and ten hens for each treatment. The treatment groups consisted of control (T1), sprouted grains 50g/kg (T2), selenium 0.5mg/kg (T3), vitamin E 500mg/kg (T4), sprouted grains 50g/kg + selenium 0.5mg/kg (T5), sprouted grains 50g/kg + vitamin E 500mg/kg (T6) and sprouted grains 50g/kg + selenium 0.5mg/kg + vitamin E 500mg/kg (T7). Significant (P<0.05) difference was observed between treatment groups. The overall sperm motility of treatment and control groups did not differ significantly. The sperm concentration was significantly (P<0.05) higher in all treatment groups compared to control. Inclusion of sprouted green gram, selenium and vitamin E had significantly reduced the overall dead (P<0.01) and abnormal (P<0.05) sperms when compared to the control group.
Show more [+] Less [-]EFFECT OF FROZEN STORAGE ON THE PHYSICO-CHEMICAL QUALITY AND HISTOLOGY OF QUAIL BREAST MEAT
2024
O.V.Sonale | A.M. Chappalwar | A.A. Devangare
This experiment was carried out to study the effect of frozen storage on the physico-chemical quality of quail breast meat. Six weeks old broiler quails were collected, slaughtered, dressed hygienically. Quail breast meat samples were collected from dressed carcass which was packed in low density polyethylene pouches taking optimum care to avoid contamination and stored at -18 ± 20 C for 60 days. Fresh as well as frozen quail breast meat samples were evaluated for physico-chemical and histological changes. Frozen meat samples were analyzed after thawing at 4 ± 10 C for 12 hrs at an interval of 15, 30, 45 and 60 days. It was observed that with advancement of the frozen storage period pH, TBA value, tyrosine value and drip loss increased, whereas moisture, protein, fat and ERV content of quail meat decreased. Pronounced structural changes reported at later part of storage in histological study due to formation of ice crystals in the muscles. It shows that quail meat can be safely stored for 60 days in frozen state without any marked changes.
Show more [+] Less [-]CHOCOLATE TOXICITY IN A DOG
2024
A.R. Ahlawat | S.N. Ghodasara | V.B. Dongre | P. U. Gajbhiye
Animals of all ages are susceptible for chocolate toxicity affecting many organ systems. The case presented here is of male German shepherd aged 4 years was accidentally encountered with chocolate toxicity with excessive salivation, vomition, diarrhoea, restlessness and incoordination like systems. The dog was treated symptomatically using rehydrated fluids, atropine sulphate and dexamethasone along with other supportive medication immediately. The dog was recovered completely within week of post treatment.
Show more [+] Less [-]Managing zoonotic infectious diseases in Africa: The key role approach
2024
Abdalla A. Latif
No abstract available.
Show more [+] Less [-]Evaluation of commercial ELISA kits’ diagnostic specificity for FAST diseases in wild animals
2024
Vesna Milićević | Dimitrije Glišić | Ljubiša Veljović | Jovan Mirčeta | Branislav Kureljušić | Milutin Đorđević | Nikola Vasković
Wild animals, sharing pathogens with domestic animals, play a crucial role in the epidemiology of infectious diseases. Sampling from wild animals poses significant challenges, yet it is vital for inclusion in disease surveillance and monitoring programmes. Often, mass surveillance involves serological screenings using enzyme-linked immunosorbent assay (ELISA) tests, typically validated only for domestic animals. This study assessed the diagnostic specificity of commercially available ELISA tests on 342 wild ruminant serum samples and 100 from wild boars. We evaluated three tests for foot-and-mouth disease: two for Peste des petits ruminants, two for Rift Valley fever and one for Capripox virus. Diagnostic specificity was calculated using the formula True Negative/(False Positive + True Negative). Cohen’s kappa coefficient measured agreement between tests. Results showed high specificity and agreement across all tests. Specificity for foot-and-mouth disease (FMD) ranged from 93.89% for Prionics to 100% for IDEXX, with IDvet showing 99.6%. The highest agreement was between FMD IDvet and IDEXX at 97.1%. Rift Valley fever (RVF) tests, Ingezim and IDvet, achieved specificities of 100% and 98.83%, respectively. The optimal specificity was attained by retesting single reactors and inactivating the complement. Contribution: Commercially available ELISA kits are specific for foot-and-mouth disease and similar transboundary animal diseases and can be used for highly specific wild animal testing.
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