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Molecular characteristics of fowl adenovirus strains detected in broiler chickens on diets without immunostimulant supplements Full text
2024
Niczyporuk Jowita Samanta | Kozdruń Wojciech | Tomczyk Grzegorz | Piekarska Karolina | Barabasz Marcin | Michalski Marcin
Outbreaks of fowl adenovirus (FAdV) infection in chicken flocks in Poland threaten birds’ health and lives and are rising in frequency. The risk of these infections in immunocompromised poultry flocks with developed clinical symptoms was analysed through virus detection in broiler chicks and correlation of cases with the birds’ immune strength.
Show more [+] Less [-]Chickens’ eggs and the livers of farm animals as sources of perfluoroalkyl substances Full text
2024
Mikołajczyk Szczepan | Warenik-Bany Małgorzata | Pajurek Marek
This study focuses on perfluoroalkyl substance (PFAS) content in chickens’ eggs and the livers of farm animals.
Show more [+] Less [-]Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses Full text
2024
Olech Monika | Kuźmak Jacek
Small ruminant lentiviruses (SRLV) cause multisystemic, degenerative and chronic disease in sheep and goats. There are five genotypes (A, B, C, D and E), of which A and B are the most widespread. The purpose of this study was to evaluate the serotyping efficiency of the Eradikit SRLV Genotyping ELISA and the molecular typing efficiency of a newly developed nested real-time PCR targeting the long terminal repeat–gag (LTR-gag) region using samples from animals infected with subtypes of SRLV known to circulate in Poland.
Show more [+] Less [-]Occurrence of herpesvirus in fish Full text
2024
Bergmann, Sven Michael | Wang, Yingying | Li, Yingying | Wang, Qing | Klafack, Sandro | Jin, Yeonhwa | Hofmann, Arndt Christian | Kielpinska, Jolanta | Becker, Anna Maria | Zeng, Weiwei
Occurrence of herpesvirus in fish Full text
2024
Bergmann, Sven Michael | Wang, Yingying | Li, Yingying | Wang, Qing | Klafack, Sandro | Jin, Yeonhwa | Hofmann, Arndt Christian | Kielpinska, Jolanta | Becker, Anna Maria | Zeng, Weiwei
Herpesviruses are common agents in animals of the aquatic environment. They infect many species of fish but only lead to disease in one or two species. Nevertheless, infected fish without clinical symptoms can actively transfer infectious agents to disease-susceptible species. The aim of the study was to identify and prove the natural presence of different herpesviruses. Material and Methods Koi, Nile tilapia, grass carp, goldfish and crucian carp were infected with a herpesvirus isolate 99% identical to goldfish herpesvirus (GHV) or cyprinid herpesvirus 2 (CyHV-2) obtained from crucian carp. Before and after infection, samples were collected non-lethally at different time points from all five fish species to identify and evaluate the replication of viruses naturally infecting the fish as well as the CyHV-2 experimentally infecting them. Gill swabs and separated leukocytes were subjected to PCR and the results compared. Results These samples yielded DNA of koi herpesvirus (KHV, also referred to as CyHV-3), GHV and a new herpesvirus. While Asian-lineage CyHV-3 DNA was detected in samples from crucian carp and goldfish, CyHV-2 DNA was found in samples from koi and tilapia. A new, hitherto unknown herpesvirus was identified in samples from grass carp, and was confirmed by nested PCR and sequence analysis. The survival rates were 5% for grass carp, 30% for tilapia, 55% for crucian carp, 70% for koi and 100% for goldfish at 20 days post infection. Evolutionary analyses were conducted and five clusters were visible: CyHV-1 (carp pox virus), CyHV-2 with sequences from koi and tilapia, CyHV-3 with sequences from crucian carp and goldfish, probable CyHV-4 from sichel and a newly discovered herpesvirus – CyHV-5 – from grass carp. Conclusion The results obtained with the molecular tools as well as from the animal experiment demonstrated the pluripotency of aquatic herpesviruses to infect different fish species with and without visible clinical signs or mortality.
Show more [+] Less [-]Occurrence of herpesvirus in fish Full text
2024
Bergmann Sven Michael | Wang Yingying | Li Yingying | Wang Qing | Klafack Sandro | Jin Yeonhwa | Hofmann Arndt Christian | Kielpinska Jolanta | Becker Anna Maria | Zeng Weiwei
Herpesviruses are common agents in animals of the aquatic environment. They infect many species of fish but only lead to disease in one or two species. Nevertheless, infected fish without clinical symptoms can actively transfer infectious agents to disease-susceptible species. The aim of the study was to identify and prove the natural presence of different herpesviruses.
Show more [+] Less [-]Assessing tropism and genetic traits of carp oedema virus isolates to enhance detection strategies Full text
2024
Adamkowska, Natalia | Kiełpińska, Jolanta | Bergmann, Sven Michael
Assessing tropism and genetic traits of carp oedema virus isolates to enhance detection strategies Full text
2024
Adamkowska, Natalia | Kiełpińska, Jolanta | Bergmann, Sven Michael
Introduction Carp oedema virus (CEV) is a relatively understudied poxvirus. It exhibits an affinity for gill and skin epithelial cells. Investigations were conducted into selected aspects of CEV biology, with a focus on determining cell and tissue tropism of CEV, acquiring gene sequences and updating CEV tests in fish tissues. Material and Methods A total of 238 common carp tissue samples from nine aquaculture farms were analysed. The study evaluated the efficacy of intermediate detection of CEV by real-time PCR and in situ hybridisation. The genes encoding protein P4a were sequenced, analysed and aligned in a phylogenetic tree using a molecular evolution model. Results In situ hybridisation revealed the necessity to validate the Centre for Environment, Fisheries and Aquaculture Science protocols for sampling for CEV detection and to use the tissues for which the virus has the highest tropism, namely the skin and kidneys, rather than solely the gills. The level of genetic variability was determined, and it was shown that CEV mutates systematically. The creation of two distinct phylogenetic clades confirms certain strains’ description as Polish isolates. Conclusion Determining the localisation of CEV genetic material in organs and tissues is pivotal for shaping the World Organisation for Animal Health guidelines. The utility of molecular diagnostics has been demonstrated in the skin and kidney of carp, in addition to the gills, impelling their inclusion in diagnostic protocols. The clusters identified in the phylogenetic tree offer valuable insights for developing the current PCR primers. The prevalence of CEV infection in aquaculture, juxtaposed with its notably lower detection in wild fish, underscores the significance of mandatory molecular diagnostic testing for CEV in carp farming.
Show more [+] Less [-]Assessing tropism and genetic traits of carp oedema virus isolates to enhance detection strategies Full text
2024
Adamkowska Natalia | Kiełpińska Jolanta | Bergmann Sven Michael
Carp oedema virus (CEV) is a relatively understudied poxvirus. It exhibits an affinity for gill and skin epithelial cells. Investigations were conducted into selected aspects of CEV biology, with a focus on determining cell and tissue tropism of CEV, acquiring gene sequences and updating CEV tests in fish tissues.
Show more [+] Less [-]A Retrospective Study on The Evaluation of Hind Limb Bone Fractures in Cats Full text
2024
Canlı, Rojda | Çatalkaya, Emine | Ersöz Kanay, Berna | Saylak, Nahit | Kilinç, Mehmet | Altan, Semih | Yayla, Sadık
As a result of many traumatic factors such as traffic accidents, falls from height, gunshot wounds and animal fights with each other, orthopedic and soft tissue damage, especially fractures, occur in the musculoskeletal system. Among the fractures within these lesions, the incidence of hind limb long bone fractures is higher than other bones. While the incidence of femur fractures among other fractures in cats is 20-26%, tibia fractures constitute a significant proportion of approximately 20% of other fractures. This study aimed to retrospectively evaluate the distribution, etiology, treatment, and treatment results of fractures in cats with hind limb bone fractures (n=92). In addition to the use of intramedullary nails and plates, external fixators are also used for the treatment of hind limb bone fractures. Despite the advantages and disadvantages of each technique, most of the time no problems are observed during the application of these techniques and in the postoperative period. A functional recovery was observed in all cats at postoperative follow-up. As a result, it can be said that the method to be used in hind limb bone fractures in cats varies according to the surgeon's preference, experience, and existing orthopedic material inventory. However, anatomical locking plates have the advantages of not using postoperative bandages and allowing the limb to be used early.
Show more [+] Less [-]Renal Histopathology and Serum Biochemistry of Cadmium-exposed Rabbit Bucks Administered Methanolic Extract of Phoenix dactylifera Fruit Full text
2024
Ansa, Anietie | Akpere, Odamesan
Cadmium (Cd), a prevalent heavy metal pollutant, is known for its harmful effects on physiological functions within the biological system. In this study, the effects of Cd exposure on some kidney related biochemical and histopathological parameters in rabbits were investigated, alongside exploring the potential protective role of methanolic extract from Phoenix dactylifera (MEPD) on the kidney. The study included 45 male rabbits, aged 24-28 weeks and weighing approximately 1.41-1.43 kg were assigned into five (5) treatment groups (control, Cd-only, Cd + 300 mg MEPD, Cd + 600 mg MEPD and Cd + 900 mg MEPD) in a completely randomized design. The rabbits received a 7-day dose of 3 mg CdCl2/kg feed, followed by MEPD every 72 hours for 28 days. Results revealed higher significant (P<0.05) differences in serum glucose, urea and creatinine in Cd-only treated group when compared with the control group. MEPD treatment significantly (P<0.05) lowered serum glucose levels in Cd-exposed rabbits, indicating a potential glycemic modulating activity, but had no significant (P>0.05) effect on urea and creatinine concentrations. High density lipoprotein (HDL), low-density lipoprotein (LDL) and cholesterol ratios were not significantly different but showed marginal improvement in the Cd+MEPD groups compared to the Cd-only group. The findings also revealed significant differences in both absolute and relative kidney weights between the group treated with Cd alone and the control group, indicating potential renal injury induced by Cd. Histopathological examination revealed kidney damage in Cd-exposed rabbits, with observed changes such as disruption of Bowman’s capsule, glomerular shrinkage, degeneration of renal tubules and haemorrhage. The administration of MEPD did not ameliorate the observed histopathological alterations. In conclusion, this study suggests that Cd exposure may induce hyperglycemia and kidney injury in rabbits. MEPD treatment demonstrated some protective effects against Cd-induced hyperglycemia but did not significantly mitigate kidney damage.
Show more [+] Less [-]Evaluation of Medetomidine Alone or in Combination with Tramadol on Tear Secretion in Cats and Their Reversal with Atipamezole Full text
2024
Caner, Esma Tuğçe | Okur, Sıtkıcan
To assess the effect of administration of medetomidine alone or in combination with tramadol on tear secretion (TS) in cats as well as their reversal with atipamezole. For the purpose of the study, a total of 46 cats, representing different breeds and genders, were selected and divided into two groups using a random assignment method. Group M was administered medetomidine at a dose of 80 µg/kg intramuscularly. Group MT was given a combination of medetomidine and tramadol at doses of 80 µg/kg and 2 mg/kg intramuscularly, respectively. Tear secretion was measured using Schirmer tear test I before sedation and at 15 (T15) - 60 (T60) minutes post-sedation with 15 min intervals. At 30 minutes, all cats were given atipamezole (200 µg/kg IM). TS statistically decreased until T30 measurement in both groups (P < 0.05). The TS decreased more in MT group compared to M group at T30 measurements (P < 0.05). TS increased in both groups post-atipamezole but didn't return to initial (T0) levels by study end (T60). Premedication with tear protectors or artificial tears is advised when using MT and M group agents in cats, and atipamezole can reverse their effects post-procedure.
Show more [+] Less [-]Poisoning Plants in Cats and Dogs Full text
2024
Dural, Ebru | Ince, Sinan
Today, cats and dogs are common pets among domestic animals. These animals are exposed to many diseases or poisonings in their lives. In cases of poisoning, plants have an important role. In this respect, it should not be forgotten that plants at home or outside can cause poisoning in animals. In this review, some plants that can pose a potential danger to cats and dogs, their properties, and the treatment options that can be made with clinical signs in case of poisoning are mentioned.
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