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The Role of Several External Factors on Controlling Rabies In The City of Medan Full text
2019
Rasmaliah, Rasmaliah | Hiswani, Hiswani | Lubis, Sri Novita
The Role of Several External Factors on Controlling Rabies In The City of Medan Full text
2019
Rasmaliah, Rasmaliah | Hiswani, Hiswani | Lubis, Sri Novita
Rabies cases are widespread worldwide and mostly prevalence in develeloping countries. Several Province in Indonesia are still categorized as endemic rabies, including the Province of North Sumatera. This research was aimed to evaluate the role of external factors that could influence in reduction the rabies suspected cases in the population of the City of Medan. The survey was conducted using a structured questionnaire. A Case-Control Design was used in this study. The two groups were rabies suspected case and rabies unsuspected case (control), consisted both male and female respondents. All the population involved in this study were living in the City of Medan. Primary and secondary data were collected usingpurposive samplingapproach. Data were analysed by univariate analysis, bivariate analysis, and multivariate analysis. The result was obtained the ratio of 1:2 between rabies suspected case (64 cases) and rabies unsuspected case (128 cases) in the City of Medan. The bivariate analysis showed the significant influence of the role of livestock department officer, role of health worker, role of community leader and vaccination tools on the rabies suspected case (P0.05). Multivariate analysis showed that the dominant variable which affect the rabis suspected case was rabies vaccine tools.
Show more [+] Less [-]The Role of Several External Factors on Controlling Rabies In The City of Medan Full text
2019
Rasmaliah Rasmaliah | Hiswani Hiswani | Sri Novita Lubis
Rabies cases are widespread worldwide and mostly prevalence in develeloping countries. Several Province in Indonesia are still categorized as endemic rabies, including the Province of North Sumatera. This research was aimed to evaluate the role of external factors that could influence in reduction the rabies suspected cases in the population of the City of Medan. The survey was conducted using a structured questionnaire. A Case-Control Design was used in this study. The two groups were rabies suspected case and rabies unsuspected case (control), consisted both male and female respondents. All the population involved in this study were living in the City of Medan. Primary and secondary data were collected using purposive sampling approach. Data were analysed by univariate analysis, bivariate analysis, and multivariate analysis. The result was obtained the ratio of 1:2 between rabies suspected case (64 cases) and rabies unsuspected case (128 cases) in the City of Medan. The bivariate analysis showed the significant influence of the role of livestock department officer, role of health worker, role of community leader and vaccination tools on the rabies suspected case (P0.05). Multivariate analysis showed that the dominant variable which affect the rabis suspected case was rabies vaccine tools.
Show more [+] Less [-]What Constitutes Effective Interprofessional Education Amongst Health Related Disciplines : A Literature Review Full text
2019
Wardani, Elly | Rachmah, R. | Mayasari, Putri | Mahdarsari, Mayanti
What Constitutes Effective Interprofessional Education Amongst Health Related Disciplines : A Literature Review Full text
2019
Wardani, Elly | Rachmah, R. | Mayasari, Putri | Mahdarsari, Mayanti
Interprofessional Education (IPE)is considered an educational strategy that has potential benefit forimproving teamwork and collaboration in practice, leading to improved patient outcomes. As the development and implementation of curricula that integrate IPE requires significant resources, its adoption should be based on evidence of effectiveness. The purpose of this literature review is to identify what constitutes effective interprofessional education amongst health related disciplines. A literature search was conducted using Science Direct, BMC, Elsevier and Joint Commission Journal for the years 2009-2018. A total of 15 studies met the established inclusion criteria. The use of IPE among health related disciplines is one effective way to solve several problems, and its particularly improves patient safety. What constitutes effective interprofessional education amongst health related disciplines are possibly viewed from some aspects, such as efficiency, effectiveness, learning strategy, power, patient and learner safety and readiness to practice, interprofessional curriculum, roles and relationships, and learning outcomes.
Show more [+] Less [-]What Constitutes Effective Interprofessional Education Amongst Health Related Disciplines : A Literature Review Full text
2019
Elly Wardani | R. Rachmah | Putri Mayasari | Mayanti Mahdarsari
Interprofessional Education (IPE) is considered an educational strategy that has potential benefit for improving teamwork and collaboration in practice, leading to improved patient outcomes. As the development and implementation of curricula that integrate IPE requires significant resources, its adoption should be based on evidence of effectiveness. The purpose of this literature review is to identify what constitutes effective interprofessional education amongst health related disciplines. A literature search was conducted using Science Direct, BMC, Elsevier and Joint Commission Journal for the years 2009-2018. A total of 15 studies met the established inclusion criteria. The use of IPE among health related disciplines is one effective way to solve several problems, and its particularly improves patient safety. What constitutes effective interprofessional education amongst health related disciplines are possibly viewed from some aspects, such as efficiency, effectiveness, learning strategy, power, patient and learner safety and readiness to practice, interprofessional curriculum, roles and relationships, and learning outcomes.
Show more [+] Less [-]Characterization Evaluation of Clove Flower Oil (Syzygium aromaticum (L.) Meer) And Pharmacological Properties Of Anthelmintic Full text
2019
Utami, Cut Suraiya Wahyuni | Balqis, Ummu | Athaillah, Farida | Hanafiah, Muhammad | Iskandar, Cut Dahlia
Characterization Evaluation of Clove Flower Oil (Syzygium aromaticum (L.) Meer) And Pharmacological Properties Of Anthelmintic Full text
2019
Utami, Cut Suraiya Wahyuni | Balqis, Ummu | Athaillah, Farida | Hanafiah, Muhammad | Iskandar, Cut Dahlia
Clove oil is distilled from clove flowers (Syzigium aromaticum) and characterized by physical properties and GC-MS. Distillation was carried out by steam distillation method for 8 hours. Analysis of clove oil was carried out by GC-MS method. The distillation results obtained 163 mL of clove oil with physical properties that meet the requirements of SNI 06-4267-1996. The results of the analysis using GC-MS showed that clove oil contained 6 components, namely, Eugenol 88.20%, Alpha Copaene 0.49%, Caryophyllene 3.77%, Alpha Humulene 0.48%, Eugenyl acetate 6.76%, and Caryophyllene Oxide 0.30%. Another fact shows that clove flower oil has anthelmintic activity.
Show more [+] Less [-]Characterization Evaluation of Clove Flower Oil (Syzygium aromaticum (L.) Meer) And Pharmacological Properties Of Anthelmintic Full text
2019
Cut Suraiya Wahyuni Utami | Ummu Balqis | Farida Athaillah | Muhammad Hanafiah | Cut Dahlia Iskandar
Clove oil is distilled from clove flowers (Syzigium aromaticum) and characterized by physical properties and GC-MS. Distillation was carried out by steam distillation method for 8 hours. Analysis of clove oil was carried out by GC-MS method. The distillation results obtained 163 mL of clove oil with physical properties that meet the requirements of SNI 06-4267-1996. The results of the analysis using GC-MS showed that clove oil contained 6 components, namely, Eugenol 88.20%, Alpha Copaene 0.49%, Caryophyllene 3.77%, Alpha Humulene 0.48%, Eugenyl acetate 6.76%, and Caryophyllene Oxide 0.30%. Another fact shows that clove flower oil has anthelmintic activity.
Show more [+] Less [-]Lactic Acid Bacteria and Histamine Levels of Sie Balu After Gamma Irradiated. Full text
2019
Arham, Arham | Nurliana, Nurliana | Sugito, Sugito
Lactic Acid Bacteria and Histamine Levels of Sie Balu After Gamma Irradiated. Full text
2019
Arham, Arham | Nurliana, Nurliana | Sugito, Sugito
The presence of lactic acid bacteria (LAB) and histamine in foodstuffs indicate the level of deterioration in the quality of food and cause poisoning.Sie Baluis the Acehnese dried meat preserved by the addition of salt, acid and dried, but the long processing and drying it under the sun can cause microbial contamination in meat products. Irradiation can eliminate bacteria in foodstuffs. This study aimed to determine the amount of LAB and histamine levels ofSie Baluafter irradiation doses of 5, 7 and 9 kGy and stored 2 to 4 months.Sie Baluwas made of fresh beef 5 kg, dried in the sun to dry, vacuumed and irradiated with gamma rays. The samples for LAB determination cultured in MRS agar and incubated at 37C for 24 hours. The number of colonies was counted using Total Plate Count. The histamine level ofSie Baluconducted by ELISA. Irradiation did not significantly (P0.05) affect the amount of LAB, but the shelf life significantly (P0.05) affected the amount of LAB inSie Balu. Extending the shelf life up to 4 months can increase the amount of LAB. Irradiation dose and shelf life had no effect on histamine levels ofSie Balu(P0.05). This study concluded that irradiatedSie Balucannot be stored for more than two months.
Show more [+] Less [-]Lactic Acid Bacteria and Histamine Levels of Sie Balu After Gamma Irradiated. Full text
2019
Arham Arham | Nurliana Nurliana | Sugito Sugito
The presence of lactic acid bacteria (LAB) and histamine in foodstuffs indicate the level of deterioration in the quality of food and cause poisoning. Sie Balu is the Acehnese dried meat preserved by the addition of salt, acid and dried, but the long processing and drying it under the sun can cause microbial contamination in meat products. Irradiation can eliminate bacteria in foodstuffs. This study aimed to determine the amount of LAB and histamine levels of Sie Balu after irradiation doses of 5, 7 and 9 kGy and stored 2 to 4 months. Sie Balu was made of fresh beef 5 kg, dried in the sun to dry, vacuumed and irradiated with gamma rays. The samples for LAB determination cultured in MRS agar and incubated at 37°C for 24 hours. The number of colonies was counted using Total Plate Count. The histamine level of Sie Balu conducted by ELISA. Irradiation did not significantly (P0.05) affect the amount of LAB, but the shelf life significantly (P0.05) affected the amount of LAB in Sie Balu. Extending the shelf life up to 4 months can increase the amount of LAB. Irradiation dose and shelf life had no effect on histamine levels of Sie Balu (P0.05). This study concluded that irradiated Sie Balu cannot be stored for more than two months.
Show more [+] Less [-]Antibiotic Activities To Staphylococcus aureus Phospholipase Of Aceh Cattle Preputium Isolate Full text
2019
Dewi, Maryulia | Adhayani, Layli | Zuraidawati, Zuraidawati | Erina, Erina
Antibiotic Activities To Staphylococcus aureus Phospholipase Of Aceh Cattle Preputium Isolate Full text
2019
Dewi, Maryulia | Adhayani, Layli | Zuraidawati, Zuraidawati | Erina, Erina
This study aimed to study the effect of antibiotic on phospholipase production ofStaphylococcus aureusisolated from preputium of Aceh cattle. The parameters measured in this study were PzI ofStaphylococcus aureuswithout any treatment, and PzI ofS. aureusafter being given antibiotics. The experiment was carried out by modifying the Samaranayake method on egg yolk agar media, incubated at 37 C for 48 hours, and the precipitation lines formed was measured. The antibiotics used were tetracycline, oxytetracycline and fosfomycin with concentrations of 10%, 20% and 30% (mg / mL, respectively). The results showed that phospholipase production ofS. aureusisolated from preputium of Aceh cattle was suppressed, antibiotics were able to inhibit phospholipase production. However, the enzyme was still produced in positive category, with PzI = 0.287, the highest result was found in 30% tetracycline activity, which is equal to PzI = 0.341 and the lowest was in 10% fosfomycin which is equal to PzI = 0.332
Show more [+] Less [-]Antibiotic Activities To Staphylococcus aureus Phospholipase Of Aceh Cattle Preputium Isolate Full text
2019
Maryulia Dewi | Layli Adhayani | Zuraidawati Zuraidawati | Erina Erina
This study aimed to study the effect of antibiotic on phospholipase production of Staphylococcus aureus isolated from preputium of Aceh cattle. The parameters measured in this study were PzI of Staphylococcus aureus without any treatment, and PzI of S. aureus after being given antibiotics. The experiment was carried out by modifying the Samaranayake method on egg yolk agar media, incubated at 37 ° C for 48 hours, and the precipitation lines formed was measured. The antibiotics used were tetracycline, oxytetracycline and fosfomycin with concentrations of 10%, 20% and 30% (mg / mL, respectively). The results showed that phospholipase production of S. aureus isolated from preputium of Aceh cattle was suppressed, antibiotics were able to inhibit phospholipase production. However, the enzyme was still produced in positive category, with PzI = 0.287, the highest result was found in 30% tetracycline activity, which is equal to PzI = 0.341 and the lowest was in 10% fosfomycin which is equal to PzI = 0.332
Show more [+] Less [-]Acute-phase protein profile in horses subjected to different exercise protocols Full text
2019
Assuncao, P. | Barbosa, T. | Yonezawa, L. | Barbosa, L. | Watanabe, M. | Kohayagawa, A. | Schmidt, E.
High-intensity exercise can be associated with the occurrence of muscle injury, as well as the induction of an acute-phase response (APR). The present study aims to investigate the synthesis and profile of serum proteins in horses before and after participating in 2 different exercise protocols and to relate this profile to the presence or absence of muscular injury caused by exercise. Ten purebred Arabian (n = 5) and Criollo (n = 5) horses were subjected to 2 different tests on a treadmill, one consisting of short-duration and rapid-acceleration training (TRA) that was mostly anerobic and the other of long-duration and slow-acceleration training (TLD) that was predominantly aerobic. Blood samples were obtained before the beginning of exercise (T0) and at 6 post-exercise time points: immediately after (T1) and 30 min (T2), 3 h (T3), 12 h (T4), 24 h (T5), and 48 h (T6) after exercise. Hematocrit was determined by the microhematocrit method. Plasma and serum samples were prepared by centrifugation (1500 × g for 5 min) for plasma concentrations of fibrinogen, total serum proteins (TP), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and creatine-kinase (CK) serum activity. Total protein concentration and CK serum activity were determined in an automated biochemistry analyzer. Fibrinogen was determined by the heat precipitation method in microhematocrit capillary tubes. Estimated concentrations of haptoglobin (Hp) significantly decreased after TRA and estimated concentrations of alpha-1 acid glycoprotein (AGP) significantly increased after both protocols at T2. Albumin increased after the TLD exercise protocol. Changes in hematocrit, haptoglobin, and albumin concentrations in horses subjected to different treadmill exercise protocols are related to a physiological response to hemoconcentration and hemolysis. Increases of AGP in the TLD protocol suggest the release of catecholamines as a response to avoid oxidative damage to tissue.
Show more [+] Less [-]Effect of topical ophthalmic administration of 0.005% latanoprost solution on aqueous humor flow rate and intraocular pressure in ophthalmologically normal adult Beagles Full text
2019
Fentiman, Katelyn E. | Rankin, Amy J. | Meekins, Jessica M. | Roush, James K.
OBJECTIVE To determine the effect of topical ophthalmic administration of 0.005% latanoprost solution on aqueous humor flow rate (AHFR) and intraocular pressure (IOP) in ophthalmologically normal dogs. ANIMALS 12 adult Beagles. PROCEDURES In a masked crossover design involving two 10-day experimental periods separated by a 7-day washout period, dogs were randomly assigned to first receive latanoprost or artificial tears (control) solution and then the opposite treatment in the later experimental period. Each experimental period was divided into a baseline phase (days 1 to 3), baseline fluorophotometry assessment (day 4), treatment phase (1 drop of latanoprost or artificial tears solution administered twice daily in each eye on days 5 to 9 and once on day 10), and posttreatment fluorophotometry assessment (day 10). Measured fluorescein concentrations were used to calculate baseline and posttreatment AHFRs. The IOP was measured 5 times/d in each eye during baseline and treatment (days 5 to 9) phases. RESULTS Mean baseline and posttreatment AHFR values did not differ significantly in either experimental period (latanoprost or control). In the latanoprost period, mean IOP was significantly lower during treatment than at baseline; there was no difference in corresponding IOP values during the control period. In the latanoprost period, mean IOP was significantly higher on the first day of treatment than on subsequent treatment days. CONCLUSIONS AND CLINICAL RELEVANCE In ophthalmologically normal dogs, topical ophthalmic administration of 0.005% latanoprost solution significantly decreased IOP but did not affect AHFR. Thus, the ocular hypotensive effect of latanoprost did not appear to have been caused by a reduction in aqueous humor production.
Show more [+] Less [-]Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods Full text
2019
Narita, Momoko | Nishida, Hidetake | Asahina, Ryota | Nakata, Kohei | Yano, Hirohito | Ueda, Tomoyuki | Inden, Masatoshi | Akiyoshi, Hideo | Maeda, Sadatoshi | Kamishina, Hiroaki
OBJECTIVE To compare ultracentrifugation, precipitation, and membrane affinity chromatography methods for isolation of extracellular vesicles (EVs) from canine plasma samples and to identify suitable reference genes for incorporation into a quantitative reverse transcription PCR assay of microRNA expression in plasma EVs of healthy dogs. ANIMALS 6 healthy Beagles. PROCEDURES Plasma samples were obtained from each dog, and EVs were isolated from 0.3 mL of these samples via ultracentrifugation, precipitation, and membrane-affinity chromatographic methods. Nanoparticle tracking analysis was performed to determine the concentration and size distribution of EVs isolated by the ultracentrifugation method. Expression levels (cycle threshold values) of 4 microRNAs (let-7a, miR-16, miR-26a, and miR-103) were then compared by means of quantitative reverse transcription PCR assay. Three statistical programs were used to identify the microRNAs most suitable for use as reference genes. RESULTS Results indicated that ultracentrifugation was the most stable of all 3 methods for isolating microRNAs from 0.3 mL of plasma. Nanoparticle tracking revealed that EV samples obtained by the ultracentrifugation method contained a mean ± SD of approximately 1.59 × 10(10) vesicles/mL ± 4.2 × 10(8) vesicles/mL. Of the 4 microRNAs in plasma EVs isolated by ultracentrifugation, miR-103 was the most stable. CONCLUSIONS AND CLINICAL RELEVANCE The ultracentrifugation method has potential as a stable method for isolating EVs from canine plasma samples with a high recovery rate, and miR-103 may provide the most stable reference gene for normalizing microRNA expression data pertaining to plasma EVs isolated by ultracentrifugation.
Show more [+] Less [-]Assessment of a commercially available veterinary blood pressure device used on awake and anesthetized dogs Full text
2019
Cremer, Jeannette | Da Cunha, Anderson F. | Paul, Linda J. | Liu, Qinqi | Acierno, Marc J.
OBJECTIVE To compare results of a commercially available device for oscillometrically measured blood pressure (OBP) with invasively measured blood pressure (IBP) in awake and anesthetized dogs. ANIMALS 19 adult dogs (mean ± SD body weight, 17.8 ± 7.5 kg). PROCEDURES Blood pressures were measured in dogs while they were awake and anesthetized with isoflurane. The OBP was recorded on a thoracic limb, and IBP was simultaneously recorded from the median caudal artery. Agreement between OBP and IBP was evaluated with the Bland-Altman method. Guidelines of the American College of Veterinary Internal Medicine (ACVIM) were used for validation of the oscillometric device. RESULTS In awake dogs, mean bias of the oscillometric device was −11.12 mm Hg (95% limits of agreement [LOA], −61.14 to 38.90 mm Hg) for systolic arterial blood pressure (SAP), 9.39 mm Hg (LOA, −28.26 to 47.04 mm Hg) for diastolic arterial blood pressure (DAP), and −0.85 mm Hg (LOA, −40.54 to 38.84 mm Hg) for mean arterial blood pressure (MAP). In anesthetized dogs, mean bias was −12.27 mm Hg (LOA, −47.36 to 22.82 mm Hg) for SAP, −3.92 mm Hg (LOA, −25.28 to 17.44 mm Hg) for DAP, and −7.89 mm Hg (LOA, −32.31 to 16.53 mm Hg) for MAP. The oscillometric device did not fulfill ACVIM guidelines for the validation of such devices. CONCLUSIONS AND CLINICAL RELEVANCE Agreement between OBP and IBP results for awake and anesthetized dogs was poor. The oscillometric blood pressure device did not fulfill ACVIM guidelines for validation. Therefore, clinical use of this device cannot be recommended.
Show more [+] Less [-]Anesthetic effects of dexmedetomidine-ketamine-midazolam administered intramuscularly in five-striped palm squirrels (Funambulus pennantii) Full text
2019
Eshar, David | Beaufrere, Hugues
OBJECTIVE To evaluate efficacy and safety of anesthesia with dexmedetomidine-ketamine-midazolam (DKM) in five-striped palm squirrels (Funambulus pennantii). ANIMALS 8 male squirrels. PROCEDURES Squirrels were anesthetized with DKM (dexmedetomidine, 0.1 mg/kg; ketamine hydrochloride, 30 mg/kg; and midazolam, 0.75 mg/kg) administered IM. Atipamezole (0.15 mg/kg) and flumazenil (0.1 mg/kg) were administered IM 40 minutes after induction of anesthesia. Vital signs and responses were recorded every 5 minutes during anesthesia. RESULTS Anesthetic induction and recovery from anesthesia were rapid and without complications in all squirrels. Median anesthetic induction time was 67.5 seconds (interquartile [25th to 75th percentile] range, 5.5 seconds), and mean ± SD recovery time after drug reversal was 147 ± 79 seconds. Heart rate, respiratory rate, and rectal temperature significantly decreased during the anesthetic period. All squirrels became hypothermic by 40 minutes after induction. The righting reflex was absent during the 40-minute anesthetic period in all squirrels, with variable responses for the palpebral reflex, jaw tone, forelimb withdrawal reflex, and hind limb withdrawal reflex. Only 2 of 8 squirrels had loss of the limb withdrawal reflex in both the forelimbs and hind limbs from anesthetic induction to 25 minutes after induction. CONCLUSIONS AND CLINICAL RELEVANCE DKM appeared to provide safe and effective anesthesia in five-striped palm squirrels, but oxygen and thermal support were indicated. At the doses administered, deep surgical anesthesia was not consistently achieved, and anesthetic depth of individual squirrels must be determined before surgical procedures are performed in palm squirrels anesthetized with this drug combination.
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