Water pollution is an urgent problem that needs to be controlled via green transformation and the development of the Yangtze River Economic Belt (YREB). Based on the water pollutant discharge and socio-economic database of prefecture-level cities in the YREB from 2011 to 2015, this study explores the spatiotemporal variations in water pollutant discharge in the YREB via two main indicators: chemical oxygen demand (COD) and ammonia nitrogen (NH₃–N). Further, the spatial effects and determinants of water pollutant discharge are quantitatively estimated. The results show that (1) the water pollutant discharge in the YREB has decreased significantly, with the COD and NH₃–N discharge reduced by 10.46% and 10.79%, respectively, and the discharge reduction in the lower reaches was the most prominent; (2) the spatial pattern of water pollutant discharge in the YREB was generally stable and partially improved, and cities with a high rate of water pollutant reduction in the YREB were distributed in the main stream region of the Yangtze River and the intersection of the main stream and tributaries; (3) spatial effects had a significant impact on water pollutant discharge in the YREB, with regional cooperation and economic radiation through environmental management and control initially showing a combined reduction trend in regional water pollutants; and (4) determinants of population size and agricultural economic share declined to varying degrees at the end of the study period, although the urbanization level continued to increase, indicating that urbanization in the YREB occurred too quickly and that water pollutant discharge reduction was limited. However, economic development leading to the deterioration of the water environment was alleviated. In addition, foreign direct investment (FDI) inflows and rapid industrialization processes must be monitored to increase the reduction in characteristic water pollutants.

High cadmium (Cd) concentration in common wheat (Triticum aestivum L.) grains poses potential health risks. Several management strategies have been used to reduce grain Cd concentration. However, limited information is available on the use of ammonium-nitrogen (NH₄⁺-N) as a strategy to manage Cd concentration in wheat grains. In this study, NH₄⁺-N addition at the seedling stage unchanged the grain Cd concentration in the high-Cd accumulator, Zhoumai 18 (ZM18), but dramatically increased that in the low-Cd accumulator, Yunmai 51 (YM51). Further analysis revealed that the effects of NH₄⁺-N addition on whole-plant Cd absorption, root-to-shoot Cd translocation, and shoot-to-grain Cd remobilization were different between the two wheat cultivars. In ZM18, NH₄⁺-N addition did not change whole-plant Cd absorption, but inhibited root-to-shoot Cd translocation and Cd remobilization from lower internodes, lower leaves, node 1, and internode 1 to grains via the down-regulation of yellow stripe-like transporters (YSL), zinc transporters (ZIP5, ZIP7, and ZIP10), and heavy-metal transporting ATPases (HMA2). This inhibition decreased the grain Cd content by 29.62%, which was consistent with the decrease of the grain dry weight by 23.26%, leading to unchanged grain Cd concentration in ZM18. However, in YM51, NH₄⁺-N addition promoted continuous Cd absorption during grain filling, root-to-shoot Cd translocation and whole-plant Cd absorption. The absorbed Cd was directly transported to internode 1 via the xylem and then re-transported to grains via the phloem by up-regulated YSL, ZIP5, and copper transporters (COPT4). This promotion increased the grain Cd content by 245.35%, which was higher than the increased grain dry weight by 132.89%, leading to increased grain Cd concentration in YM51. Our findings concluded that the addition of NH₄⁺-N fertilizer at the seedling stage is not suitable for reducing grain Cd concentration in common wheat cultivars.

Lake water pollution has caused many serious ecological issues globally. An emerging public concern over water quality deterioration in lakes has heightened the need to evaluate the water quality of lakes at long-term scales, particularly for those with high hydrological alterations. This study combines the Mann–Kendall (M–K) test and self-organising map (SOM) to characterise and evaluate water quality trends in Dongting Lake, China, from 1991 to 2018, before and after the inauguration of the Three Gorges Dam (TGD). Herein, six water quality parameters were selected, namely pH, permanganate index (CODMₙ), ammonia nitrogen (NH₃–N), total nitrogen (TN), total phosphorus (TP), and the five-day biochemical oxygen demand (BOD₅). Our results show that the concentrations of TN and BOD₅ increase significantly throughout the study period (|Z| ≥ 1.96). The number of abrupt change points for the six water quality parameters in the post-TGD period was greater than that in the pre-TGD period, which indicates an increased risk of water deterioration in the post-TGD period. The SOM results show that the pH values ranged from 7.64 to 7.85 among the four clusters; besides, the concentrations of the remaining water quality parameters from 1991 to 1997 and 2000 to 2003 were relatively lower, suggesting that the water quality in the pre-TGD period was better. The classification of TN and TP ranged from Level Ⅳ–Ⅴ among the clusters, which did not satisfy the level Ⅲ standard for potable water, thereby posing a higher ecological risk to the Dongting Lake. These results indicate the deterioration of the water quality in Dongting Lake during the post-TGD period under the influences of pollution load and hydrological regulation. Therefore, strict controls on the external nutrient loading and hydrological regulations should be considered for water quality management.

It is estimated that approximately 0.4% of the total leachate produced in a landfill is destined for treatment plants, while the rest can reach the soil and groundwater. In this context, this study aimed to perform leachate toxicity evaluations through immune system cytotoxic assessments, genotoxic (comet assay) appraisals and antioxidant system (superoxide dismutase - SOD; catalase - CAT, glutathione-S-transferase - GST; reduced glutathione - GSH and metallothionein - MT) evaluations in Eisenia andrei earthworms exposed to a Brazilian leachate for 77 days. The leachate sample contained high organic matter (COD - 10,630 mg L⁻¹) and ammoniacal nitrogen (2398 mg L⁻¹), as well as several metals, including Ca, Cr, Fe, Mg, Ni and Zn. Leachate exposure resulted in SOD activity alterations and increased CAT activity and MT levels. Decreased GST activity and GSH levels were also observed. Antioxidant system alterations due to leachate exposure led to increased malondialdehyde levels as a result of lipid peroxidation after the 77 day-exposure. An inflammatory process was also observed in exposed earthworms, evidenced by increased amoebocyte density, and DNA damage was also noted. This study demonstrates for the first time that sublethal effect assessments in leachate-exposed earthworms comprise an important tool for solid waste management.

Nitrate is the most common contaminant in groundwater in Korea, as well as across the world. Reduction of nitrate to ammonia is one of the options available to remediate groundwater. In this study, nitrate in groundwater was removed using a zero-valent iron (ZVI) containing biochar synthesized by co-pyrolyzing iron oxide and sawdust biomass. Among the various biogases generated during the pyrolysis of biomass, CO and H₂ act as reducing agents to transform iron oxides to ZVI. Approximately 71% of nitrate was reduced to ammonium by ZVI-biochar at initial pH 2.0, and the reduction decreased sharply by the increase in pH. The mass of nitrate-N decreased is exactly same with the mass of ammonia-N formed. However, ammonium remained in the aqueous phase after reduction by ZVI-biochar, and the total nitrogen was not lowered. Acid-washed zeolite adsorbed most ammonium reduced by the ZVI-biochar and maintained the pH to acidic condition to facilitate the reduction of nitrate. The results of this study imply that nitrate-contaminated groundwater can be properly treated within the guidelines of water quality by synthesized ZVI-containing biochar.

Understanding wetland water quality dynamics and associated influencing factors is important to assess the numerous ecosystem services they provide. We present a combined self-organizing map (SOM) and linear mixed-effects model (LMEM) to relate water quality variation of multipond systems (MPSs, a common type of non-floodplain wetlands in agricultural regions of southern China) to their extrinsic and intrinsic influences for the first time. Across the 6 test MPSs with environmental gradients, ammonium nitrogen (NH₄⁺-N), total nitrogen (TN), and total phosphate (TP) almost always exceeded the surface water quality standard (2.0, 2.0, and 0.4 mg/L, respectively) in the up- and midstream ponds, while chlorophyll-a (Chl-a) exhibited hypertrophic state (≥28 μg/L) in the midstream ponds during the wet season. Synergistic influences explained 69±12% and 73±10% of the water quality variations in the wet and dry season, respectively. The adverse, extrinsic influences were generally 1.4, 6.9, 3.2, and 4.3 times of the beneficial, intrinsic influences for NH₄⁺-N, nitrate nitrogen (NO₃⁻-N), TP, and potassium permanganate index (CODMₙ), respectively, although the influencing direction and degree of forest and water area proportion were spatiotemporally unstable. While CODMₙ was primarily linked with rural residential areas in the midstream, higher TN and TP concentrations in the up- and midstream were associated with agricultural land, and NH₄⁺-N reflected a small but non-negligible source of free-range poultry feeding. Pond surface sediments exhibited consistent, adverse effects with amplifications during rainfall, while macrophyte biomass can reflect the biological uptake of CODMₙ and Chl-a, especially in the mid- and downstream during the wet season. Our study advances nonpoint source pollution (NPSP) research for small water bodies, explores nutrient “source-sink” dynamics, and provides a timely guide for rural planning and pond management. The modelling procedures and analytical results can inform refined assessment of similar NFWs elsewhere, where restoration efforts are required.

Enormous wastewater discharges have significantly impeded the sustainable development. As several economic belt has been formed in China, systematic analysis of multi-regional wastewater metabolic system is required for advancing wastewater mitigation effectively and efficiently. In this study, a distributive environmental input-output model (DEIO) is developed for the Yangtze River Economic Belt (YREB) to provide bases for supporting sustainable development from inter-regional and inter-sectoral perspectives. The discharges and flows of wastewater and related pollutants (i.e., chemical oxygen demand (COD) and ammonia nitrogen (AN)) among sectors and regions are analyzed to providing solid bases for wastewater management within the YREB. The results show that the industrial wastewater mitigation in YREB is desired urgently. The industrial wastewater discharges in Jiangsu and Zhejiang provinces are numerous, while Hunan and Yunnan provinces are more inclined to suffer from serious COD and AN pollution. In addition, the manufacture of food, tobacco, chemical materials, and pharmaceutical are the typical sectors with a large amount of direct wastewater discharge, and the tertiary industry is ranked at the first in indirect wastewater discharge. According to the analysis, the implementation of the “Supply-side Structure Reform” and the “Replace Subsidies with Rewards” policy can benefit the wastewater mitigation in the YREB.

Chloropicrin (CP) controls soil-borne plant diseases caused by pathogenic microbes, increases crop yield, but has a long-term inhibitory effect on beneficial soil microorganisms. Therefore, we evaluated the effects of biofumigation material fresh chicken manure (FCM) on soil microorganisms, and the duration of those effects in this experiment. Our results showed that in the laboratory, FCM significantly increased substrate-induced respiration (SIR) of soil microorganisms by 2.2–3.2 times at 80 d compared to the control, however, CP significantly inhibited the SIR of soil microorganisms. FCM and CP increased NH4+-N concentration within 40 days which then returned to the control level. FCM increased NO3--N by 2.82–5.78 times by 80 days, compared with the control, while the concentration of NO3--N in the CP treatment was not significantly different from the control at the 80 day. Although in the laboratory FCM inhibited the relative abundance of 16 S rRNA and the nitrogen cycle functional genes AOA amoA, AOB amoA, nirK and nosZ over a 40-day period, the taxonomic diversity of soil bacteria and fungi in the FCM treatment were restored to unfumigated level within 90 days in the field. However, CP treatment has a strong inhibitory effect on soil microorganisms after 90 days. Importantly, the relative abundance of some beneficial microorganisms that control soil-borne pathogenic microbes or degrade pollutants increased significantly in FCM, including Bacillus, Pseudomonas and Streptomyces bacterial genera and Chaetomium and Mycothermus fungal genera. Noteworthy, like CP, FCM still had a strong inhibitory effect on Fusarium at 90 d. Our results indicated that FCM not only increased the content of inorganic nitrogen and improved the respiration rate of soil microorganisms, but it also shortened the recovery time of beneficial soil microorganisms and increased taxonomic diversity. Our previous reports showed that FCM and CP treatments had the same effect in disease control and crop growth. Combined with the results of this experiment, we believe that FCM has the potential to replace CP, which would eliminate CP's detrimental environmental impact, improve farmer safety and promote sustainable crop production.

In this work, the ammonium-tolerant duckweed Landoltia punctata 0202 was used to study the effect of ammonium stress on carbon and nitrogen metabolism and elucidate the detoxification mechanism. The growth status, protein and starch content, and activity of nitrogen assimilation enzymes were determined, and the transcriptional levels of genes involved in ion transport and carbon and nitrogen metabolism were investigated. Under high ammonium stress, the duckweed growth was inhibited, especially when ammonium was the sole nitrogen source. Ammonium might mainly enter cells via low-affinity transporters. The stimulation of potassium transport genes suggested sufficient potassium acquisition, precluding cation deficiency. In addition, the up-regulation of ammonium assimilation and transamination indicated that excess ammonium could be incorporated into organic nitrogen. Furthermore, the starch content increased from 3.97% to 16.43% and 26.02% in the mixed-nitrogen and ammonium-nitrogen groups, respectively. And the up-regulated starch synthesis, degradation, and glycolysis processes indicated that the accumulated starch could provide sufficient carbon skeletons for excess ammonium assimilation. The findings of this study illustrated that the coordination of carbon and nitrogen metabolism played a vital role in the ammonium detoxification mechanism of duckweeds.

Owing to their high carbon and nitrogen contents, biogas residues may lead to higher carbon dioxide (CO₂) and nitrous oxide (N₂O) emissions from soils. Acidification of biogas slurry and application of nitrification inhibitors (NIs) could mitigate the emission of these gases. An incubation experiment was therefore carried out to investigate the effect of NIs, DMPP (3, 4-dimethylpyrazole phosphate), and PIADIN (active ingredients: 3.00–3.25% 1,2,4-triazole and 1.50–1.65% 3-methylpyrazole), on CO₂ and N₂O emissions from soils fertilized with biogas residues and acidified biogas residues. Biogas residues produced higher ammonium-nitrogen (NH₄⁺-N) and nitrate-nitrogen (NO₃⁻-N) concentrations in soils which resulted in higher emissions of CO₂-C and N₂O-N than that from acidified biogas residues. Both DMPP and PIADIN significantly decreased the emissions of CO₂-C (8.1–55.8%) and N₂O-N (87–98%) and maintained lower NH₄⁺-N and NO₃⁻-N concentrations when compared to control (without nitrification inhibitors). However, the DMPP had a higher reduction capability for CO₂-C emissions than PIADIN in acidified biogas residue applied soil. In conclusion, the acidification of biogas residues and application of NIs are effect in reducing gaseous emission from biogas residue fertilized soils and thus could improve the fertilizer effectiveness of the residues.