Titanium dioxide nanoparticles (TiO₂NPs) application in variety of commercial products would likely release these NPs into the environment. The interaction of TiO₂NPs with terrestrial plants upon uptake can disturb plants functional traits and can also transfer to the food chain members. In this study, we investigated the impact of TiO₂NPs on wheat (Triticum aestivum L.) plants functional traits, primary macronutrients assimilation, and change in the profile of bio-macromolecule. Moreover, the mechanism of biochar-TiO₂NPs interaction, immobilization, and tissue accumulation to cell translocation of NPs in plants was also explored. The results indicated that the contents of Ti in wheat tissues was reduced about 3-fold and the Ti transfer rate (per day) was reduced about 2 fold at the 1000 mg L⁻¹ exposure level of TiO₂NPs in biochar amended exposure medium. Transmission electron microscopy (TEM) with elemental mapping confirmed that Ti concentrated in plant tissues in nano-form. The interactive effect of TiO₂NPs + biochar amendment on photosynthesis related and gas exchange traits was observed at relatively low TiO₂NPs exposure level (200 mg L⁻¹), which induced the positive impact on wheat plants proliferation. TiO₂NPs alone exposure to wheat also modified the plant’s bio-macromolecules profile with the reduction in the assimilation of primary macronutrients, which could affect the food crop nutritional value and quality. X-ray photoelectron spectroscopy (XPS) chemical analysis of biochar + TiO₂NPs showed an additional peak, which indicated the binding interaction of NPs with biochar. Moreover, Fourier-transform infrared (FTIR) spectroscopy confirmed that the biochar carboxyl group is the main functionality involved in the bonding process with TiO₂NPs. These findings will help for a mechanistic understanding of the role of biochar in the reduction of NPs bioavailability to primary producers of the terrestrial environment.

Plastic film mulching is a common practice to increase crop yield in dryland, while the wide use of plastic film has resulted in ubiquitous phthalate esters (PAEs) releasing into the soil. PAEs in soil could be taken up and accumulated by dietary intake of food crops such as wheat, thus imposing health risks to residents. In the present study, samples from a long-term location-fixed field experiment were examined to clarify the accumulation of PAEs in soil and wheat, and to assess the human health risks from PAEs via dietary intake of wheat grain under plastic film mulching cultivation in dryland. Results showed that concentrations of PAEs in grains from mulching plots ranged from 4.1 to 12.6 mg kg−1, which were significantly higher than those in the control group. There was a positive correlation for the PAE concentrations between wheat grains and field soils. Concentrations of PAEs in the soil were in the range of 1.8–3.5 mg kg−1 for the mulching treatment, and 0.9–2.7 mg kg−1 for the control group. Di-n-butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) were detected in all soil and grain samples, and DEHP was found to be the dominant PAE compound in grains. Based on DEHP concentrations in wheat grains, the values of carcinogenic risk for adults were higher than the recommended value 10−4. Results indicated that wheat grains from film mulching plots posed a considerable non-carcinogenic risk to residents, with children being the most sensitive resident group. Findings of this work call the attention to the potential pollution of grain crops growing in the plastic film mulching crop production systems.

Treated wastewater is expected to be increasingly used as an alternative source of irrigation water in areas facing fresh water scarcity. Understanding the behaviors of contaminants from wastewater in soil and plants following irrigation is critical to assess and manage the risks associated with wastewater irrigation. The objective of this study was to evaluate the effects of soil texture and drought stress on the uptake of antibiotics and the internalization of human pathogens into lettuce through root uptake following wastewater irrigation. Lettuce grown in three soils with variability in soil texture (loam, sandy loam, and sand) and under different levels of water stress (no drought control, mild drought, and severe drought) were irrigated with synthetic wastewater containing three antibiotics (sulfamethoxazole, lincomycin and oxytetracycline) and one Salmonella strain a single time prior to harvest. Antibiotic uptake in lettuce was compound-specific and generally low. Only sulfamethoxazole was detected in lettuce with increasing uptake corresponding to increasing sand content in soil. Increased drought stress resulted in increased uptake of lincomycin and decreased uptake of oxytetracycline and sulfamethoxazole. The internalization of Salmonella was highly dependent on the concentration of the pathogen in irrigation water. Irrigation water containing 5 Log CFU/mL Salmonella resulted in limited incidence of internalization. When irrigation water contained 8 Log CFU/mL Salmonella, the internalization frequency was significantly higher in lettuce grown in sand than in loam (p = 0.009), and was significantly higher in lettuce exposed to severe drought than in unstressed lettuce (p = 0.049). This work demonstrated how environmental factors affected the risk of contaminant uptake by food crops following wastewater irrigation.

Many pharmaceutical and personal care products (PPCPs) and endocrine-disrupting chemicals (EDCs) are present in reclaimed water, leading to concerns of human health risks from the consumption of food crops irrigated with reclaimed water. This study evaluated the potential for plant uptake and accumulation of four commonly occurring PPCP/EDCs, i.e., bisphenol A (BPA), diclofenac sodium (DCL), naproxen (NPX), and 4-nonylphenol (NP), by lettuce (Lactuca sativa) and collards (Brassica oleracea) in hydroponic culture, using 14C-labeled compounds. In both plant species, plant accumulation followed the order of BPA > NP > DCL > NPX and accumulation in roots was much greater than in leaves and stems. Concentrations of 14C-PPCP/EDCs in plant tissues ranged from 0.22 ± 0.03 to 927 ± 213 ng/g, but nearly all 14C-residue was non-extractable. PPCP/EDCs, particularly BPA and NP, were also extensively transformed in the nutrient solution. Dietary uptake of these PPCP/EDCs by humans was predicted to be negligible.

Environmental contamination by arsenic constitutes a problem in many countries, and its accumulation in food crops may pose health complications for humans. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are involved at various levels in the mechanism of responding to environmental stress in higher plants. Using Arabidopsis seedlings exposed to different arsenate concentrations, physiological and biochemical parameters were analyzed to determine the status of ROS and RNS metabolisms. Arsenate provoked a significant reduction in growth parameters and an increase in lipid oxidation. These changes were accompanied by an alteration in antioxidative enzymes and the nitric oxide (NO) metabolism, with a significant increase in NO content, S-nitrosoglutathione reductase (GSNOR) activity and protein tyrosine nitration as well as a concomitant reduction in glutathione and S-nitrosoglutathione (GSNO) content. Our results indicate that 500 μM arsenate (AsV) causes nitro-oxidative stress in Arabidopsis, being the glutathione reductase and the GSNOR activities clearly affected.

The co-occurrence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals in agricultural soils has become a worldwide food crop security concern. Pot experiments, rhizosphere microbial metagenomic sequencing, and root metatranscriptomic sequencing were performed to investigate the interactions among pyrene, Cu, and Cd in a soil-maize (Zea mays L.) system. This study provided direct evidence that the co-presence of PAHs and heavy metals changed the root physiological functions and the rhizosphere microbial community, which subsequently influenced the fate of the contaminants. Co-contamination at low levels tended to enhance the uptake potential and biodegradation performance of the plant, whereas increased contaminant concentrations produced opposite effects. The co-presence of 1000 mg/kg Cu decreased the abundance of Mycobacterium in the rhizosphere and reduced pyrene degradation by 12%–16%. The presence of 400–750 mg/kg pyrene altered the metabolic processes, molecular binding functions, and catalytic activity of enzymes in the maize roots, thus impeding the phytoextraction of Cu and Cd. Competitive absorption between Cu and Cd was observed for the 800–1000 mg/kg Cu and 50–100 mg/kg Cd co-treatment, in which Cu showed a competitive advantage, enhancing its root-to-shoot translocation. These findings provide important information for the production of safe crops and for the development of phytoremediation technologies.

Due to the potential toxicity of polycyclic aromatic hydrocarbons (PAHs) to humans, the uptake and translocation of PAHs in food crops have gained much attention. However, it is still unclear whether phloem participates in the acropetal translocation of PAHs in plants. Herein, the evidence for acropetal translocation of phenanthrene (a model PAH) via phloem is firstly tested. Wheat (Triticum aestivum L.) new leaves contain significantly higher phenanthrene concentration than old leaves (P < 0.05), and the inhibitory effect on phenanthrene translocation is stronger in old leaves after abscisic acid and polyvinyl alcohol (two common transpiration inhibitors) application. Phenanthrene concentration in xylem sap is slightly higher than in phloem sap. Ring-girdling treatment can significantly reduce phenanthrene concentration in castor bean (Ricinus communis L.) leaves. Two-photon fluorescence microscope images indicate a xylem-to-phloem and acropetal phloem translocation of phenanthrene in castor bean stem. Therefore, phloem is involved in the acropetal translocation of phenanthrene in wheat seedlings, especially when the xylem is not mature enough in scattered vascular bundle plants. Our results provide a deeper understanding of PAH translocation in plants, which have significant implications for food safety and phytoremediation enhancement of PAH-contaminated soil and water.

Wastewater reuse in food crop irrigation has led to agroecosystem pollution concerns and human health risks. However, there is limited attention on the relationship of sub-lethal antibiotic levels in vegetables and resistance selection. Most risk assessment studies show non-significant toxicity, but overlook the link between antibiotics in crops and propagation of gut microbiome resistance selection. The review highlights the risk of antibiotics in treated water used for irrigation, uptake, and accumulation in edible vegetable parts. Moreover, it elucidates the risks to the adaptive resistance selection of the gut microbiome from sub-lethal antibiotic levels, as a result of dietary contaminated vegetables. Experiments have reported that bacterial resistance selection is possible at concentrations that are several hundred-folds lower than lethal effect levels on susceptible cells. Consequently, mutants selected at low antibiotic levels, such as those from vegetables, are fitter and more resistant compared to those selected at high concentrations. Necessary standardization, such as the development of minimum acceptable antibiotic limits allowable in food crop irrigation water, with a focus on minimum selection concentration, and not only toxicity, has been proposed. Wastewater irrigation offers environmental benefits and can contribute to food security, but it has non-addressed risks. Research gaps, future perspectives, and frameworks of mitigating the potential risks are discussed.

Cadmium (Cd) is a toxic metal that contributes to human diseases such as pediatric cancer and cardiovascular dysfunction. Epigenetic modification caused by Cd exposure is the major factor in etiology of environmentally-relevant diseases. However, the underlying epigenetic mechanism for Cd uptake and accumulation in food crops, particularly those growing in Cd-contaminated environments, is largely unknown. This study investigated uncharacterized regulatory mechanisms and biological functions of global DNA hypomethylation at CG sites that are associated with gene expression for Cd detoxification and accumulation in the food crop rice. Mutation of the CG maintenance enzyme OsMET1 confers rice tolerance to Cd exposure. Genome-wide analysis of OsMET1 loss of function mutant Osmet1 and its wild type shows numerous loci differentially methylated and upregulated genes for Cd detoxification, transport and accumulation. We functionally identified a new locus for a putative cadmium tolerance factor (here termed as OsCTF) and demonstrated that Cd-induced DNA demethylation is the drive of OsCTF expression. The 3′-UTR of OsCTF is the primary site of DNA and histone (H3K9me2) demethylation, which is associated with higher levels of OsCTF transcripts detected in the Osmet1 and Ossdg714 mutant lines. Mutation of OsCTF in rice led to hypersensitivity to Cd and the Osctf line accumulated more Cd, whereas transfer of OsCTF back to the Osctf mutant completely restored the normal phenotype. Our work unveiled an important epigenetic mechanism and will help develop breeding crops that contribute to food security and better human health.

In order to tackle China’s severe air pollution issue, the government has released the “Air Pollution Prevention and Control Action Plan” (known simply as the “Action Plan”) since 2013. A recent study reported a decreased trend in PM₂.₅ concentrations over 2013–2017, but O₃ pollution has become more serious. However, the effects of surface O₃ on crops are unclear after the implementation of the “Action Plan”. Here, we evaluated the potential negative effects of surface O₃ on three main food crops (winter wheat, maize and rice) across China during 2015–2018 using nationwide O₃ monitoring data and AOT40-yield response functions. Results suggested that mean O₃ concentration, AOT40 and relative yield loss in China showed an overall upward trend from 2015 to 2018. During winter wheat, maize, single rice, double-early rice, and double-late rice growing seasons, mean O₃ concentration in recent years ranged from 38.6 to 46.9 ppb, 40.2–43.9 ppb, 39.3–42.2 ppb, 33.8–40.0 ppb, and 35.9–39.1 ppb, respectively, and AOT40 mean values ranged from 8.5 to 14.3 ppm h, 10.5–13.4 ppm h, 9.8–11.9 ppm h, 5.2–9.2 ppm h, and 8.0–9.5 ppm h, respectively. O₃-induced yield reductions were estimated to range from 20.1 to 33.3% for winter wheat, 5.0–6.3% for maize, 7.3–8.8% for single rice, 3.9–6.8% for double-early rice and 5.9–7.1% for double-late rice. O₃-induced production losses for winter wheat, maize, single rice, double-early rice, and double-late rice totaled 39.5–88.2 million metric tons, 12.6–21.0 million metric tons, 9.5–11.3 million metric tons, 1.2–1.8 million metric tons, and 2.2–2.7 million metric tons, respectively, and the corresponding economic losses totaled 14.3–32.0 billion US$, 3.9–6.5 billion US$, 3.9–4.6 billion US$, 0.5–0.7 billion US$, and 0.9–1.1 billion US$, respectively. Our results suggested that the government should take effective measures to reduce O₃ pollution and its effects on agricultural production.