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Reactivity of adenosine triphosphatase adsorbed on nitrocellulose
2001
Momic, T. | Banjac, A. | Horvat, A. (Institut za nuklearna istrazivanja Vinca, Beograd (Yugoslavia). Laboratorija za molekularnu biologiju)
Possibilities of application of transmembrane enzymes as a biological component of a biosensor for water quality control and detection of toxical substances were performed. Synaptic plasma membranes (SPMs) were adsorbed on nitrocellulose filters. The adsorption of SPMs was followed by determination of the transmembrane enzyme Na,K-ATPase. The optimal conditions for SPM adsorption on nitrocellulose filters were determined: 25 microgram per nitrocellulose filter disc during 1 hour of incubation, on - 20 deg C. The ATPase activity of adsorbed SPM showed, that almost 30% of enzymic activity was detected on nitrocellulose filters in mentioned conditions. This results showed that adsorption of SPM on solid support enhancing enzymatic stability and enable its industrial and analytical application.
Show more [+] Less [-]Application of membrane processes in potable water treatment
2003
Cakic, B. | Popovic, L. | Ljuboja, M. | Svrkota, D. (Institut za vodoprivredu "Jaroslav Cerni", Beograd (Serbia and Montenegro))
Membrane processes is gaining broad application in potable water treatment. Experience in application of membrane system for treatment of potable water so far showed that these systems have wide range of advantages versus conventional treatment: lower capital costs, lower operation costs, use of chemicals is minimal; space for installation is much smaller, usage of chlorine for disinfection is minimal due to significant reduction of microorganisms; sludge production during the process is smaller due to smaller usage of chemicals, construction period is shorter due to smaller scope of works and relatively simply installation of membrane batteries.
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