Low flows are a good measure of the waste water recipient self-purification capability. The regional statistical analysis is an objective way of assessment of the required low flows. This paper presents the principles and key phases of the regional statistical analysis. The advantages of this method are a reduction of the outliers influence and the assessment of low flows on ungauged streams and stream profiles. The method is illustrated with the results obtained by the required low flows regional statistical analysis in Serbia (Yugoslavia) with 59 hydrological gauge stations in the scope, which data has the same length of 39 years, from the year 1956 to 1994.

As a result of this study the overload by pollutants and low quality of canal water in the zone downstream of Vrbas and Srbobran towns (Serbia, Yugoslavia) has been recorded. Regarding to started work on demudification of canal bottom in the industrial zone of Vrbas town, in the course of 2000 the additional investigations of mud profile downstrean of wastewater discharge into the canal have been done. In the investigated mud samples the extremely high number of organotrophic bacteria was found (up to 1.7 x 10**10 cfu/ml), but also high number of conditionally pathogenic bacteria (opportunistic bacteria) was recorded. Also, the high number of fecal (thermo-tolerant) coliform bacteria (Escherichia coli and Klebsiella - Enterobacter - Citrobacter group of bacteria), as well as the presence of colifags was found. Such findings indicate possible bad consequences to the health of humans contacting this mud, as well as to the ground water quality, indirectly, via drainage water.

Drainage of industrial and communal effluents, directly into the water flow, as many hydrotechnical activities, significantly influence the schedule and balance of the processes that take place into water. In this paper, there are represented the results of systematic control of the water quality of the Danube river and DTD (Danube-Tisza-Danube) channel on the parts Vrbas-Bezdan and Becej-Bogojevo, at the control station network of the Republic Weather Bureau (the Republic of Serbia), for the period of 1991-1996. The results show that the most adverse influence of anthropogenic factor is on the part of the flow of the DTD (Danube-Tisza-Danube) channel from Vrbas to the flow into Tisza river.

Spectrophotometric method for determination of inorganic phosphate liberated in hydrolysis of ATP catalyzed by ATPase was modified in order to obtain faster procedure, which could also be used in none laboratory conditions. The modification has some advantages compared to the most used Pennial method: a) the reagents are stable for several months; b) the 45 min procedure of phosphomolibdate extraction by isobutanole-benzene is ommited, and the method is not dangerous for the analyst; c) color develops after 20 min. The method was tested on the determination of inorganic phosphate in the presence of cadmium nitrate as inhibitor of ATPase activity. The results were compared to the results obtained by Pennial method. The results obtained have shown some good agreements.

In our earlier work, we have shown that enzymes from rat brain synaptosomal membranes, Na(+)/K(+)-ATPase and Mg(2+)-ATPase, are promising biological components of a biosensor for lead detection. In this work, we represent our results of investigation with the same enzymes as biological components for the biosensor in presence of Hg(2+) ions in water. It was established that IC50 for Na(+)/K(+)-ATPase and Mg(2+)-ATPase is 6.9 and 5.5 x 10E-6 M, and the percentages of inhibition are 96% and 77% respectively. We concluded that these enzymes could be the base for developing biosensors for the presence of Hg(2+) ion in water. Since these enzymes maintain a stable activity for a longer period of time, they could be appropriate as components of biosensors for monitoring water quality.

MPI - Metal Pollution Index is being proposed with an aim improving the freshwater pollution control, monitoring and classification based on fish metal body burden. This is a simple mathematical model which enables presentation of Cd, Pb, Cu, Zn and Al fish liver concentration as a single value. After thorough studies of bioaccumulation, biomagnification and tissue distribution patterns, liver of Carassius auratus gibelio has been chosen as sentinel organ. Normal distribution and biological variation assessment is being achieved by using normalizers - reference values (liver concentrations of selected elements in fish from reference sites Vruje and Moharac micro water reservoirs, Serbia (Yugoslavia)). Selected representative sites have been evaluated according to new classification of freshwater ecosystem based on MPI values.

Oxygen aspect of water pollution of Skadar lake, Montenegro (Yugoslavia) is considered in this paper. Water analysis data in the period of 1991-1998 were used to establish relevant parameters, oxygen saturation (%O2), and five day biochemical demind (BPK5). Those data were supplied by Hydrometeorological Institute in Podgorica, Montenegro (Yugoslavia). Measurements were taken from three places in northern part of Skadar lake, where the increased water pollution is expected, and from southeastern part of the lake, out of source of pollution directly influence. According to results from this research, there is close relation between water pollution and oxygen water balance. Parts of the Skadar lake with low water circulation are especialy susceptible (place Kamenik). In the 1991-1995 period, this phenomena is manifested with variation depending of the chosen area and year. After some improvement of water quality in the 1996-1997 period, there is noticeable increase of water pollution in 1998 at all places. Supersaturation was identified by the percentage of O2 in Skadar lake area at whole.

The experiment was performed on marine Teleost Serranus scriba with lower concentrations of lindane then MPC (due to our valid law). The aim of this study was to find the effects of lindane to fish activities connected with nutrition. This motive is among the strongest. It includes parameters as: number of fish movements in the presence of prey or without it and the number of onslaughts to the prey during one hour. The results have shown that the increasing of lindane concentration caused considerable decreasing and even complete cessation of activity. Interest for prey and decreasing activity of predators in the presence of prey (typical for Serranus - as a fish of prey and the hunter) endures till the moment of convulsion.

In this paper we have pointed out the hydrogeological condition in the catchment area of the Jablanica river in the south Serbia (Yugoslavia). The two units are separately presented here. The first one is mountainous part of the catchment area upstream of the gaging station of Lebane and the second one is the part of the terrain in the plain down stream to the river mounth near Pecenjevac. The hydrogeological characteristics of the two mentioned regions are given to explain the reasons of existing the constant flow of the Jablanica to Lebane and its disappearing course in the period of recession in the plain downstream. The quality of water in the Jablanica river at Lebane is good. Down stream of this gaging station the waste water collector flows into the river. In the part of recession period the ratio between them is unfavourable. We have also discussed the quality of water at the gaging station of Pecenjevac and pointed out the function of sandy-gravel deposits in the process of natural purification of the Jablanica flow.