In this work, ZnO–CdWO₄ nanoparticles have been synthesized by the ecofriendly green method with lemon leaf extract to favorably anchor functional groups on their surface. The prepared ZnO–CdWO₄ nanoparticles are used as adsorbent to treat Congo red (CR) dye after characterization through FT-IR, UV–Vis, TEM, SEM-EDX, and HRTEM techniques. The equilibrium partition coefficient and adsorption capacity values for CR by ZnO–CdWO₄ are estimated as 21.4 mg g⁻¹ μM⁻¹ and 5 mg g⁻¹, respectively (at an initial dye concentration of 10 mg L⁻¹). The adsorption process is found as exothermic and spontaneous, as determined by the ΔG°, ΔS°, and ΔH° values. The Boyd plot has been used as a confirmatory tool to fit the adsorption kinetics data along with intraparticle diffusion and pseudo-second-order models. Based on this research, ZnO–CdWO₄ nanoparticles are validated as an effective adsorbent for CR dye in aqueous solutions.

Mercury (Hg) is a neurotoxic element with severe effects on humans and wildlife. Widely distributed by atmospheric deposition, it can also be localized near point sources such as mines. Mercury has been shown to reduce the reproduction of bird populations in field observations in North America and Europe, but studies are needed in Asia, where the majority of emissions now occur. We investigated the reproduction of two passerines, Japanese Tit (Parus minor) and Russet Sparrow (Passer rutilans), in a large-scale Hg mining district, and a non-mining district, both in Guizhou, southwest China. Concentrations of Hg were elevated in the mining district (blood levels of 2.54 ± 2.21 [SD] and 0.71 ± 0.40 μg/g, in adult tits and sparrows, respectively). However, we saw no evidence of decreased breeding there: metrics such as egg volume, nestling weight, hatching and fledgling success, were all similar between the different districts across two breeding seasons. Nor were there correlations at the mining district between Hg levels of adults or juveniles, and hatching or fledgling success, or nestling weight. Nest success was high even in the mining district (tit, 64.0%; sparrow: 83.1%). This lack of reproductive decline may be related to lower blood levels in nestlings (means < 0.15 μg/g for both species). Concentrations of selenium (Se), and Se-to-Hg molar ratio, were also not correlated to breeding success. Although blood levels of 3.0 μg/g have been considered as a threshold of adverse effects in birds, even leading to severe effects, we detected no population-level reproductive effects, despite ~25% of the adult tits being above this level. Future work should investigate different locations in the mining district, different life-stages of the birds, and a wider variety of species. The hypothesis that bird populations can evolve resistance to Hg in contaminated areas should also be examined further.

Hooghly River (HR), the other name used for the lower stretch of River Ganga, is a prime freshwater source in the eastern part of India. However HR has been evidenced with a variety of emerging organic pollutants (EOPs) in the recent past. Given the extensive use of plasticizers and additive in plastic products, we have investigated seven plasticizers and bisphenol A (BPA) in the surface and storm-water of HR up to the tip of the Bay of Bengal. Further using a previously published sediment data we have estimated the fluxes for the aforementioned EOPs. Surface water and storm-water concentrations of seven plasticizers varied between 92.62 and 770 ng/L (176.1 ± 104.8; Avg ± SD) and 120.9–781.5 ng/L (355.2 ± 232.5), respectively. BPA varied between 43 and 8800 ng/L (658.3 ng/L ± 1760) and 117.9–2147 ng/L (459.3 ± 620.2) in surface and storm-water, respectively. With the increase in salinity, a decreasing trend for bis-(2-ethylhexyl) phthalate (DEHP) was evidenced. However, concentration of BPA increased with the increase in salinity. Significant and strong correlation between DEHP and BPA (R² = 0.6; p < 0.01) in the suburban corridor might have resulted from sludge disposal of the scrap recycling activities. Using site-specific principal component analysis, unregulated disposal of plastic waste, particularly from such industrial belts and tourist spots were identified as the possible point sources for plasticizers and BPA in this region. Net diffusive flux based on fugacity fraction showed a trend depending on the pollutant’s aqueous solubility and partition coefficient. However, transfer tendency from water to sediment was noticed in the sites having point source. Estimated ecotoxicological risk posed by BPA was higher for edible fishes and for lower order organisms, PAEs was the major contributor.

Polychlorinated biphenyls (PCBs) may transfer into the neonates through the placental transfer and via breastfeeding after the delivery, thus might be harmful to the infant. Sixty colostrum samples in the Yangtze River Region were collected to investigate the concentration, distribution pattern, and enantiomer characteristic of the PCB exposure. Among all samples, over 90% of pollutants were tetra-to hepta-chlorinated PCBs. The sum concentration of the PCB was 512 (IQR: 322–856) ng g⁻¹ lipid weight. Enantiomer fraction (EF) of PCB 95 and PCB 149 was found lower than the racemic value, while EFs of PCB 45 and PCB 136 were found higher and near-racemic state, respectively. The concentration pattern and enantiomeric properties of the PCBs indicated that the mothers from Mianyang had a recent exposure to PCBs. Among all samples, similar exposure and metabolic pathways of the PCB congeners were observed. PCB exposure showed no significant correlation with the birth outcome of the infants, but 43.3% of the infants have potential health risks via breastfeeding.

To date, micropollutants from anthropogenic sources cannot be completely removed from effluents of wastewater treatment plants and therefore enter freshwater systems, where they may impose adverse effects on aquatic organisms, for example, on fish. Advanced treatment such as ozonation aims to reduce micropollutants in wastewater effluents and, thus, to mitigate adverse effects on the environment. To investigate the impact and efficiency of ozonation, four different water types were tested: ozonated wastewater (before and after biological treatment), conventionally-treated wastewater, and water from a river (River Ruhr, Germany) upstream of the wastewater treatment plant effluent. Zebrafish (Danio rerio) embryos were used to study lethal and sublethal effects in a modified fish early life-stage test. Mortality occurred during exposure in the water samples from the wastewater treatment plant and the river in the first 24 h post-fertilization, ranging from 12% (conventional wastewater) to 40% (river water). Regarding sublethal endpoints, effects compared to the negative control resulted in significantly higher heart rates (ozonated wastewater), and significantly reduced swimming activity (highly significant in ozonated wastewater and ozone reactor water, significant in only the last time interval in river water). Moreover, the respiration rates were highly increased in both ozonated wastewater samples in comparison to the negative control. Significant differences between the ozonated wastewater samples occurred in the embryonic behavior and heart rates, emphasizing the importance of subsequent biological treatment of the ozonated wastewater. Only the conventionally-treated wastewater sample did not elicit negative responses in zebrafish, indicating that the discharge of conventional wastewater poses no greater risk to embryonic and larval zebrafish than water from the river Ruhr itself. The sublethal endpoints embryonic- and larval behavior, heart rates, and respiration were found to be the most sensitive endpoints in this fish early life-stage test and can add valuable information on the toxicity of environmental samples.

Lake water pollution has caused many serious ecological issues globally. An emerging public concern over water quality deterioration in lakes has heightened the need to evaluate the water quality of lakes at long-term scales, particularly for those with high hydrological alterations. This study combines the Mann–Kendall (M–K) test and self-organising map (SOM) to characterise and evaluate water quality trends in Dongting Lake, China, from 1991 to 2018, before and after the inauguration of the Three Gorges Dam (TGD). Herein, six water quality parameters were selected, namely pH, permanganate index (CODMₙ), ammonia nitrogen (NH₃–N), total nitrogen (TN), total phosphorus (TP), and the five-day biochemical oxygen demand (BOD₅). Our results show that the concentrations of TN and BOD₅ increase significantly throughout the study period (|Z| ≥ 1.96). The number of abrupt change points for the six water quality parameters in the post-TGD period was greater than that in the pre-TGD period, which indicates an increased risk of water deterioration in the post-TGD period. The SOM results show that the pH values ranged from 7.64 to 7.85 among the four clusters; besides, the concentrations of the remaining water quality parameters from 1991 to 1997 and 2000 to 2003 were relatively lower, suggesting that the water quality in the pre-TGD period was better. The classification of TN and TP ranged from Level Ⅳ–Ⅴ among the clusters, which did not satisfy the level Ⅲ standard for potable water, thereby posing a higher ecological risk to the Dongting Lake. These results indicate the deterioration of the water quality in Dongting Lake during the post-TGD period under the influences of pollution load and hydrological regulation. Therefore, strict controls on the external nutrient loading and hydrological regulations should be considered for water quality management.

One of the causes of the global decline of amphibians is agricultural activity, responsible for causing habitat fragmentation and bringing a range of agrochemicals and fertilizers in the environment, compounds with a potential disrupting effect on non-target organisms, such as frogs. Exposure to these compounds has numerous harmful effects on the testes of these animals, which can compromise reproduction and, consequently, the maintenance of their communities. In this context, we compared the morphology and morphometry of the testes of three species of neotropical anurans (Physalaemus cuvieri, Dendropsophus minutus, and Boana albopunctata) from an agricultural area and a conservation unit. Histologically, the testicular morphology of the species was similar for both environments; however, morphometrically, there was a difference in the measured testicular parameters (locular area and area of spermatogenic cysts). Physalaemus cuvieri presented higher averages of locular and spermatogonial area in the agricultural environment, whereas the area occupied by the spermatozoa was smaller. Additionally, the testicular pigmentation, which is only present in this species, was greater in animal from the agricultural area. In D. minutus, the locular, spermatogonial, and sperm areas showed lower values in the agricultural area, whereas in B. albopunctata, the opposite pattern was found, with the area of the locule, spermatocytes, and spermatozoids being higher. Agricultural activities influence the testicular metric parameters in different species, and our results suggest that D. minutus is most sensitive to anthropic pressures. The least sensitive species is B. albopunctata. We highlight the importance of evaluating different species, since each species responds differently to agricultural activities.

Aging is a leading cause of mortality for the elderly and DNA methylation age is reported to be predictive of biological aging. However, few studies have investigated the associations between multiple metals exposure and accelerated aging in the elderly. We performed a pilot study of 288 elderly participants aged 50–115 years and measured genome-wide DNA methylation and 22 blood metals concentrations. Measures of DNA methylation age were estimated using CpGs from Illumina HumanMethylation EPIC BeadChip. Linear mixed regression and Bayesian kernel machine regression (BKMR) models were used to estimate the individual and overall associations between multiple metals and accelerated methylation aging. Single metal models revealed that each 1-standard deviance (SD) increase in log-transformed vanadium, cobalt, nickel, zinc, arsenic, and barium was associated with a −2.256, −1.318, 1.004, −1.926, 1.910 and −1.356 changes in ΔAge, respectively; meanwhile, for aging rate, the change was −0.019, −0.013, 0.010, −0.018, 0.023, and −0.012, respectively (all P < 0.05). The BKMR models showed reverse U-shaped associations of the overall metals mixture with ΔAge and aging rate. Downward trends of ΔAge and aging rate were observed for increasing quantiles of essential metals mixture, but upward trends were observed for non-essential metals mixture. Further individual analysis of the BKMR revealed that the 95% confidence interval of ΔAge and aging rate associated with vanadium, zinc, and arsenic did not cross 0, when other metals concentrations set at 25th, 50th, and 75th percentile. Our findings suggest reverse U-shaped associations of the overall metals mixture with accelerated methylation aging for the first time, and vanadium, zinc, and arsenic may be major contributors driving the associations.

Bats are poorly understood as a reservoir of multidrug-resistant strains; therefore, the aim of this study was to determine molecular characterization of multidrug-resistant Enterococcus strains isolated from bat species from Poland. A multi-stage analysis based on targeted isolation of drug-resistant strains (selective media with tetracycline, chloramphenicol, gentamicin, streptomycin, and vancomycin), determination of the phenotypic profile of drug-susceptibility using the disc diffusion method, and amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) was used for the isolation and differentiation of strains. The applied strategy finally allowed identification of E. faecalis resistant to at least one antimicrobial in 47.2% of the single-animal group and in 46.9% of the pooled samples of bat’s guano. Out of the 36 distinct isolates, 69% met the criteria of multi-drug resistance, with a dominant combination of resistance to tetracycline, erythromycin, and rifampicin. Simultaneously, 41.6% of the strains were high-level aminoglycoside resistant (HLAR). In most strains, phenotypic resistance was reflected in the presence of at least one gene encoding resistance to a given drug. Moreover, our research results show that some genes were detected simultaneously in the same strain statistically significantly more frequently. This may confirm that the spread of some genes (tetM and ermB or aph (3′)-IIIa as well as gelE and aac (6′)-Ie-aph (2″)-Ia or ant (6)-Ia) is associated with their common occurrence on the same mobile genetic element.To our knowledge, this is the first analysis of multidrug-resistance among E. faecalis isolated from bats. Our research demonstrates that the One Health concept is not associated exclusively with food-producing animals and humans, but other species of wildlife animals should be covered by monitoring programs as well.We confirmed for the first time that bats are an important reservoir of multi-resistant E. faecalis strains and could have a great impact on environmental resistance.

We examined polycyclic aromatic compounds (PACs) and petroleum biomarkers (steranes, hopanes, and terpanes) in radiometrically-dated lake sediment cores from the Athabasca oil sands region (AOSR) and the Peace-Athabasca Delta (PAD) region in Alberta (Canada) to determine whether contributions from petroleum hydrocarbons have changed over time. Two floodplain lakes in the PAD (PAD 30, PAD 31) recorded increased flux of alkylated PACs and increased petrogenic (petroleum-derived) hydrocarbons after ∼1980, coincident with a decline of sediment organic carbon content and a rise of bulk sedimentation rate, likely due to increased Athabasca River flow. A large expansion of upstream oilsands mining, upgrading, and refining may also have contributed to the observed shift to more petrogenic hydrocarbons to sediments since the 1980s. Alkylated PAC flux increased in the floodplain lake analyzed within the AOSR (Saline Lake) since the 1970s–1980s, coincident with a sharp rise in sediment organic carbon content and increased contributions of petrogenic hydrocarbons. These changes identify increased supply of petrogenic PACs occurred as Athabasca River floodwaters waned, and may implicate aerial contributions of petrogenic hydrocarbons from oilsands activity. PACs and petroleum biomarkers (steranes, hopanes, and terpanes) in sediment cores from Saline Lake, PAD 30 and PAD 31 revealed a predominance of petrogenic hydrocarbons in these lakes. In contrast, we recorded minimal petrogenic hydrocarbons in the reference lakes outside the surface minable area of the AOSR and PAD (Mariana Lake and BM11), though we noted slight increases in petrogenic contributions to modern (2010–2016) sediments. We show how a combined analysis of PACs and petroleum biomarkers in sediments is useful to quantify petrogenic contributions to lakes with added confidence and highlight the potential for petroleum biomarkers in lake sediment cores as a novel and effective method to track petroleum hydrocarbons in lake sediment.